1FNT

CRYSTAL STRUCTURE OF THE 20S PROTEASOME FROM YEAST IN COMPLEX WITH THE PROTEASOME ACTIVATOR PA26 FROM TRYPANOSOME BRUCEI AT 3.2 ANGSTROMS RESOLUTION

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, O (A, O)	PROTEASOME COMPONENT C7-ALPHA	polymer	252	28033.8	2	UniProt (P21243) Pfam (PF10584) Pfam (PF00227)	Saccharomyces cerevisiae (baker's yeast)
2	B, P (B, P)	PROTEASOME COMPONENT Y7	polymer	250	27191.8	2	UniProt (P23639) Pfam (PF10584) Pfam (PF00227)	Saccharomyces cerevisiae (baker's yeast)
3	C, Q (C, Q)	PROTEASOME COMPONENT Y13	polymer	245	27181.6	2	UniProt (P23638) Pfam (PF10584) Pfam (PF00227)	Saccharomyces cerevisiae (baker's yeast)
4	D, R (D, R)	PROTEASOME COMPONENT PRE6	polymer	254	28478.1	2	UniProt (P40303) Pfam (PF10584) Pfam (PF00227)	Saccharomyces cerevisiae (baker's yeast)
5	E, S (E, S)	PROTEASOME COMPONENT PUP2	polymer	260	28649.1	2	UniProt (P32379) Pfam (PF00227) Pfam (PF10584)	Saccharomyces cerevisiae (baker's yeast)
6	F, T (F, T)	PROTEASOME COMPONENT PRE5	polymer	234	25634.0	2	UniProt (P40302) Pfam (PF10584) Pfam (PF00227)	Saccharomyces cerevisiae (baker's yeast)
7	G, U (G, U)	PROTEASOME COMPONENT C1	polymer	287	31443.9	2	UniProt (P21242) Pfam (PF10584) Pfam (PF00227)	Saccharomyces cerevisiae (baker's yeast)
8	H, V (H, V)	PROTEASOME COMPONENT PRE3	polymer	196	21517.2	2	UniProt (P38624) Pfam (PF00227)	Saccharomyces cerevisiae (baker's yeast)
9	I, W (I, W)	PROTEASOME COMPONENT PUP1	polymer	232	25114.5	2	UniProt (P25043) Pfam (PF00227) Pfam (PF12465)	Saccharomyces cerevisiae (baker's yeast)
10	J, X (J, X)	PROTEASOME COMPONENT PUP3	polymer	205	22627.8	2	UniProt (P25451) Pfam (PF00227)	Saccharomyces cerevisiae (baker's yeast)
11	K, Y (K, Y)	PROTEASOME COMPONENT C11	polymer	198	22545.7	2	UniProt (P22141) Pfam (PF00227)	Saccharomyces cerevisiae (baker's yeast)
12	L, Z (L, Z)	PROTEASOME COMPONENT PRE2	polymer	212	23353.3	2	UniProt (P30656) Pfam (PF00227)	Saccharomyces cerevisiae (baker's yeast)
13	AA, M (M, a)	PROTEASOME COMPONENT C5	polymer	222	24883.9	2	UniProt (P23724) Pfam (PF00227)	Saccharomyces cerevisiae (baker's yeast)
14	BA, N (N, b)	PROTEASOME COMPONENT PRE4	polymer	233	25945.5	2	UniProt (P30657) Pfam (PF00227)	Saccharomyces cerevisiae (baker's yeast)
15	CA, DA, EA, FA, GA... (c, d, e, f, g...)	PROTEASOME ACTIVATOR PROTEIN PA26	polymer	231	25272.8	14	UniProt (Q9U8G2) Pfam (PF02252)	Trypanosoma brucei
16	AB, BB, CB, DB, QA... (X, Z, a, G, H...)	MAGNESIUM ION	non-polymer		24.3	14	Chemie (MG)

Sequence modifications

L, Z: 1 - 212 (UniProt: P30656)

PDB	External Database	Details
Arg 33	Lys 108	engineered mutation

c, d, e, f, g, h, i, j, k, l, m, n, o, p: 1 - 231 (UniProt: Q9U8G2)

PDB	External Database	Details
Val 49	Thr 49	see remark 999
Thr 226	Ser 226	see remark 999

Contents of the asymmetric unit

Polymers	Number of chains	42
	Total formula weight	1079019.4
Non-Polymers*	Number of molecules	14
	Total formula weight	340.3
All*	Total formula weight	1079359.7

*Water molecules are not included.