1R8S
ARF1[DELTA1-17]-GDP IN COMPLEX WITH A SEC7 DOMAIN CARRYING THE MUTATION OF THE CATALYTIC GLUTAMATE TO LYSINE
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | ADP-ribosylation factor 1 | polymer | 164 | 18822.5 | 1 | UniProt (P84080) Pfam (PF00025) | Bos taurus (cattle) | |
| 2 | B (E) | Arno | polymer | 203 | 23438.7 | 1 | UniProt (Q99418) Pfam (PF01369) | Homo sapiens (human) | ARF nucleotide-binding site opener, cytohesin 2, ARF exchange factor |
| 3 | C (A) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 4 | D (A) | BETA-MERCAPTOETHANOL | non-polymer | 78.1 | 1 | Chemie (BME) | |||
| 5 | E, F, I (A, E) | FORMIC ACID | non-polymer | 46.0 | 3 | Chemie (FMT) | |||
| 6 | G (E) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 7 | H (E) | SULFITE ION | non-polymer | 80.1 | 1 | Chemie (SO3) | |||
| 8 | J, K (A, E) | water | water | 18.0 | 350 | Chemie (HOH) |
Sequence modifications
E: 50 - 252 (UniProt: Q99418)
| PDB | External Database | Details |
|---|---|---|
| Lys 156 | Glu 156 | engineered mutation |
| Tyr 190 | Phe 190 | engineered mutation |
| Ser 191 | Ala 191 | engineered mutation |
| Asp 198 | Ser 198 | engineered mutation |
| Met 208 | Pro 208 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 42261.2 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 835.5 | |
| All* | Total formula weight | 43096.7 |
