1TL7
Complex Of Gs- With The Catalytic Domains Of Mammalian Adenylyl Cyclase: Complex With 2'(3')-O-(N-methylanthraniloyl)-guanosine 5'-triphosphate and Mn
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-23 |
Detector | SBC-3 |
Wavelength(s) | 1.0393 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 118.230, 133.600, 70.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.990 - 2.800 |
R-factor | 0.254 |
Rwork | 0.254 |
R-free | 0.29800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PBD entry 1AZS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.910 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.379 | |
Number of reflections | 22694 | |
<I/σ(I)> | 7.34 | 1.44 |
Completeness [%] | 66.2 | |
Redundancy | 3.4 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | 7.5-7.8% PEG 8000, 0.5M NACL, 0.1M PHOSPHATE BUFFER, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |