1G6I
Crystal structure of the yeast alpha-1,2-mannosidase with bound 1-deoxymannojirimycin at 1.59 A resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CLASS I ALPHA-1,2-MANNOSIDASE | polymer | 545 | 62632.5 | 1 | UniProt (P32906) Pfam (PF01532) | Saccharomyces cerevisiae (yeast) | |
| 2 | B (B) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 1 | In PDB GlyTouCan (G34725OX) | |||
| 3 | C (C) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 4 | D (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
| 5 | E (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 6 | F (A) | 1-DEOXYMANNOJIRIMYCIN | non-polymer | 163.2 | 1 | Chemie (DMJ) | |||
| 7 | G (A) | water | water | 18.0 | 415 | Chemie (HOH) |
Sequence modifications
A: 0 - 549 (UniProt: P32906)
| PDB | External Database | Details |
|---|---|---|
| Asp 38 | - | insertion |
| Gln 328 | His 328 | conflict |
| - | Leu 367 | deletion |
| - | Ser 368 | deletion |
| - | Leu 369 | deletion |
| - | Glu 370 | deletion |
| - | Arg 371 | deletion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 62632.5 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 2145.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 424.5 | |
| All* | Total formula weight | 65202.9 |
