9MJ4

Yeast V-ATPase Vo proton channel bound to nanobody 2WVA149

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
A	0000324	cellular_component	fungal-type vacuole
A	0000329	cellular_component	fungal-type vacuole membrane
A	0005515	molecular_function	protein binding
A	0005774	cellular_component	vacuolar membrane
A	0006797	biological_process	polyphosphate metabolic process
A	0006811	biological_process	monoatomic ion transport
A	0007035	biological_process	vacuolar acidification
A	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
A	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
A	0045121	cellular_component	membrane raft
A	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
A	0051117	molecular_function	ATPase binding
A	0065003	biological_process	protein-containing complex assembly
A	0071469	biological_process	cellular response to alkaline pH
A	0071474	biological_process	cellular hyperosmotic response
A	0080025	molecular_function	phosphatidylinositol-3,5-bisphosphate binding
A	1902600	biological_process	proton transmembrane transport
A	1903778	biological_process	protein localization to vacuolar membrane
B	0000139	cellular_component	Golgi membrane
B	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
B	0000329	cellular_component	fungal-type vacuole membrane
B	0005515	molecular_function	protein binding
B	0005774	cellular_component	vacuolar membrane
B	0006811	biological_process	monoatomic ion transport
B	0007034	biological_process	vacuolar transport
B	0007035	biological_process	vacuolar acidification
B	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
B	0033176	cellular_component	proton-transporting V-type ATPase complex
B	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
B	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
B	0048388	biological_process	endosomal lumen acidification
B	0061795	biological_process	Golgi lumen acidification
B	1902600	biological_process	proton transmembrane transport
C	0000139	cellular_component	Golgi membrane
C	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
C	0000329	cellular_component	fungal-type vacuole membrane
C	0005774	cellular_component	vacuolar membrane
C	0006811	biological_process	monoatomic ion transport
C	0007035	biological_process	vacuolar acidification
C	0008553	molecular_function	P-type proton-exporting transporter activity
C	0015078	molecular_function	proton transmembrane transporter activity
C	0016020	cellular_component	membrane
C	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
C	0033176	cellular_component	proton-transporting V-type ATPase complex
C	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
C	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
C	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
C	0048388	biological_process	endosomal lumen acidification
C	0061795	biological_process	Golgi lumen acidification
C	1902600	biological_process	proton transmembrane transport
D	0000139	cellular_component	Golgi membrane
D	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
D	0000324	cellular_component	fungal-type vacuole
D	0000329	cellular_component	fungal-type vacuole membrane
D	0005774	cellular_component	vacuolar membrane
D	0006811	biological_process	monoatomic ion transport
D	0007035	biological_process	vacuolar acidification
D	0015078	molecular_function	proton transmembrane transporter activity
D	0016020	cellular_component	membrane
D	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
D	0033176	cellular_component	proton-transporting V-type ATPase complex
D	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
D	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
D	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
D	0048388	biological_process	endosomal lumen acidification
D	0061795	biological_process	Golgi lumen acidification
D	0071944	cellular_component	cell periphery
D	1902600	biological_process	proton transmembrane transport
E	0000139	cellular_component	Golgi membrane
E	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
E	0000329	cellular_component	fungal-type vacuole membrane
E	0005774	cellular_component	vacuolar membrane
E	0006623	biological_process	protein targeting to vacuole
E	0006811	biological_process	monoatomic ion transport
E	0006878	biological_process	intracellular copper ion homeostasis
E	0006879	biological_process	intracellular iron ion homeostasis
E	0006897	biological_process	endocytosis
E	0007033	biological_process	vacuole organization
E	0007035	biological_process	vacuolar acidification
E	0015078	molecular_function	proton transmembrane transporter activity
E	0016020	cellular_component	membrane
E	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
E	0033176	cellular_component	proton-transporting V-type ATPase complex
E	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
E	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
E	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
E	0048388	biological_process	endosomal lumen acidification
E	0061795	biological_process	Golgi lumen acidification
E	1902600	biological_process	proton transmembrane transport
F	0000139	cellular_component	Golgi membrane
F	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
F	0000329	cellular_component	fungal-type vacuole membrane
F	0005774	cellular_component	vacuolar membrane
F	0006623	biological_process	protein targeting to vacuole
F	0006811	biological_process	monoatomic ion transport
F	0006878	biological_process	intracellular copper ion homeostasis
F	0006879	biological_process	intracellular iron ion homeostasis
F	0006897	biological_process	endocytosis
