9MJ4
Yeast V-ATPase Vo proton channel bound to nanobody 2WVA149
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | TFS FALCON 4i (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.7 |
| Number of Particle | 369362 |
| Software | ISOLDE,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
