1HR9
Yeast Mitochondrial Processing Peptidase beta-E73Q Mutant Complexed with Malate Dehydrogenase Signal Peptide
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004222 | molecular_function | metalloendopeptidase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005759 | cellular_component | mitochondrial matrix |
| A | 0006508 | biological_process | proteolysis |
| A | 0006627 | biological_process | protein processing involved in protein targeting to mitochondrion |
| A | 0017087 | cellular_component | mitochondrial processing peptidase complex |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0061133 | molecular_function | endopeptidase activator activity |
| B | 0004222 | molecular_function | metalloendopeptidase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005759 | cellular_component | mitochondrial matrix |
| B | 0006508 | biological_process | proteolysis |
| B | 0006627 | biological_process | protein processing involved in protein targeting to mitochondrion |
| B | 0008237 | molecular_function | metallopeptidase activity |
| B | 0008270 | molecular_function | zinc ion binding |
| B | 0017087 | cellular_component | mitochondrial processing peptidase complex |
| B | 0046872 | molecular_function | metal ion binding |
| C | 0004222 | molecular_function | metalloendopeptidase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005739 | cellular_component | mitochondrion |
| C | 0005759 | cellular_component | mitochondrial matrix |
| C | 0006508 | biological_process | proteolysis |
| C | 0006627 | biological_process | protein processing involved in protein targeting to mitochondrion |
| C | 0017087 | cellular_component | mitochondrial processing peptidase complex |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0061133 | molecular_function | endopeptidase activator activity |
| D | 0004222 | molecular_function | metalloendopeptidase activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005739 | cellular_component | mitochondrion |
| D | 0005759 | cellular_component | mitochondrial matrix |
| D | 0006508 | biological_process | proteolysis |
| D | 0006627 | biological_process | protein processing involved in protein targeting to mitochondrion |
| D | 0008237 | molecular_function | metallopeptidase activity |
| D | 0008270 | molecular_function | zinc ion binding |
| D | 0017087 | cellular_component | mitochondrial processing peptidase complex |
| D | 0046872 | molecular_function | metal ion binding |
| E | 0004222 | molecular_function | metalloendopeptidase activity |
| E | 0005515 | molecular_function | protein binding |
| E | 0005739 | cellular_component | mitochondrion |
| E | 0005759 | cellular_component | mitochondrial matrix |
| E | 0006508 | biological_process | proteolysis |
| E | 0006627 | biological_process | protein processing involved in protein targeting to mitochondrion |
| E | 0017087 | cellular_component | mitochondrial processing peptidase complex |
| E | 0046872 | molecular_function | metal ion binding |
| E | 0061133 | molecular_function | endopeptidase activator activity |
| F | 0004222 | molecular_function | metalloendopeptidase activity |
| F | 0005515 | molecular_function | protein binding |
| F | 0005739 | cellular_component | mitochondrion |
| F | 0005759 | cellular_component | mitochondrial matrix |
| F | 0006508 | biological_process | proteolysis |
| F | 0006627 | biological_process | protein processing involved in protein targeting to mitochondrion |
| F | 0008237 | molecular_function | metallopeptidase activity |
| F | 0008270 | molecular_function | zinc ion binding |
| F | 0017087 | cellular_component | mitochondrial processing peptidase complex |
| F | 0046872 | molecular_function | metal ion binding |
| G | 0004222 | molecular_function | metalloendopeptidase activity |
| G | 0005515 | molecular_function | protein binding |
| G | 0005739 | cellular_component | mitochondrion |
| G | 0005759 | cellular_component | mitochondrial matrix |
| G | 0006508 | biological_process | proteolysis |
| G | 0006627 | biological_process | protein processing involved in protein targeting to mitochondrion |
| G | 0017087 | cellular_component | mitochondrial processing peptidase complex |
| G | 0046872 | molecular_function | metal ion binding |
| G | 0061133 | molecular_function | endopeptidase activator activity |
| H | 0004222 | molecular_function | metalloendopeptidase activity |
| H | 0005515 | molecular_function | protein binding |
| H | 0005739 | cellular_component | mitochondrion |
| H | 0005759 | cellular_component | mitochondrial matrix |
| H | 0006508 | biological_process | proteolysis |
