1R8S
ARF1[DELTA1-17]-GDP IN COMPLEX WITH A SEC7 DOMAIN CARRYING THE MUTATION OF THE CATALYTIC GLUTAMATE TO LYSINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 104.018, 104.018, 69.277 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 * - 1.460 |
| R-factor | 0.15941 |
| Rwork | 0.159 |
| R-free | 0.17300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1R8R and crystal structure of the Arf1[D1-17]/Gea2-Sec7 complex (Goldberg J. (1998) Cell 95(2):237-48) |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.421 |
| Data scaling software | XDS |
| Phasing software | CCP4 ((MOLREP)) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.470 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Number of reflections | 75051 | |
| <I/σ(I)> | 26.6 | 5.9 |
| Completeness [%] | 97 | |
| Redundancy | 16 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 16 * | 1.88M Ammonium Sulfate, 100mM MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.88 (M) | |
| 2 | 1 | reservoir | MES | 100 (%) | pH6. |
