1L0O
Crystal Structure of the Bacillus stearothermophilus Anti-Sigma Factor SpoIIAB with the Sporulation Sigma Factor SigmaF
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004672 | molecular_function | protein kinase activity |
| A | 0004674 | molecular_function | protein serine/threonine kinase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0006468 | biological_process | protein phosphorylation |
| A | 0010468 | biological_process | regulation of gene expression |
| A | 0016989 | molecular_function | sigma factor antagonist activity |
| A | 0030436 | biological_process | asexual sporulation |
| A | 0042174 | biological_process | negative regulation of sporulation resulting in formation of a cellular spore |
| A | 0045892 | biological_process | negative regulation of DNA-templated transcription |
| A | 0106310 | molecular_function | protein serine kinase activity |
| B | 0004672 | molecular_function | protein kinase activity |
| B | 0004674 | molecular_function | protein serine/threonine kinase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0006468 | biological_process | protein phosphorylation |
| B | 0010468 | biological_process | regulation of gene expression |
| B | 0016989 | molecular_function | sigma factor antagonist activity |
| B | 0030436 | biological_process | asexual sporulation |
| B | 0042174 | biological_process | negative regulation of sporulation resulting in formation of a cellular spore |
| B | 0045892 | biological_process | negative regulation of DNA-templated transcription |
| B | 0106310 | molecular_function | protein serine kinase activity |
| C | 0003677 | molecular_function | DNA binding |
| C | 0003700 | molecular_function | DNA-binding transcription factor activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0006352 | biological_process | DNA-templated transcription initiation |
| C | 0006355 | biological_process | regulation of DNA-templated transcription |
| C | 0016987 | molecular_function | sigma factor activity |
| C | 2000142 | biological_process | regulation of DNA-templated transcription initiation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MG A 602 |
| Chain | Residue |
| A | ASN50 |
| A | HOH506 |
| A | ADP601 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE MG B 702 |
| Chain | Residue |
| B | ASN50 |
| B | ADP701 |
| site_id | AC3 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE ADP A 601 |
| Chain | Residue |
| A | ASP81 |
| A | ILE86 |
| A | ALA92 |
| A | PHE97 |
| A | THR98 |
| A | THR99 |
| A | ARG105 |
| A | SER106 |
| A | MET108 |
| A | GLY109 |
| A | PHE110 |
| A | THR130 |
| A | HOH502 |
| A | HOH506 |
| A | HOH528 |
| A | MG602 |
| A | ASN50 |
| A | ALA51 |
| A | HIS54 |
| A | GLY55 |
| site_id | AC4 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE ADP B 701 |
| Chain | Residue |
| B | ASN50 |
| B | ALA51 |
| B | HIS54 |
| B | GLY55 |
| B | ASP81 |
| B | GLY85 |
| B | ILE86 |
| B | ALA92 |
| B | PHE97 |
| B | THR98 |
| B | THR99 |
| B | GLY107 |
| B | MET108 |
| B | GLY109 |
| B | PHE110 |
| B | THR130 |
| B | HOH503 |
| B | HOH507 |
| B | HOH509 |
| B | HOH568 |
| B | MG702 |
Functional Information from PROSITE/UniProt
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | a catalytic site defined by CSA, PubMed 15236958, 11955433 |
| Chain | Residue | Details |
| A | GLU46 | |
| A | ARG105 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | a catalytic site defined by CSA, PubMed 15236958, 11955433 |
| Chain | Residue | Details |
| B | GLU46 | |
| B | ARG105 |
| site_id | MCSA1 |
| Number of Residues | 3 |
| Details | M-CSA 760 |
| Chain | Residue | Details |
| A | GLU46 | activator, electrostatic stabiliser, proton acceptor |
| A | ASN50 | metal ligand |
| A | ARG105 | electrostatic stabiliser, polar interaction |
| site_id | MCSA2 |
| Number of Residues | 3 |
| Details | M-CSA 760 |
| Chain | Residue | Details |
| B | GLU46 | activator, electrostatic stabiliser, proton acceptor |
| B | ASN50 | metal ligand |
| B | ARG105 | electrostatic stabiliser, polar interaction |
