1MW8
Crystal Structure of a Complex between H365R mutant of 67 kDA N-terminal fragment of E. coli DNA Topoisomerase I and 5'-ACTTCGGGATG-3'
Functional Information from GO Data
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 X 2002 |
| Chain | Residue |
| X | LYS28 |
| X | SER29 |
| X | LYS251 |
| X | HOH1338 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 X 2004 |
| Chain | Residue |
| X | TRP560 |
| X | LYS561 |
| X | HOH1041 |
| X | HOH1138 |
| X | HOH1231 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 X 2005 |
| Chain | Residue |
| X | ARG515 |
| X | ARG516 |
| X | HOH1257 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 X 2007 |
| Chain | Residue |
| X | ARG202 |
| X | ALA458 |
| X | ARG535 |
| X | ASN539 |
| X | HOH1043 |
| X | HOH1252 |
| X | HOH1429 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 X 2008 |
| Chain | Residue |
| X | HIS469 |
| X | LYS472 |
| X | HOH1512 |
| site_id | AC6 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE TMP X 600 |
| Chain | Residue |
| X | ASP113 |
| X | ARG114 |
| X | GLU115 |
| X | ARG161 |
| X | GLN165 |
| X | ARG168 |
| X | ARG365 |
| X | SER495 |
| X | ALA554 |
| X | HOH1092 |
| X | HOH1139 |
| X | HOH1271 |
| X | HOH1345 |
| X | HOH1373 |
| X | HOH1405 |
| X | HOH1433 |
| X | HOH1477 |
Functional Information from PROSITE/UniProt
| site_id | PS00396 |
| Number of Residues | 15 |
| Details | TOPO_IA_1 Topoisomerase (Topo) IA-type active site signature. QrLYEagy.........ITYmRTD |
| Chain | Residue | Details |
| X | GLN309-ASP323 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 5 |
| Details | Region: {"description":"Interaction with DNA"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | Active site: {"description":"O-(5'-phospho-DNA)-tyrosine intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU01383","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"21482796","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8114910","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9497321","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00952","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 8 |
| Details | Site: {"description":"Interaction with DNA"} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1ecl |
| Chain | Residue | Details |
| X | ASP111 | |
| X | TYR319 | |
| X | GLU9 | |
| X | ARG365 |
| site_id | MCSA1 |
| Number of Residues | 7 |
| Details | M-CSA 366 |
| Chain | Residue | Details |
