1MW8
Crystal Structure of a Complex between H365R mutant of 67 kDA N-terminal fragment of E. coli DNA Topoisomerase I and 5'-ACTTCGGGATG-3'
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (Y) | 5'-D(*AP*CP*TP*TP*CP*GP*GP*GP*AP*TP*G)-3' | polymer | 11 | 3389.2 | 1 | |||
| 2 | B (X) | DNA Topoisomerase I | polymer | 592 | 66930.8 | 1 | UniProt (P06612) Pfam (PF01751) Pfam (PF01131) | Escherichia coli | Omega-protein, Relaxing enzyme, Untwisting enzyme, Swivelase |
| 3 | C, D, E, F, G (X) | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
| 4 | H (X) | THYMIDINE-5'-PHOSPHATE | non-polymer | 322.2 | 1 | Chemie (TMP) | |||
| 5 | I, J (Y, X) | water | water | 18.0 | 553 | Chemie (HOH) |
Sequence modifications
X: 1 - 592 (UniProt: P06612)
| PDB | External Database | Details |
|---|---|---|
| Arg 365 | His 365 | engineered mutation |
| Asn 549 | Ser 549 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 70320.0 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 802.5 | |
| All* | Total formula weight | 71122.6 |
