1T05

HIV-1 reverse transcriptase crosslinked to template-primer with tenofovir-diphosphate bound as the incoming nucleotide substrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	T	oligonucleotide template	polymer	27	8383.4	1
2	P	oligonucleotide primer	polymer	21	6474.3	1
3	A	POL polyprotein	polymer	558	64249.7	1	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) In PDB	Human immunodeficiency virus 1
4	B	POL polyprotein	polymer	437	51123.6	1	UniProt (P04585) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) In PDB	Human immunodeficiency virus 1
5	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	A	[2-(6-AMINO-9H-PURIN-9-YL)-1-METHYLETHOXY]METHYL-TRIPHOSPHATE	non-polymer		447.2	1	Chemie (TNV)
7	A, B	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
8		water	water		18.0	21	Chemie (HOH)

Sequence modifications

A: 1 - 558 (UniProt: P03366)

PDB	External Database	Details
Cys 258	Gln 425	engineered mutation
Ser 280	Cys 447	engineered mutation

B: 1 - 429 (UniProt: P04585)

PDB	External Database	Details
Ser 280	Cys 435	engineered mutation
Gly 430	-	cloning artifact
Gly 431	-	cloning artifact
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	130230.9
Non-Polymers*	Number of molecules	8
	Total formula weight	956.3
All*	Total formula weight	131187.2

*Water molecules are not included.