Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1SXJ

Crystal Structure of the Eukaryotic Clamp Loader (Replication Factor C, RFC) Bound to the DNA Sliding Clamp (Proliferating Cell Nuclear Antigen, PCNA)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003689	molecular_function	DNA clamp loader activity
A	0005524	molecular_function	ATP binding
A	0005663	cellular_component	DNA replication factor C complex
A	0006260	biological_process	DNA replication
A	0006281	biological_process	DNA repair
A	0016887	molecular_function	ATP hydrolysis activity
B	0000077	biological_process	DNA damage checkpoint signaling
B	0003677	molecular_function	DNA binding
B	0003689	molecular_function	DNA clamp loader activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005663	cellular_component	DNA replication factor C complex
B	0005829	cellular_component	cytosol
B	0006260	biological_process	DNA replication
B	0006261	biological_process	DNA-templated DNA replication
B	0006271	biological_process	DNA strand elongation involved in DNA replication
B	0006272	biological_process	leading strand elongation
B	0006281	biological_process	DNA repair
B	0007062	biological_process	sister chromatid cohesion
B	0007064	biological_process	mitotic sister chromatid cohesion
B	0016887	molecular_function	ATP hydrolysis activity
B	0031389	cellular_component	Rad17 RFC-like complex
B	0031390	cellular_component	Ctf18 RFC-like complex
B	0031391	cellular_component	Elg1 RFC-like complex
B	0090618	biological_process	DNA clamp unloading
C	0000077	biological_process	DNA damage checkpoint signaling
C	0003677	molecular_function	DNA binding
C	0003689	molecular_function	DNA clamp loader activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005663	cellular_component	DNA replication factor C complex
C	0005829	cellular_component	cytosol
C	0006260	biological_process	DNA replication
C	0006261	biological_process	DNA-templated DNA replication
C	0006271	biological_process	DNA strand elongation involved in DNA replication
C	0006272	biological_process	leading strand elongation
C	0006281	biological_process	DNA repair
C	0006298	biological_process	mismatch repair
C	0007064	biological_process	mitotic sister chromatid cohesion
C	0016887	molecular_function	ATP hydrolysis activity
C	0031389	cellular_component	Rad17 RFC-like complex
C	0031390	cellular_component	Ctf18 RFC-like complex
C	0031391	cellular_component	Elg1 RFC-like complex
C	0090618	biological_process	DNA clamp unloading
D	0000076	biological_process	DNA replication checkpoint signaling
D	0000077	biological_process	DNA damage checkpoint signaling
D	0003677	molecular_function	DNA binding
D	0003689	molecular_function	DNA clamp loader activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005663	cellular_component	DNA replication factor C complex
D	0006260	biological_process	DNA replication
D	0006261	biological_process	DNA-templated DNA replication
D	0006271	biological_process	DNA strand elongation involved in DNA replication
D	0006272	biological_process	leading strand elongation
D	0006281	biological_process	DNA repair
D	0006298	biological_process	mismatch repair
D	0007062	biological_process	sister chromatid cohesion
D	0007064	biological_process	mitotic sister chromatid cohesion
D	0016887	molecular_function	ATP hydrolysis activity
D	0031389	cellular_component	Rad17 RFC-like complex
D	0031390	cellular_component	Ctf18 RFC-like complex
D	0031391	cellular_component	Elg1 RFC-like complex
D	0090618	biological_process	DNA clamp unloading
E	0000077	biological_process	DNA damage checkpoint signaling
E	0003677	molecular_function	DNA binding
E	0003689	molecular_function	DNA clamp loader activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005663	cellular_component	DNA replication factor C complex
E	0006260	biological_process	DNA replication
E	0006261	biological_process	DNA-templated DNA replication
E	0006271	biological_process	DNA strand elongation involved in DNA replication
E	0006272	biological_process	leading strand elongation
E	0006281	biological_process	DNA repair
E	0007062	biological_process	sister chromatid cohesion
E	0007064	biological_process	mitotic sister chromatid cohesion
E	0016887	molecular_function	ATP hydrolysis activity
E	0031389	cellular_component	Rad17 RFC-like complex
E	0031390	cellular_component	Ctf18 RFC-like complex
E	0031391	cellular_component	Elg1 RFC-like complex
E	0090618	biological_process	DNA clamp unloading
F	0000278	biological_process	mitotic cell cycle
F	0000710	biological_process	meiotic mismatch repair
