9YXQ

Cryo-EM structure of the Xenopus laevis mitotic centromere-associated kinesin (MCAK) bound to the paclitaxel-stabilized microtubule

Functional Information from GO Data

Chain	GOid	namespace	contents
1	0000226	biological_process	microtubule cytoskeleton organization
1	0000278	biological_process	mitotic cell cycle
1	0003924	molecular_function	GTPase activity
1	0005200	molecular_function	structural constituent of cytoskeleton
1	0005525	molecular_function	GTP binding
1	0005856	cellular_component	cytoskeleton
1	0015630	cellular_component	microtubule cytoskeleton
1	0030182	biological_process	neuron differentiation
2	0000278	biological_process	mitotic cell cycle
2	0001764	biological_process	neuron migration
2	0005200	molecular_function	structural constituent of cytoskeleton
2	0005515	molecular_function	protein binding
2	0005525	molecular_function	GTP binding
2	0005856	cellular_component	cytoskeleton
2	0007010	biological_process	cytoskeleton organization
3	0000226	biological_process	microtubule cytoskeleton organization
3	0000278	biological_process	mitotic cell cycle
3	0003924	molecular_function	GTPase activity
3	0005200	molecular_function	structural constituent of cytoskeleton
3	0005525	molecular_function	GTP binding
3	0005856	cellular_component	cytoskeleton
3	0015630	cellular_component	microtubule cytoskeleton
3	0030182	biological_process	neuron differentiation
4	0000278	biological_process	mitotic cell cycle
4	0001764	biological_process	neuron migration
4	0005200	molecular_function	structural constituent of cytoskeleton
4	0005515	molecular_function	protein binding
4	0005525	molecular_function	GTP binding
4	0005856	cellular_component	cytoskeleton
4	0007010	biological_process	cytoskeleton organization
5	0000278	biological_process	mitotic cell cycle
5	0001764	biological_process	neuron migration
5	0005200	molecular_function	structural constituent of cytoskeleton
5	0005515	molecular_function	protein binding
5	0005525	molecular_function	GTP binding
5	0005856	cellular_component	cytoskeleton
5	0007010	biological_process	cytoskeleton organization
6	0000226	biological_process	microtubule cytoskeleton organization
6	0000278	biological_process	mitotic cell cycle
6	0003924	molecular_function	GTPase activity
6	0005200	molecular_function	structural constituent of cytoskeleton
6	0005525	molecular_function	GTP binding
6	0005856	cellular_component	cytoskeleton
6	0015630	cellular_component	microtubule cytoskeleton
6	0030182	biological_process	neuron differentiation
7	0000278	biological_process	mitotic cell cycle
7	0001764	biological_process	neuron migration
7	0005200	molecular_function	structural constituent of cytoskeleton
7	0005515	molecular_function	protein binding
7	0005525	molecular_function	GTP binding
7	0005856	cellular_component	cytoskeleton
7	0007010	biological_process	cytoskeleton organization
8	0000226	biological_process	microtubule cytoskeleton organization
8	0000278	biological_process	mitotic cell cycle
8	0003924	molecular_function	GTPase activity
8	0005200	molecular_function	structural constituent of cytoskeleton
8	0005525	molecular_function	GTP binding
8	0005856	cellular_component	cytoskeleton
8	0015630	cellular_component	microtubule cytoskeleton
8	0030182	biological_process	neuron differentiation
a	0000226	biological_process	microtubule cytoskeleton organization
a	0000278	biological_process	mitotic cell cycle
a	0003924	molecular_function	GTPase activity
a	0005200	molecular_function	structural constituent of cytoskeleton
a	0005525	molecular_function	GTP binding
a	0005856	cellular_component	cytoskeleton
a	0015630	cellular_component	microtubule cytoskeleton
a	0030182	biological_process	neuron differentiation
A	0000226	biological_process	microtubule cytoskeleton organization
A	0000278	biological_process	mitotic cell cycle
A	0003924	molecular_function	GTPase activity
A	0005200	molecular_function	structural constituent of cytoskeleton
A	0005525	molecular_function	GTP binding
A	0005856	cellular_component	cytoskeleton
A	0015630	cellular_component	microtubule cytoskeleton
A	0030182	biological_process	neuron differentiation
b	0000278	biological_process	mitotic cell cycle
b	0001764	biological_process	neuron migration
b	0005200	molecular_function	structural constituent of cytoskeleton
b	0005515	molecular_function	protein binding
b	0005525	molecular_function	GTP binding
b	0005856	cellular_component	cytoskeleton
b	0007010	biological_process	cytoskeleton organization
B	0000278	biological_process	mitotic cell cycle
B	0001764	biological_process	neuron migration
B	0005200	molecular_function	structural constituent of cytoskeleton
B	0005515	molecular_function	protein binding
B	0005525	molecular_function	GTP binding
B	0005856	cellular_component	cytoskeleton
B	0007010	biological_process	cytoskeleton organization
m	0000775	cellular_component	chromosome, centromeric region
m	0000776	cellular_component	kinetochore
m	0003777	molecular_function	microtubule motor activity
m	0005634	cellular_component	nucleus
m	0005856	cellular_component	cytoskeleton
m	0005874	cellular_component	microtubule
m	0005881	cellular_component	cytoplasmic microtubule
m	0007019	biological_process	microtubule depolymerization
m	0035371	cellular_component	microtubule plus-end
m	0051310	biological_process	metaphase chromosome alignment
m	0051315	biological_process	attachment of mitotic spindle microtubules to kinetochore
M	0000775	cellular_component	chromosome, centromeric region
M	0000776	cellular_component	kinetochore
M	0003777	molecular_function	microtubule motor activity
M	0005634	cellular_component	nucleus
M	0005856	cellular_component	cytoskeleton
M	0005874	cellular_component	microtubule
M	0005881	cellular_component	cytoplasmic microtubule
M	0007019	biological_process	microtubule depolymerization
M	0035371	cellular_component	microtubule plus-end
M	0051310	biological_process	metaphase chromosome alignment
M	0051315	biological_process	attachment of mitotic spindle microtubules to kinetochore

Functional Information from PROSITE/UniProt

site_id	PS00227
Number of Residues	7
Details	TUBULIN Tubulin subunits alpha, beta, and gamma signature. GGGTGSG

Chain	Residue	Details
2	GLY142-GLY148
1	GLY142-GLY148

---

site_id	PS00228
Number of Residues	4
Details	TUBULIN_B_AUTOREG Tubulin-beta mRNA autoregulation signal. MREI

Chain	Residue	Details
2	MET1-ILE4

---

site_id	PS00411
Number of Residues	12
Details	KINESIN_MOTOR_1 Kinesin motor domain signature. GKFsLVDLAGNE

Chain	Residue	Details
M	GLY490-GLU501

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	18
Details	Motif: {"description":"MREC motif","evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	Active site: {"evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	108
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	Modified residue: {"description":"3'-nitrotyrosine","evidences":[{"source":"UniProtKB","id":"P68373","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	Modified residue: {"description":"Omega-N-methylarginine","evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	18
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	18
Details	Motif: {"description":"MREI motif","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	42
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q13509","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P99024","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	6
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P99024","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine; by CDK1","evidences":[{"source":"UniProtKB","id":"Q13885","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	12
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	6
Details	Modified residue: {"description":"Omega-N-methylarginine","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	6
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	12
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	62
Details	Region: {"description":"Negative regulator of microtubule-binding","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	2
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00283","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---