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1HR9

Yeast Mitochondrial Processing Peptidase beta-E73Q Mutant Complexed with Malate Dehydrogenase Signal Peptide

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsCHESS BEAMLINE F1
Synchrotron siteCHESS
BeamlineF1
Temperature [K]105
Detector technologyCCD
Collection date2000-06-30
DetectorADSC QUANTUM 4
Wavelength(s)0.9430
Spacegroup nameP 21 21 21
Unit cell lengths134.370, 178.520, 201.950
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution47.550 - 3.010
R-factor0.217
Rwork0.217
R-free0.25600
Starting model (for MR)PDB ID 1HR6
RMSD bond length0.014
RMSD bond angle1.600
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0003.110
High resolution limit [Å]3.0003.000
Rmerge0.0940.481
Total number of observations418644

*

Number of reflections90666
<I/σ(I)>13.62.3
Completeness [%]93.595.9
Redundancy4.64.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

*

7.5

*

21

*

Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein8-14 (mg/ml)
101reservoirn-dodecylmaltoside0.2 (mM)
111reservoirsodium azide2 (mM)
21dropHEPES50 (mM)
31dropglycerol30 (%(v/v))
41dropn-dodecylmaltoside0.2 (mM)
51reservoirPEG100005-9 (%(w/v))
61reservoirethylene glycol35 (%(v/v))
71reservoirMPD6 (%(v/v))
81reservoirsodium citrate25 (mM)
91reservoirbenzamidine2 (%(w/v))

227111

PDB entries from 2024-11-06

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