1V5I
Crystal structure of serine protease inhibitor POIA1 in complex with subtilisin BPN'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.700, 71.800, 94.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.900 - 1.500 |
| R-factor | 0.1799 |
| Rwork | 0.180 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HEPES, lithium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
