9FJF
Lysosomal transporting complex of beta-glucocerebrosidase (GCase) and lysosomal integral membrane protein 2 (LIMP-2) with bound Pro-macrobodies (Combined focus map)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005044 | molecular_function | scavenger receptor activity |
| A | 0005764 | cellular_component | lysosome |
| A | 0016020 | cellular_component | membrane |
| B | 0004336 | molecular_function | galactosylceramidase activity |
| B | 0004348 | molecular_function | glucosylceramidase activity |
| B | 0005102 | molecular_function | signaling receptor binding |
| B | 0005124 | molecular_function | scavenger receptor binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005615 | cellular_component | extracellular space |
| B | 0005764 | cellular_component | lysosome |
| B | 0005765 | cellular_component | lysosomal membrane |
| B | 0005783 | cellular_component | endoplasmic reticulum |
| B | 0005794 | cellular_component | Golgi apparatus |
| B | 0005802 | cellular_component | trans-Golgi network |
| B | 0006629 | biological_process | lipid metabolic process |
| B | 0006665 | biological_process | sphingolipid metabolic process |
| B | 0006680 | biological_process | glucosylceramide catabolic process |
| B | 0006914 | biological_process | autophagy |
| B | 0007005 | biological_process | mitochondrion organization |
| B | 0007040 | biological_process | lysosome organization |
| B | 0008202 | biological_process | steroid metabolic process |
| B | 0008203 | biological_process | cholesterol metabolic process |
| B | 0008422 | molecular_function | beta-glucosidase activity |
| B | 0009247 | biological_process | glycolipid biosynthetic process |
| B | 0009267 | biological_process | cellular response to starvation |
| B | 0009268 | biological_process | response to pH |
| B | 0010605 | biological_process | negative regulation of macromolecule metabolic process |
| B | 0016241 | biological_process | regulation of macroautophagy |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016757 | molecular_function | glycosyltransferase activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| B | 0023021 | biological_process | termination of signal transduction |
| B | 0030163 | biological_process | protein catabolic process |
| B | 0031175 | biological_process | neuron projection development |
| B | 0032006 | biological_process | regulation of TOR signaling |
| B | 0032715 | biological_process | negative regulation of interleukin-6 production |
| B | 0033574 | biological_process | response to testosterone |
| B | 0042176 | biological_process | regulation of protein catabolic process |
| B | 0042391 | biological_process | regulation of membrane potential |
| B | 0043202 | cellular_component | lysosomal lumen |
| B | 0043409 | biological_process | negative regulation of MAPK cascade |
| B | 0043627 | biological_process | response to estrogen |
| B | 0046512 | biological_process | sphingosine biosynthetic process |
| B | 0046513 | biological_process | ceramide biosynthetic process |
| B | 0046527 | molecular_function | glucosyltransferase activity |
| B | 0050295 | molecular_function | steryl-beta-glucosidase activity |
| B | 0050728 | biological_process | negative regulation of inflammatory response |
| B | 0061436 | biological_process | establishment of skin barrier |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 0071356 | biological_process | cellular response to tumor necrosis factor |
| B | 0071548 | biological_process | response to dexamethasone |
| B | 0097066 | biological_process | response to thyroid hormone |
| B | 0098773 | biological_process | skin epidermis development |
| B | 1901805 | biological_process | beta-glucoside catabolic process |
| B | 1904457 | biological_process | positive regulation of neuronal action potential |
| B | 1905146 | biological_process | lysosomal protein catabolic process |
| B | 1905165 | biological_process | regulation of lysosomal protein catabolic process |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 36 |
| Details | Region: {"description":"Important for interaction with GBA1"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 5 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24162852","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"24162852","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"12754519","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24162852","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"12754519","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24162852","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"15817452","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"15817452","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"12792654","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17139081","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"12754519","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17139081","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"12754519","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17139081","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"12754519","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 764 |
| Chain | Residue | Details |
| B | GLU235 | activator, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
| B | GLU340 | covalently attached, nucleofuge, nucleophile |
| B | CYS342 | electrostatic stabiliser |
| B | ASN370 |
