1L0O
Crystal Structure of the Bacillus stearothermophilus Anti-Sigma Factor SpoIIAB with the Sporulation Sigma Factor SigmaF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2001-06-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 97.310, 97.310, 262.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.000 - 2.900 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.27900 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 0.288 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.030 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.081 * | 0.307 * |
| Total number of observations | 133526 * | |
| Number of reflections | 17312 | |
| <I/σ(I)> | 30.8 | 6.4 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 22.5 * | MES, ammonium sulfate, dioxane, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | ATP | 1 (mM) | |
| 5 | 1 | drop | TCEP | 1 (mM) | |
| 6 | 1 | drop | protein | 20 (mg/ml) | |
| 7 | 1 | reservoir | ammonium sulfate | 1.9 (M) | |
| 8 | 1 | reservoir | MES | 100 (mM) | pH6.0 |
| 9 | 1 | reservoir | dioxane | 10 (%(v/v)) |
