1M5K

Crystal structure of a hairpin ribozyme in the catalytically-active conformation

Replaces:  1HP6

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, D)	RNA INHIBITOR SUBSTRATE	polymer	21	6739.9	2
2	B, D (B, E)	RNA HAIRPIN RIBOZYME	polymer	92	29969.8	2
3	E, F (C, F)	PROTEIN (U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A)	polymer	100	11498.5	2	UniProt (P09012) Pfam (PF00076)	Homo sapiens (human)	U1 snRNP A protein; U1 snRNP-specific protein A
4	AA, BA, CA, DA, EA... (E, A, B, C, F...)	CALCIUM ION	non-polymer		40.1	33	Chemie (CA)
5	NA, OA (F)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6	PA, QA, RA, SA, TA... (A, B, D, E, C...)	water	water		18.0	103	Chemie (HOH)

Sequence modifications

C, F: 1 - 100 (UniProt: P09012)

PDB	External Database	Details
His 31	Tyr 31	engineered mutation
Arg 36	Gln 36	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	96416.2
Non-Polymers*	Number of molecules	35
	Total formula weight	1393.5
All*	Total formula weight	97809.7

*Water molecules are not included.