Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2000-10-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 256.300, 44.100, 102.400 |
| Unit cell angles | 90.00, 109.00, 90.00 |
Refinement procedure
| Resolution | 61.070 - 2.400 |
| R-factor | 0.23 |
| Rwork | 0.229 |
| R-free | 0.28500 * |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 * | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.067 * | 0.261 |
| Number of reflections | 41290 * | |
| <I/σ(I)> | 22.8 | 2.1 |
| Completeness [%] | 95.6 * | 73.9 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5 | 22 * | Rupert, P.B., (2001) Nature, 410, 780. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | CaCl2 | ||
| 2 | 1 | 1 | NH4Cl | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | CaCl2 | ||
| 5 | 1 | 2 | NH4Cl | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 2.5 (mM) | ||
| 2 | 1 | reservoir | 20 (mM) | ||
| 3 | 1 | reservoir | 2 (mM) | ||
| 4 | 1 | reservoir | 150-190 (mM) | ||
| 5 | 1 | reservoir | MPD | 21 (%) |
