Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

9DTS

Crystal structure of the human eIF4A1/AMPPNP/amidino-rocaglate/polypurine RNA complex

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000339	molecular_function	RNA cap binding
A	0002183	biological_process	cytoplasmic translational initiation
A	0003723	molecular_function	RNA binding
A	0003724	molecular_function	RNA helicase activity
A	0003725	molecular_function	double-stranded RNA binding
A	0003729	molecular_function	mRNA binding
A	0003743	molecular_function	translation initiation factor activity
A	0004386	molecular_function	helicase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006412	biological_process	translation
A	0006413	biological_process	translational initiation
A	0008135	molecular_function	translation factor activity, RNA binding
A	0010494	cellular_component	cytoplasmic stress granule
A	0016020	cellular_component	membrane
A	0016281	cellular_component	eukaryotic translation initiation factor 4F complex
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0045944	biological_process	positive regulation of transcription by RNA polymerase II
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0070062	cellular_component	extracellular exosome
A	0097165	cellular_component	nuclear stress granule
B	0000166	molecular_function	nucleotide binding
B	0000339	molecular_function	RNA cap binding
B	0002183	biological_process	cytoplasmic translational initiation
B	0003723	molecular_function	RNA binding
B	0003724	molecular_function	RNA helicase activity
B	0003725	molecular_function	double-stranded RNA binding
B	0003729	molecular_function	mRNA binding
B	0003743	molecular_function	translation initiation factor activity
B	0004386	molecular_function	helicase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006412	biological_process	translation
B	0006413	biological_process	translational initiation
B	0008135	molecular_function	translation factor activity, RNA binding
B	0010494	cellular_component	cytoplasmic stress granule
B	0016020	cellular_component	membrane
B	0016281	cellular_component	eukaryotic translation initiation factor 4F complex
B	0016787	molecular_function	hydrolase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0045944	biological_process	positive regulation of transcription by RNA polymerase II
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0070062	cellular_component	extracellular exosome
B	0097165	cellular_component	nuclear stress granule
C	0000166	molecular_function	nucleotide binding
C	0000339	molecular_function	RNA cap binding
C	0002183	biological_process	cytoplasmic translational initiation
C	0003723	molecular_function	RNA binding
C	0003724	molecular_function	RNA helicase activity
C	0003725	molecular_function	double-stranded RNA binding
C	0003729	molecular_function	mRNA binding
C	0003743	molecular_function	translation initiation factor activity
C	0004386	molecular_function	helicase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006412	biological_process	translation
C	0006413	biological_process	translational initiation
C	0008135	molecular_function	translation factor activity, RNA binding
C	0010494	cellular_component	cytoplasmic stress granule
C	0016020	cellular_component	membrane
C	0016281	cellular_component	eukaryotic translation initiation factor 4F complex
C	0016787	molecular_function	hydrolase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0045944	biological_process	positive regulation of transcription by RNA polymerase II
C	0048471	cellular_component	perinuclear region of cytoplasm
C	0070062	cellular_component	extracellular exosome
C	0097165	cellular_component	nuclear stress granule
D	0000166	molecular_function	nucleotide binding
D	0000339	molecular_function	RNA cap binding
D	0002183	biological_process	cytoplasmic translational initiation
D	0003723	molecular_function	RNA binding
D	0003724	molecular_function	RNA helicase activity
D	0003725	molecular_function	double-stranded RNA binding
D	0003729	molecular_function	mRNA binding
D	0003743	molecular_function	translation initiation factor activity
D	0004386	molecular_function	helicase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006412	biological_process	translation
D	0006413	biological_process	translational initiation
D	0008135	molecular_function	translation factor activity, RNA binding
D	0010494	cellular_component	cytoplasmic stress granule
D	0016020	cellular_component	membrane
D	0016281	cellular_component	eukaryotic translation initiation factor 4F complex
D	0016787	molecular_function	hydrolase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0045944	biological_process	positive regulation of transcription by RNA polymerase II
D	0048471	cellular_component	perinuclear region of cytoplasm
D	0070062	cellular_component	extracellular exosome
D	0097165	cellular_component	nuclear stress granule

Functional Information from PROSITE/UniProt

site_id	PS00039
Number of Residues	9
Details	DEAD_ATP_HELICASE DEAD-box subfamily ATP-dependent helicases signature. VLDEADEmL

Chain	Residue	Details
A	VAL180-LEU188

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255\|PROSITE-ProRule:PRU00541

Chain	Residue	Details
A	ALA76
B	ALA76
C	ALA76
D	ALA76

site_id	SWS_FT_FI2
Number of Residues	8
Details	MOD_RES: N6-acetyllysine => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
A	LYS118
A	LYS174
B	LYS118
B	LYS174
C	LYS118
C	LYS174
D	LYS118
D	LYS174

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
A	THR158
B	THR158
C	THR158
D	THR158

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: N6-acetyllysine => ECO:0000250\|UniProtKB:P60843

Chain	Residue	Details
A	LYS193
B	LYS193
C	LYS193
D	LYS193

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0000250\|UniProtKB:P60843

Chain	Residue	Details
A	LYS238
B	LYS238
C	LYS238
D	LYS238

site_id	SWS_FT_FI6
Number of Residues	24
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744\|PubMed:28112733

Chain	Residue	Details
A	LYS146
B	LYS309
B	LYS369
B	LYS381
C	LYS146
C	LYS225
C	LYS309
C	LYS369
C	LYS381
D	LYS146
D	LYS225
D	LYS309
D	LYS369
D	LYS381
A	LYS225
A	LYS309
A	LYS369
A	LYS381
B	LYS146
B	LYS225

site_id	SWS_FT_FI7
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744\|PubMed:28112733

Chain	Residue	Details
A	LYS238
B	LYS238
C	LYS238
D	LYS238

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)