F	0007033	biological_process	vacuole organization
F	0007035	biological_process	vacuolar acidification
F	0015078	molecular_function	proton transmembrane transporter activity
F	0016020	cellular_component	membrane
F	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
F	0033176	cellular_component	proton-transporting V-type ATPase complex
F	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
F	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
F	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
F	0048388	biological_process	endosomal lumen acidification
F	0061795	biological_process	Golgi lumen acidification
F	1902600	biological_process	proton transmembrane transport
G	0000139	cellular_component	Golgi membrane
G	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
G	0000329	cellular_component	fungal-type vacuole membrane
G	0005774	cellular_component	vacuolar membrane
G	0006623	biological_process	protein targeting to vacuole
G	0006811	biological_process	monoatomic ion transport
G	0006878	biological_process	intracellular copper ion homeostasis
G	0006879	biological_process	intracellular iron ion homeostasis
G	0006897	biological_process	endocytosis
G	0007033	biological_process	vacuole organization
G	0007035	biological_process	vacuolar acidification
G	0015078	molecular_function	proton transmembrane transporter activity
G	0016020	cellular_component	membrane
G	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
G	0033176	cellular_component	proton-transporting V-type ATPase complex
G	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
G	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
G	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
G	0048388	biological_process	endosomal lumen acidification
G	0061795	biological_process	Golgi lumen acidification
G	1902600	biological_process	proton transmembrane transport
H	0000139	cellular_component	Golgi membrane
H	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
H	0000329	cellular_component	fungal-type vacuole membrane
H	0005774	cellular_component	vacuolar membrane
H	0006623	biological_process	protein targeting to vacuole
H	0006811	biological_process	monoatomic ion transport
H	0006878	biological_process	intracellular copper ion homeostasis
H	0006879	biological_process	intracellular iron ion homeostasis
H	0006897	biological_process	endocytosis
H	0007033	biological_process	vacuole organization
H	0007035	biological_process	vacuolar acidification
H	0015078	molecular_function	proton transmembrane transporter activity
H	0016020	cellular_component	membrane
H	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
H	0033176	cellular_component	proton-transporting V-type ATPase complex
H	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
H	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
H	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
H	0048388	biological_process	endosomal lumen acidification
H	0061795	biological_process	Golgi lumen acidification
H	1902600	biological_process	proton transmembrane transport
I	0000139	cellular_component	Golgi membrane
I	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
I	0000329	cellular_component	fungal-type vacuole membrane
I	0005774	cellular_component	vacuolar membrane
I	0006623	biological_process	protein targeting to vacuole
I	0006811	biological_process	monoatomic ion transport
I	0006878	biological_process	intracellular copper ion homeostasis
I	0006879	biological_process	intracellular iron ion homeostasis
I	0006897	biological_process	endocytosis
I	0007033	biological_process	vacuole organization
I	0007035	biological_process	vacuolar acidification
I	0015078	molecular_function	proton transmembrane transporter activity
I	0016020	cellular_component	membrane
I	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
I	0033176	cellular_component	proton-transporting V-type ATPase complex
I	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
I	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
I	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
I	0048388	biological_process	endosomal lumen acidification
I	0061795	biological_process	Golgi lumen acidification
I	1902600	biological_process	proton transmembrane transport
J	0000139	cellular_component	Golgi membrane
J	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
J	0000329	cellular_component	fungal-type vacuole membrane
J	0005774	cellular_component	vacuolar membrane
J	0006623	biological_process	protein targeting to vacuole
J	0006811	biological_process	monoatomic ion transport
J	0006878	biological_process	intracellular copper ion homeostasis
J	0006879	biological_process	intracellular iron ion homeostasis
J	0006897	biological_process	endocytosis
J	0007033	biological_process	vacuole organization
J	0007035	biological_process	vacuolar acidification
J	0015078	molecular_function	proton transmembrane transporter activity
J	0016020	cellular_component	membrane
J	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
J	0033176	cellular_component	proton-transporting V-type ATPase complex
J	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
J	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
J	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
J	0048388	biological_process	endosomal lumen acidification
J	0061795	biological_process	Golgi lumen acidification
J	1902600	biological_process	proton transmembrane transport
K	0000139	cellular_component	Golgi membrane
K	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
K	0000329	cellular_component	fungal-type vacuole membrane
