1L0O

Crystal Structure of the Bacillus stearothermophilus Anti-Sigma Factor SpoIIAB with the Sporulation Sigma Factor SigmaF

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0004672	molecular_function	protein kinase activity
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0006468	biological_process	protein phosphorylation
A	0010468	biological_process	regulation of gene expression
A	0016301	molecular_function	kinase activity
A	0016740	molecular_function	transferase activity
A	0016989	molecular_function	sigma factor antagonist activity
A	0030435	biological_process	sporulation resulting in formation of a cellular spore
A	0030436	biological_process	asexual sporulation
A	0042174	biological_process	negative regulation of sporulation resulting in formation of a cellular spore
A	0045892	biological_process	negative regulation of DNA-templated transcription
A	0106310	molecular_function	protein serine kinase activity
B	0000166	molecular_function	nucleotide binding
B	0004672	molecular_function	protein kinase activity
B	0004674	molecular_function	protein serine/threonine kinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0006468	biological_process	protein phosphorylation
B	0010468	biological_process	regulation of gene expression
B	0016301	molecular_function	kinase activity
B	0016740	molecular_function	transferase activity
B	0016989	molecular_function	sigma factor antagonist activity
B	0030435	biological_process	sporulation resulting in formation of a cellular spore
B	0030436	biological_process	asexual sporulation
B	0042174	biological_process	negative regulation of sporulation resulting in formation of a cellular spore
B	0045892	biological_process	negative regulation of DNA-templated transcription
B	0106310	molecular_function	protein serine kinase activity
C	0003677	molecular_function	DNA binding
C	0003700	molecular_function	DNA-binding transcription factor activity
C	0005515	molecular_function	protein binding
C	0006352	biological_process	DNA-templated transcription initiation
C	0006355	biological_process	regulation of DNA-templated transcription
C	0016987	molecular_function	sigma factor activity
C	0030435	biological_process	sporulation resulting in formation of a cellular spore
C	2000142	biological_process	regulation of DNA-templated transcription initiation

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 602

Chain	Residue
A	ASN50
A	HOH506
A	ADP601

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site_id	AC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG B 702

Chain	Residue
B	ASN50
B	ADP701

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site_id	AC3
Number of Residues	20
Details	BINDING SITE FOR RESIDUE ADP A 601

Chain	Residue
A	ASP81
A	ILE86
A	ALA92
A	PHE97
A	THR98
A	THR99
A	ARG105
A	SER106
A	MET108
A	GLY109
A	PHE110
A	THR130
A	HOH502
A	HOH506
A	HOH528
A	MG602
A	ASN50
A	ALA51
A	HIS54
A	GLY55

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site_id	AC4
Number of Residues	21
Details	BINDING SITE FOR RESIDUE ADP B 701

Chain	Residue
B	ASN50
B	ALA51
B	HIS54
B	GLY55
B	ASP81
B	GLY85
B	ILE86
B	ALA92
B	PHE97
B	THR98
B	THR99
B	GLY107
B	MET108
B	GLY109
B	PHE110
B	THR130
B	HOH503
B	HOH507
B	HOH509
B	HOH568
B	MG702

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Functional Information from PROSITE/UniProt

site_id	PS00715
Number of Residues	14
Details	SIGMA70_1 Sigma-70 factors family signature 1. DLFQiGcIGLLkSV

Chain	Residue	Details
C	ASP59-VAL72

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site_id	PS00716
Number of Residues	27
Details	SIGMA70_2 Sigma-70 factors family signature 2. TqsEVAsrlgISqvqMsRLEkkiLqhI

Chain	Residue	Details
C	THR218-ILE244

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	a catalytic site defined by CSA, PubMed 15236958, 11955433

Chain	Residue	Details
A	GLU46
A	ARG105

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site_id	CSA2
Number of Residues	2
Details	a catalytic site defined by CSA, PubMed 15236958, 11955433

Chain	Residue	Details
B	GLU46
B	ARG105

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site_id	MCSA1
Number of Residues	3
Details	M-CSA 760

Chain	Residue	Details
A	GLU46	activator, electrostatic stabiliser, proton acceptor
A	ASN50	metal ligand
A	ARG105	electrostatic stabiliser, polar interaction

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site_id	MCSA2
Number of Residues	3
Details	M-CSA 760

Chain	Residue	Details
B	GLU46	activator, electrostatic stabiliser, proton acceptor
B	ASN50	metal ligand
B	ARG105	electrostatic stabiliser, polar interaction

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