1MW8
Crystal Structure of a Complex between H365R mutant of 67 kDA N-terminal fragment of E. coli DNA Topoisomerase I and 5'-ACTTCGGGATG-3'
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (Y) | 5'-D(*AP*CP*TP*TP*CP*GP*GP*GP*AP*TP*G)-3' | polymer | 11 | 3389.2 | 1 | |||
| 2 | B (X) | DNA Topoisomerase I | polymer | 592 | 66930.8 | 1 | UniProt (P06612) Pfam (PF01751) Pfam (PF01131) | Escherichia coli | Omega-protein, Relaxing enzyme, Untwisting enzyme, Swivelase |
| 3 | C, D, E, F, G (X) | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
| 4 | H (X) | THYMIDINE-5'-PHOSPHATE | non-polymer | 322.2 | 1 | Chemie (TMP) PubChem (1139) PubChem (444919) PubChem (5748250) PubChem (10147784) PubChem (18531120) PubChem (20711568) PubChem (23657880) PubChem (24802545) PubChem (44361457) PubChem (25113134) PubChem (9700) PubChem (51397487) PubChem (5274091) PubChem (129069890) PubChem (133126478) PubChem (164603144) | |||
| 5 | I, J (Y, X) | water | water | 18.0 | 553 | Chemie (HOH) |
Sequence modifications
X: 1 - 592 (UniProt: P06612)
| PDB | External Database | Details |
|---|---|---|
| Arg 365 | His 365 | engineered mutation |
| Asn 549 | Ser 549 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 70320.0 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 802.5 | |
| All* | Total formula weight | 71122.6 |
