1G6I
Crystal structure of the yeast alpha-1,2-mannosidase with bound 1-deoxymannojirimycin at 1.59 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 1999-11-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 89.052, 89.052, 153.142 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.560 - 1.590 |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.25300 |
Structure solution method | AB INITIO PHASING |
Starting model (for MR) | Ab initio |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | 1.690 |
High resolution limit [Å] | 1.590 | 1.590 |
Rmerge | 0.056 | 0.410 |
Number of reflections | 590345 | |
<I/σ(I)> | 9.9 | 3.5 |
Completeness [%] | 93.5 | 85.9 |
Redundancy | 6.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | PEG MME 2K, Sodium Citrate, Ammonium Acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |