1FNT

CRYSTAL STRUCTURE OF THE 20S PROTEASOME FROM YEAST IN COMPLEX WITH THE PROTEASOME ACTIVATOR PA26 FROM TRYPANOSOME BRUCEI AT 3.2 ANGSTROMS RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-02-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	192.964, 232.127, 296.772
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.200
R-factor	0.255
Rwork	0.255
R-free	0.32500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1/2 OF 20S PROTEASOME INCLUDING ALPHA-1-7 AND REMARK 200 BETA-1-7 REDUCED TO A POLY-ALANINE MODEL.
RMSD bond length	0.013
RMSD bond angle	23.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Rmerge	0.112	0.331
Total number of observations	503540 *
Number of reflections	189495	6632 *
<I/σ(I)>	7	1.9
Completeness [%]	89.1	62.9
Redundancy	2.7	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.1 M NaHEPES, 40% MPD, 0.2 M NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	10 (mM)
2	1	drop	EDTA	1 (mM)
3	1	drop	dithiothreitol	1 (mM)
4	1	drop	protein	10 (mg/ml)
5	1	reservoir	sodium HEPES	0.1 (M)
6	1	reservoir	MPD	40 (%)
7	1	reservoir		0.2 (M)