1V5I
Crystal structure of serine protease inhibitor POIA1 in complex with subtilisin BPN'
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Wavelength(s) | 0.8 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.700, 71.800, 94.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.900 - 1.500 |
R-factor | 0.1799 |
Rwork | 0.180 |
R-free | 0.19200 |
Structure solution method | MOLECULAR REPLACEMENT |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HEPES, lithium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |