1HR9

Yeast Mitochondrial Processing Peptidase beta-E73Q Mutant Complexed with Malate Dehydrogenase Signal Peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, E, G)	MITOCHONDRIAL PROCESSING PEPTIDASE ALPHA SUBUNIT	polymer	475	52532.4	4	UniProt (P11914) Pfam (PF00675) Pfam (PF05193)	Saccharomyces cerevisiae (baker's yeast)	ALPHA-MPP
2	B, D, F, H (B, D, F, H)	MITOCHONDRIAL PROCESSING PEPTIDASE BETA SUBUNIT	polymer	443	48894.0	4	UniProt (P10507) Pfam (PF00675) Pfam (PF05193)	Saccharomyces cerevisiae (baker's yeast)	BETA-MPP
3	I, J, K, L (O, P, Q, R)	MALATE DEHYDROGENASE	polymer	8	903.1	4	UniProt (P17505)		MDH1
4	M, N, P, R (B, D, F, H)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	O, Q (E, G)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	2	Chemie (EPE)

Sequence modifications

A, C, E, G: 14 - 482 (UniProt: P11914)

PDB	External Database	Details
Gly 177	Glu 177	SEE REMARK 999
Gly 217	Glu 217	SEE REMARK 999
His 483	-	expression tag
His 484	-	expression tag
His 485	-	expression tag
His 486	-	expression tag
His 487	-	expression tag
His 488	-	expression tag

B, D, F, H: 21 - 462 (UniProt: P10507)

PDB	External Database	Details
Ala 20	-	cloning artifact
Gln 73	Glu 73	engineered mutation
Pro 84	Ser 84	SEE REMARK 999
Arg 350	Gln 350	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	409318.1
Non-Polymers*	Number of molecules	6
	Total formula weight	738.2
All*	Total formula weight	410056.4

*Water molecules are not included.