F	0000781	cellular_component	chromosome, telomeric region
F	0003677	molecular_function	DNA binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005657	cellular_component	replication fork
F	0006260	biological_process	DNA replication
F	0006272	biological_process	leading strand elongation
F	0006273	biological_process	lagging strand elongation
F	0006275	biological_process	regulation of DNA replication
F	0006281	biological_process	DNA repair
F	0006289	biological_process	nucleotide-excision repair
F	0006298	biological_process	mismatch repair
F	0006301	biological_process	postreplication repair
F	0007064	biological_process	mitotic sister chromatid cohesion
F	0019985	biological_process	translesion synthesis
F	0030337	molecular_function	DNA polymerase processivity factor activity
F	0030466	biological_process	silent mating-type cassette heterochromatin formation
F	0031509	biological_process	subtelomeric heterochromatin formation
F	0034087	biological_process	establishment of mitotic sister chromatid cohesion
F	0035753	biological_process	maintenance of DNA trinucleotide repeats
F	0042802	molecular_function	identical protein binding
F	0043626	cellular_component	PCNA complex
F	0045739	biological_process	positive regulation of DNA repair
F	0045740	biological_process	positive regulation of DNA replication
F	0051054	biological_process	positive regulation of DNA metabolic process
F	0070987	biological_process	error-free translesion synthesis
G	0000278	biological_process	mitotic cell cycle
G	0000710	biological_process	meiotic mismatch repair
G	0000781	cellular_component	chromosome, telomeric region
G	0003677	molecular_function	DNA binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005657	cellular_component	replication fork
G	0006260	biological_process	DNA replication
G	0006272	biological_process	leading strand elongation
G	0006273	biological_process	lagging strand elongation
G	0006275	biological_process	regulation of DNA replication
G	0006281	biological_process	DNA repair
G	0006289	biological_process	nucleotide-excision repair
G	0006298	biological_process	mismatch repair
G	0006301	biological_process	postreplication repair
G	0007064	biological_process	mitotic sister chromatid cohesion
G	0019985	biological_process	translesion synthesis
G	0030337	molecular_function	DNA polymerase processivity factor activity
G	0030466	biological_process	silent mating-type cassette heterochromatin formation
G	0031509	biological_process	subtelomeric heterochromatin formation
G	0034087	biological_process	establishment of mitotic sister chromatid cohesion
G	0035753	biological_process	maintenance of DNA trinucleotide repeats
G	0042802	molecular_function	identical protein binding
G	0043626	cellular_component	PCNA complex
G	0045739	biological_process	positive regulation of DNA repair
G	0045740	biological_process	positive regulation of DNA replication
G	0051054	biological_process	positive regulation of DNA metabolic process
G	0070987	biological_process	error-free translesion synthesis
H	0000278	biological_process	mitotic cell cycle
H	0000710	biological_process	meiotic mismatch repair
H	0000781	cellular_component	chromosome, telomeric region
H	0003677	molecular_function	DNA binding
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005657	cellular_component	replication fork
H	0006260	biological_process	DNA replication
H	0006272	biological_process	leading strand elongation
H	0006273	biological_process	lagging strand elongation
H	0006275	biological_process	regulation of DNA replication
H	0006281	biological_process	DNA repair
H	0006289	biological_process	nucleotide-excision repair
H	0006298	biological_process	mismatch repair
H	0006301	biological_process	postreplication repair
H	0007064	biological_process	mitotic sister chromatid cohesion
H	0019985	biological_process	translesion synthesis
H	0030337	molecular_function	DNA polymerase processivity factor activity
H	0030466	biological_process	silent mating-type cassette heterochromatin formation
H	0031509	biological_process	subtelomeric heterochromatin formation
H	0034087	biological_process	establishment of mitotic sister chromatid cohesion
H	0035753	biological_process	maintenance of DNA trinucleotide repeats
H	0042802	molecular_function	identical protein binding
H	0043626	cellular_component	PCNA complex
H	0045739	biological_process	positive regulation of DNA repair
H	0045740	biological_process	positive regulation of DNA replication
H	0051054	biological_process	positive regulation of DNA metabolic process
H	0070987	biological_process	error-free translesion synthesis

