1LWG

Multiple Methionine Substitutions are Tolerated in T4 Lysozyme and have Coupled Effects on Folding and Stability

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003796	molecular_function	lysozyme activity
A	0003824	molecular_function	catalytic activity
A	0009253	biological_process	peptidoglycan catabolic process
A	0016787	molecular_function	hydrolase activity
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0016998	biological_process	cell wall macromolecule catabolic process
A	0030430	cellular_component	host cell cytoplasm
A	0031640	biological_process	killing of cells of another organism
A	0042742	biological_process	defense response to bacterium
A	0044659	biological_process	viral release from host cell by cytolysis

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 A 170

Chain	Residue
A	SER90
A	THR142
A	PRO143
A	ASN144
A	ARG145

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CL A 227

Chain	Residue
A	HIS31
A	LYS135
A	HOH171
A	HOH190

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CL A 228

Chain	Residue
A	LYS65
A	ASN68
A	VAL94
A	TRP158

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE K A 325

Chain	Residue
A	GLU11
A	GLY12
A	TYR18
A	GLY28
A	ILE29
A	GLY30

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CL A 330

Chain	Residue
A	LYS65
A	GLN69
A	ASP92
A	ALA93
A	GLN141

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE HED A 901

Chain	Residue
A	PHE4
A	ASP72
A	VAL75
A	TYR88
A	ALA93
A	HOH174

---

site_id	AC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE HED A 902

Chain	Residue
A	LYS83
A	MET111
A	THR115
A	MET118
A	ARG119
A	GLN122
A	HOH513

---

site_id	AC8
Number of Residues	8
Details	BINDING SITE FOR RESIDUE HED A 903

Chain	Residue
A	ILE9
A	ASP10
A	GLU11
A	TYR18
A	GLN123
A	LYS124
A	HOH281
A	HOH302

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_04110","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"3382407","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7831309","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8266098","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_04110","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"1892846","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"3382407","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7831309","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8266098","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"8266098","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"7831309","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 206l

Chain	Residue	Details
A	GLU11
A	ASP20

---

site_id	MCSA1
Number of Residues	2
Details	M-CSA 921

Chain	Residue	Details
A	GLU11	proton shuttle (general acid/base)
A	ASP20	covalent catalysis

---