K	0005774	cellular_component	vacuolar membrane
K	0006623	biological_process	protein targeting to vacuole
K	0006811	biological_process	monoatomic ion transport
K	0006878	biological_process	intracellular copper ion homeostasis
K	0006879	biological_process	intracellular iron ion homeostasis
K	0006897	biological_process	endocytosis
K	0007033	biological_process	vacuole organization
K	0007035	biological_process	vacuolar acidification
K	0015078	molecular_function	proton transmembrane transporter activity
K	0016020	cellular_component	membrane
K	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
K	0033176	cellular_component	proton-transporting V-type ATPase complex
K	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
K	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
K	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
K	0048388	biological_process	endosomal lumen acidification
K	0061795	biological_process	Golgi lumen acidification
K	1902600	biological_process	proton transmembrane transport
L	0000139	cellular_component	Golgi membrane
L	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
L	0000329	cellular_component	fungal-type vacuole membrane
L	0005774	cellular_component	vacuolar membrane
L	0006623	biological_process	protein targeting to vacuole
L	0006811	biological_process	monoatomic ion transport
L	0006878	biological_process	intracellular copper ion homeostasis
L	0006879	biological_process	intracellular iron ion homeostasis
L	0006897	biological_process	endocytosis
L	0007033	biological_process	vacuole organization
L	0007035	biological_process	vacuolar acidification
L	0015078	molecular_function	proton transmembrane transporter activity
L	0016020	cellular_component	membrane
L	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
L	0033176	cellular_component	proton-transporting V-type ATPase complex
L	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
L	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
L	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
L	0048388	biological_process	endosomal lumen acidification
L	0061795	biological_process	Golgi lumen acidification
L	1902600	biological_process	proton transmembrane transport
M	0000032	biological_process	cell wall mannoprotein biosynthetic process
M	0000139	cellular_component	Golgi membrane
M	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
M	0000329	cellular_component	fungal-type vacuole membrane
M	0005774	cellular_component	vacuolar membrane
M	0006811	biological_process	monoatomic ion transport
M	0007035	biological_process	vacuolar acidification
M	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
M	0033176	cellular_component	proton-transporting V-type ATPase complex
M	0033179	cellular_component	proton-transporting V-type ATPase, V0 domain
M	0042625	molecular_function	ATPase-coupled ion transmembrane transporter activity
M	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
M	0048388	biological_process	endosomal lumen acidification
M	0055085	biological_process	transmembrane transport
M	0061795	biological_process	Golgi lumen acidification
M	0065003	biological_process	protein-containing complex assembly
M	1902600	biological_process	proton transmembrane transport
O	0000139	cellular_component	Golgi membrane
O	0000220	cellular_component	vacuolar proton-transporting V-type ATPase, V0 domain
O	0000329	cellular_component	fungal-type vacuole membrane
O	0003674	molecular_function	molecular_function
O	0004521	molecular_function	RNA endonuclease activity
O	0005575	cellular_component	cellular_component
O	0005774	cellular_component	vacuolar membrane
O	0005789	cellular_component	endoplasmic reticulum membrane
O	0007035	biological_process	vacuolar acidification
O	0008150	biological_process	biological_process
O	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
O	0033176	cellular_component	proton-transporting V-type ATPase complex
O	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
O	0048388	biological_process	endosomal lumen acidification
O	0061795	biological_process	Golgi lumen acidification
O	0090304	biological_process	nucleic acid metabolic process
O	1902600	biological_process	proton transmembrane transport

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1115
Details	Transmembrane: {"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	318
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	308
Details	Topological domain: {"description":"Vacuolar","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Site: {"description":"Essential for proton translocation","evidences":[{"source":"PubMed","id":"9030535","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	65
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	34
Details	Topological domain: {"description":"Vacuolar","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Topological domain: {"description":"Lumenal","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	8
Details	Site: {"description":"Essential for proton translocation","evidences":[{"source":"PubMed","id":"1825730","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	40
Details	Transmembrane: {"description":"Helical","evidences":[{"source":"PubMed","id":"30910982","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	22
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"30910982","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JUN-2005","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Peters C."]}},{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	28
Details	Coiled coil: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---