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG A 811

Chain	Residue
A	THR360
A	AGS801

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG B 812

Chain	Residue
B	THR56
B	ASP114
B	AGS802
C	ARG131

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG C 813

Chain	Residue
C	THR60
C	ASP117
C	AGS803

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG D 814

Chain	Residue
D	THR72
D	ASP140
D	GLU141
D	AGS804
E	ARG155

site_id	AC5
Number of Residues	19
Details	BINDING SITE FOR RESIDUE AGS A 801

Chain	Residue
A	THR299
A	TYR302
A	ALA303
A	PRO304
A	GLN309
A	VAL310
A	CYS311
A	GLY356
A	ILE357
A	GLY358
A	LYS359
A	THR360
A	THR361
A	ASN456
A	ILE514
A	ARG515
A	MG811
B	ARG128
B	GLU132

site_id	AC6
Number of Residues	20
Details	BINDING SITE FOR RESIDUE AGS B 802

Chain	Residue
B	VAL12
B	TYR15
B	ARG16
B	PRO17
B	ILE23
B	VAL24
B	GLY25
B	GLY52
B	ILE53
B	GLY54
B	LYS55
B	THR56
B	THR57
B	ASN145
B	ARG174
B	MET202
B	ARG203
B	MG812
C	ARG131
C	ALA156

site_id	AC7
Number of Residues	20
Details	BINDING SITE FOR RESIDUE AGS C 803

Chain	Residue
C	VAL16
C	TYR19
C	ARG20
C	PRO21
C	VAL27
C	TYR28
C	PRO55
C	GLY56
C	THR57
C	GLY58
C	LYS59
C	THR60
C	SER61
C	ASN148
C	LEU169
C	MET205
C	ARG206
C	LEU209
C	MG813
D	ARG154

site_id	AC8
Number of Residues	21
Details	BINDING SITE FOR RESIDUE AGS D 804

Chain	Residue
D	VAL28
D	GLU29
D	TYR31
D	ARG32
D	PRO33
D	VAL39
D	THR40
D	ALA41
D	PRO67
D	GLY68
D	THR69
D	GLY70
D	LYS71
D	THR72
D	SER73
D	ASN171
D	LEU228
D	ARG229
D	MG814
E	ARG155
E	PRO180

site_id	AC9
Number of Residues	17
Details	BINDING SITE FOR RESIDUE ADP E 805

Chain	Residue
E	PRO44
E	ASN45
E	GLY46
E	THR47
E	GLY48
E	LYS49
E	LYS50
E	THR51
E	ILE201
E	LEU230
E	ARG231
E	LEU234
E	VAL5
E	ARG9
E	PRO10
E	SER17
E	HIS18

Functional Information from PROSITE/UniProt

site_id	PS00293
Number of Residues	19
Details	PCNA_2 Proliferating cell nuclear antigen signature 2. RCDHpvtLgmdLtSLsKIL

Chain	Residue	Details
F	ARG61-LEU79

site_id	PS01251
Number of Residues	24
Details	PCNA_1 Proliferating cell nuclear antigen signature 1. GIiAqAVDdSRVlLVsLeIgveaF

Chain	Residue	Details
F	GLY34-PHE57

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	57
Details	DNA_BIND: DNA_BIND => ECO:0000255

Chain	Residue	Details
F	ARG61-ARG80
G	ARG61-ARG80
H	ARG61-ARG80
E	ARG231
D	ARG229
C	ARG206

site_id	SWS_FT_FI2
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) => ECO:0000269\|PubMed:15166219

Chain	Residue	Details
F	LYS127
G	LYS127
H	LYS127

site_id	SWS_FT_FI3
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000269\|PubMed:12226657

Chain	Residue	Details
F	LYS164
G	LYS164
H	LYS164

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1nsf

Chain	Residue	Details
C	ARG206
C	LYS59
C	ARG132

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1nsf

Chain	Residue	Details
A	ASN456
A	ARG515

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1nsf

Chain	Residue	Details
A	ARG515
A	SER448

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1nsf

Chain	Residue	Details
E	ASP172
E	ARG231

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)