1M6V

Crystal Structure of the G359F (small subunit) Point Mutant of Carbamoyl Phosphate Synthetase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, E, G)	carbamoyl phosphate synthetase large chain	polymer	1073	117982.7	4	UniProt (P00968) Pfam (PF02786) Pfam (PF02787) Pfam (PF02142)	Escherichia coli	Carbamoyl-phosphate synthetase ammonia chain
2	B, D, F, H (B, D, F, H)	carbamoyl-phosphate synthetase small chain	polymer	382	41571.0	4	UniProt (P00907) Pfam (PF00988) Pfam (PF00117) Pfam (PF07722) UniProt (by SIFTS) (P0A6F1)	Escherichia coli	Carbamoyl-phosphate synthetase glutamine chain
3	AB, CA, DA, DB, GA... (E, C, A, G)	MANGANESE (II) ION	non-polymer		54.9	12	Chemie (MN)
4	AA, AC, BB, BC, CB... (B, G, E, C, A...)	POTASSIUM ION	non-polymer		39.1	26	Chemie (K)
5	BA, EC, FC, GC, HC... (B, G, E, C, H...)	CHLORIDE ION	non-polymer		35.5	28	Chemie (CL)
6	KC, OB, SA, V (G, E, C, A)	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4)
7	LC, MC, PB, QB, TA... (G, E, C, A)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	8	Chemie (ADP)
8	NC, RB, VA, Y (G, E, C, A)	L-ornithine	non-polymer		132.2	4	Chemie (ORN)
9	OC, SB, WA, Z (G, E, C, A)	TETRAETHYLAMMONIUM ION	non-polymer		130.3	4	Chemie (NET)
10	QC, RC, SC, TC, UC... (A, B, C, D, E...)	water	water		18.0	3561	Chemie (HOH)

Sequence modifications

A, C, E, G: 1 - 1073 (UniProt: P00968)

PDB	External Database	Details
Asn 46	Leu 45	SEE REMARK 999

B, D, F, H: 1 - 382 (UniProt: P00907)

PDB	External Database	Details
Gln 183	Glu 183	SEE REMARK 999
Phe 359	Gly 359	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	638214.8
Non-Polymers*	Number of molecules	86
	Total formula weight	7515.6
All*	Total formula weight	645730.4

*Water molecules are not included.