1L0O

Crystal Structure of the Bacillus stearothermophilus Anti-Sigma Factor SpoIIAB with the Sporulation Sigma Factor SigmaF

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Anti-sigma F factor	polymer	150	16765.9	2	UniProt (O32727) Pfam (PF02518)	Geobacillus stearothermophilus	SpoIIAB, Stage II sporulation protein AB
2	C (C)	sigma factor	polymer	243	27939.8	1	UniProt (O32728) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545)	Geobacillus stearothermophilus	SpoIIAC, sigma F
3	D, F (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	E, G (A, B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
5	H, I, J (A, B, C)	water	water		18.0	83	Chemie (HOH)

Sequence modifications

A, B: 1 - 142 (UniProt: O32727)

PDB	External Database	Details
Met 34	Thr 34	SEE REMARK 999
His 137	Ala 137	SEE REMARK 999
Ile 138	Tyr 138	SEE REMARK 999
Val 139	Cys 139	SEE REMARK 999
Lys 140	Glu 140	SEE REMARK 999
Ser 141	Lys 141	SEE REMARK 999
Lys 142	Gln 142	SEE REMARK 999
Arg 143	-	cloning artifact
Tyr 144	-	cloning artifact
Leu 145	-	cloning artifact
Glu 146	-	cloning artifact
Gly 147	-	cloning artifact
Ser 148	-	cloning artifact
Ser 149	-	cloning artifact
Phe 150	-	cloning artifact

C: 7 - 245 (UniProt: O32728)

PDB	External Database	Details
Gly 3	-	cloning artifact
Ser 4	-	cloning artifact
His 5	-	cloning artifact
Met 6	-	cloning artifact
Ala 132	Ser 132	SEE REMARK 999
Val 135	Ile 135	SEE REMARK 999
Lys 215	Arg 215	SEE REMARK 999
Gln 217	Arg 217	SEE REMARK 999
Met 233	Val 233	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	61471.7
Non-Polymers*	Number of molecules	4
	Total formula weight	903.0
All*	Total formula weight	62374.7

*Water molecules are not included.