1SXJ

Crystal Structure of the Eukaryotic Clamp Loader (Replication Factor C, RFC) Bound to the DNA Sliding Clamp (Proliferating Cell Nuclear Antigen, PCNA)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Activator 1 95 kDa subunit	polymer	516	56378.0	1	UniProt (P38630) Pfam (PF00004) Pfam (PF08519) In PDB	Saccharomyces cerevisiae (baker's yeast)	Replication factor C subunit 1, Replication factor C1, Cell division control protein 44
2	B	Activator 1 37 kDa subunit	polymer	323	36172.0	1	UniProt (P40339) Pfam (PF00004) Pfam (PF08542) In PDB	Saccharomyces cerevisiae (baker's yeast)	Replication factor C subunit 4, Replication factor C4
3	C	Activator 1 40 kDa subunit	polymer	340	38225.5	1	UniProt (P38629) Pfam (PF00004) Pfam (PF08542) In PDB	Saccharomyces cerevisiae (baker's yeast)	Replication factor C subunit 3, Replication factor C3
4	D	Activator 1 41 kDa subunit	polymer	353	39765.4	1	UniProt (P40348) Pfam (PF00004) Pfam (PF08542) In PDB	Saccharomyces cerevisiae (baker's yeast)	Replication factor C subunit 2, Replication factor C2
5	E	Activator 1 40 kDa subunit	polymer	354	39964.5	1	UniProt (P38251) Pfam (PF13177) In PDB	Saccharomyces cerevisiae (baker's yeast)	Replication factor C subunit 5, Replication factor C5
6	F, G, H	Proliferating cell nuclear antigen	polymer	283	32053.2	3	UniProt (P15873) Pfam (PF00705) Pfam (PF02747) In PDB	Saccharomyces cerevisiae (baker's yeast)	PCNA
7	A, B, C, D	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
8	A, B, C, D	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	4	Chemie (AGS)
9	E	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)

Sequence modifications

A: 295 - 785 (UniProt: P38630)

PDB	External Database	Details
Met 270	-	expression tag
Gly 271	-	expression tag
Ser 272	-	expression tag
Ser 273	-	expression tag
His 274	-	expression tag
His 275	-	expression tag
His 276	-	expression tag
His 277	-	expression tag
His 278	-	expression tag
His 279	-	expression tag
Ser 280	-	expression tag
Ser 281	-	expression tag
Gly 282	-	expression tag
Leu 283	-	expression tag
Glu 284	-	expression tag
Val 285	-	expression tag
Leu 286	-	expression tag
Phe 287	-	expression tag
Gln 288	-	expression tag
Gly 289	-	expression tag
Pro 290	-	expression tag
His 291	-	expression tag
Met 292	-	expression tag
Ala 293	-	expression tag
Ser 294	-	expression tag

B: 1 - 323 (UniProt: P40339)

PDB	External Database	Details
Gln 157	Arg 157	engineered mutation

C: 1 - 340 (UniProt: P38629)

PDB	External Database	Details
Gln 160	Arg 160	engineered mutation

D: 1 - 353 (UniProt: P40348)

PDB	External Database	Details
Gln 183	Arg 183	engineered mutation

E: 1 - 354 (UniProt: P38251)

PDB	External Database	Details
Gln 184	Arg 184	engineered mutation

F, G, H: 1 - 258 (UniProt: P15873)

PDB	External Database	Details
Mse -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
Ser -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Leu -11	-	expression tag
Glu -10	-	expression tag
Val -9	-	expression tag
Leu -8	-	expression tag
Phe -7	-	expression tag
Gln -6	-	expression tag
Gly -5	-	expression tag
Pro -4	-	expression tag
His -3	-	expression tag
Mse -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Mse 1	Met 1	modified residue
Mse 70	Met 70	modified residue
Mse 119	Met 119	modified residue
Mse 161	Met 161	modified residue
Mse 188	Met 188	modified residue
Mse 199	Met 199	modified residue

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	306665.0
Non-Polymers*	Number of molecules	9
	Total formula weight	2617.4
All*	Total formula weight	309282.4

*Water molecules are not included.