9FJF

Lysosomal transporting complex of beta-glucocerebrosidase (GCase) and lysosomal integral membrane protein 2 (LIMP-2) with bound Pro-macrobodies (Combined focus map)

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	TFS GLACIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Energy Filter	TFS Selectris
Detector	FEI FALCON IV (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3.7
Software	cryoSPARC,PHENIX,Coot,UCSF ChimeraX,EPU,MODELLER
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION