1V5I
Crystal structure of serine protease inhibitor POIA1 in complex with subtilisin BPN'
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Subtilisin BPN' | polymer | 275 | 27600.6 | 1 | UniProt (P00782) Pfam (PF00082) | Bacillus amyloliquefaciens | |
| 2 | B (B) | IA-1=serine proteinase inhibitor | polymer | 76 | 8355.3 | 1 | UniProt (Q7M4T6) | Pleurotus ostreatus (oyster mushroom) | POIA1 |
| 3 | C, R (A, B) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 4 | D (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 5 | E, F, G, H, I... (A, B) | GLYCEROL | non-polymer | 92.1 | 16 | Chemie (GOL) | |||
| 6 | V, W (A, B) | water | water | 18.0 | 405 | Chemie (HOH) |
Sequence modifications
A: 1 - 275 (UniProt: P00782)
B: 1 - 76 (UniProt: Q7M4T6)
| PDB | External Database | Details |
|---|---|---|
| Csd 221 | Ser 328 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| His 71 | Gly 71 | engineered mutation |
| Val 72 | Ile 72 | engineered mutation |
| Ala 73 | Val 73 | engineered mutation |
| His 74 | Thr 74 | engineered mutation |
| Ala 75 | Thr 75 | engineered mutation |
| Tyr 76 | Gln 76 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 35955.9 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 1705.7 | |
| All* | Total formula weight | 37661.6 |
