1G6I
Crystal structure of the yeast alpha-1,2-mannosidase with bound 1-deoxymannojirimycin at 1.59 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 1999-11-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 89.052, 89.052, 153.142 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.560 - 1.590 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.25300 |
| Structure solution method | AB INITIO PHASING |
| Starting model (for MR) | Ab initio |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 1.690 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.056 | 0.410 |
| Number of reflections | 590345 | |
| <I/σ(I)> | 9.9 | 3.5 |
| Completeness [%] | 93.5 | 85.9 |
| Redundancy | 6.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | PEG MME 2K, Sodium Citrate, Ammonium Acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