| H | 0006627 | biological_process | protein processing involved in protein targeting to mitochondrion |
| H | 0008237 | molecular_function | metallopeptidase activity |
| H | 0008270 | molecular_function | zinc ion binding |
| H | 0017087 | cellular_component | mitochondrial processing peptidase complex |
| H | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B 501 |
| Chain | Residue |
| B | HIS70 |
| B | HIS74 |
| B | GLU150 |
| O | ALA9 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN D 502 |
| Chain | Residue |
| D | HIS70 |
| D | HIS74 |
| D | GLU150 |
| P | ALA9 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN F 503 |
| Chain | Residue |
| F | HIS74 |
| F | GLU150 |
| Q | ALA9 |
| F | HIS70 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN H 504 |
| Chain | Residue |
| H | HIS70 |
| H | HIS74 |
| H | GLU150 |
| R | ALA9 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE EPE G 489 |
| Chain | Residue |
| C | TRP147 |
| C | PRO175 |
| C | ARG176 |
| G | PRO175 |
| G | ARG176 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE EPE E 489 |
| Chain | Residue |
| A | PRO175 |
| A | ARG176 |
| E | PRO175 |
| E | ARG176 |
Functional Information from PROSITE/UniProt
| site_id | PS00143 |
| Number of Residues | 24 |
| Details | INSULINASE Insulinase family, zinc-binding region signature. GsrfegrnlkGcTHILDRLaFkST |
| Chain | Residue | Details |
| A | GLY49-THR72 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor => ECO:0000269|PubMed:11470436, ECO:0007744|PDB:1HR6 |
| Chain | Residue | Details |
| B | GLN73 | |
| D | GLN73 | |
| F | GLN73 | |
| H | GLN73 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU10096, ECO:0000269|PubMed:11470436, ECO:0000269|PubMed:9654444, ECO:0007744|PDB:1HR6, ECO:0007744|PDB:1HR7, ECO:0007744|PDB:1HR8, ECO:0007744|PDB:1HR9 |
| Chain | Residue | Details |
| B | HIS70 | |
| D | HIS70 | |
| F | HIS70 | |
| H | HIS70 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU10096, ECO:0000269|PubMed:11470436, ECO:0007744|PDB:1HR6, ECO:0007744|PDB:1HR7, ECO:0007744|PDB:1HR8, ECO:0007744|PDB:1HR9 |
| Chain | Residue | Details |
| B | HIS74 | |
| B | GLU150 | |
| D | HIS74 | |
| D | GLU150 | |
| F | HIS74 | |
| F | GLU150 | |
| H | HIS74 | |
| H | GLU150 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956 |
| Chain | Residue | Details |
| B | SER243 | |
| D | SER243 | |
| F | SER243 | |
| H | SER243 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1hr6 |
| Chain | Residue | Details |
| B | GLN73 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1hr6 |
| Chain | Residue | Details |
| D | GLN73 |
| site_id | CSA3 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1hr6 |
| Chain | Residue | Details |
| F | GLN73 |
| site_id | CSA4 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1hr6 |
| Chain | Residue | Details |
| H | GLN73 |
| site_id | CSA5 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1hr6 |
| Chain | Residue | Details |
| A | ASP65 |
| site_id | CSA6 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1hr6 |
| Chain | Residue | Details |
| C | ASP65 |
| site_id | CSA7 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1hr6 |
| Chain | Residue | Details |
| E | ASP65 |
| site_id | CSA8 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1hr6 |
| Chain | Residue | Details |
| G | ASP65 |
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 657 |
| Chain | Residue | Details |
| B | HIS70 | metal ligand |
| B | GLN73 | proton acceptor, proton donor |
| B | HIS74 | hydrogen bond donor, metal ligand |
| B | GLU143 | electrostatic stabiliser, hydrogen bond acceptor |
| B | GLU150 | metal ligand |
| site_id | MCSA2 |
| Number of Residues | 5 |
| Details | M-CSA 657 |
| Chain | Residue | Details |
| D | HIS70 | metal ligand |
| D | GLN73 | proton acceptor, proton donor |
| D | HIS74 | hydrogen bond donor, metal ligand |
| D | GLU143 | electrostatic stabiliser, hydrogen bond acceptor |
| D | GLU150 | metal ligand |
| site_id | MCSA3 |
| Number of Residues | 5 |
| Details | M-CSA 657 |
| Chain | Residue | Details |
| F | HIS70 | metal ligand |
| F | GLN73 | proton acceptor, proton donor |
| F | HIS74 | hydrogen bond donor, metal ligand |
| F | GLU143 | electrostatic stabiliser, hydrogen bond acceptor |
| F | GLU150 | metal ligand |
| site_id | MCSA4 |
| Number of Residues | 5 |
| Details | M-CSA 657 |
| Chain | Residue | Details |
| H | HIS70 | metal ligand |
| H | GLN73 | proton acceptor, proton donor |
| H | HIS74 | hydrogen bond donor, metal ligand |
| H | GLU143 | electrostatic stabiliser, hydrogen bond acceptor |
| H | GLU150 | metal ligand |
