1LWG

Multiple Methionine Substitutions are Tolerated in T4 Lysozyme and have Coupled Effects on Folding and Stability

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Lysozyme	polymer	164	18800.7	1	UniProt (P00720) Pfam (PF00959)	Enterobacteria phage T4	Lysis protein, Muramidase, Endolysin
2	B (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
3	C, D, F (A)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
4	E (A)	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
5	G, H, I (A)	2-HYDROXYETHYL DISULFIDE	non-polymer		154.3	3	Chemie (HED)
6	J (A)	water	water		18.0	144	Chemie (HOH)

Sequence modifications

A: 1 - 164 (UniProt: P00720)

PDB	External Database	Details
Thr 54	Cys 54	engineered mutation
Met 84	Leu 84	engineered mutation
Met 87	Val 87	engineered mutation
Met 91	Leu 91	engineered mutation
Ala 97	Cys 97	engineered mutation
Met 99	Leu 99	engineered mutation
Met 111	Val 111	engineered mutation
Met 118	Leu 118	engineered mutation
Met 121	Leu 121	engineered mutation
Met 133	Leu 133	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	18800.7
Non-Polymers*	Number of molecules	8
	Total formula weight	703.2
All*	Total formula weight	19503.9

*Water molecules are not included.