1WSF

Co-crystal structure of E.coli RNase HI active site mutant (D134A*) with Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Detector technology	IMAGE PLATE
Collection date	2003-11-28
Detector	MACSCIENCE
Wavelength(s)	0.9
Spacegroup name	P 21 21 2
Unit cell lengths	120.990, 80.089, 63.951
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
Rwork	0.216
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300	2.300
Number of reflections	25555
Completeness [%]	90.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1