1TL7

Complex Of Gs- With The Catalytic Domains Of Mammalian Adenylyl Cyclase: Complex With 2'(3')-O-(N-methylanthraniloyl)-guanosine 5'-triphosphate and Mn

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Adenylate cyclase, type V	polymer	217	24495.4	1	UniProt (P30803) Pfam (PF00211)	Canis lupus familiaris (dog)	ATP pyrophosphate-lyase, Ca2+, -inhibitable adenylyl cyclase
2	B (B)	Adenylate cyclase, type II	polymer	212	23717.0	1	UniProt (P26769) Pfam (PF00211)	Rattus norvegicus (Norway rat)	ATP pyrophosphate-lyase, Adenylyl cyclase
3	C (C)	Guanine nucleotide-binding protein G(s), alpha subunit	polymer	402	46656.4	1	UniProt (P04896) Pfam (PF00503)	Bos taurus (cattle)	Adenylate cyclase-stimulating G alpha protein
4	D, E (A)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
5	F (A)	FORSKOLIN	non-polymer		410.5	1	Chemie (FOK)
6	G (A)	3'-O-(N-METHYLANTHRANILOYL)-GUANOSINE-5'-TRIPHOSPHATE	non-polymer		656.3	1	Chemie (ONM)
7	H (C)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
8	I (C)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
9	J (C)	5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE	non-polymer		539.2	1	Chemie (GSP)
10	K, L, M (A, B, C)	water	water		18.0	18	Chemie (HOH)

Sequence modifications

A: 364 - 580 (UniProt: P30803)

PDB	External Database	Details
Met 476	Val 557	conflict

C: 1 - 394 (UniProt: P04896)

PDB	External Database	Details
Gly 71	71	conflict
-	Gln 79	deletion
Asn 79	Ala 80	conflict
Lys 81	Arg 82	conflict
Gly 395	-	expression tag
Gly 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	94868.8
Non-Polymers*	Number of molecules	7
	Total formula weight	1775.7
All*	Total formula weight	96644.5

*Water molecules are not included.