1L0O
Crystal Structure of the Bacillus stearothermophilus Anti-Sigma Factor SpoIIAB with the Sporulation Sigma Factor SigmaF
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Anti-sigma F factor | polymer | 150 | 16765.9 | 2 | UniProt (O32727) Pfam (PF02518) In PDB | Geobacillus stearothermophilus | SpoIIAB, Stage II sporulation protein AB |
2 | C | sigma factor | polymer | 243 | 27939.8 | 1 | UniProt (O32728) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB | Geobacillus stearothermophilus | SpoIIAC, sigma F |
3 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
4 | A, B | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 2 | Chemie (ADP) | |||
5 | water | water | 18.0 | 83 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 142 (UniProt: O32727)
C: 7 - 245 (UniProt: O32728)
PDB | External Database | Details |
---|---|---|
Met 34 | Thr 34 | SEE REMARK 999 |
His 137 | Ala 137 | SEE REMARK 999 |
Ile 138 | Tyr 138 | SEE REMARK 999 |
Val 139 | Cys 139 | SEE REMARK 999 |
Lys 140 | Glu 140 | SEE REMARK 999 |
Ser 141 | Lys 141 | SEE REMARK 999 |
Lys 142 | Gln 142 | SEE REMARK 999 |
Arg 143 | - | cloning artifact |
Tyr 144 | - | cloning artifact |
Leu 145 | - | cloning artifact |
Glu 146 | - | cloning artifact |
Gly 147 | - | cloning artifact |
Ser 148 | - | cloning artifact |
Ser 149 | - | cloning artifact |
Phe 150 | - | cloning artifact |
PDB | External Database | Details |
---|---|---|
Gly 3 | - | cloning artifact |
Ser 4 | - | cloning artifact |
His 5 | - | cloning artifact |
Met 6 | - | cloning artifact |
Ala 132 | Ser 132 | SEE REMARK 999 |
Val 135 | Ile 135 | SEE REMARK 999 |
Lys 215 | Arg 215 | SEE REMARK 999 |
Gln 217 | Arg 217 | SEE REMARK 999 |
Met 233 | Val 233 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 61471.7 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 903.0 | |
All* | Total formula weight | 62374.7 |