9DTS

Crystal structure of the human eIF4A1/AMPPNP/amidino-rocaglate/polypurine RNA complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920105
Spacegroup name	P 1
Unit cell lengths	66.110, 87.314, 93.159
Unit cell angles	95.22, 105.29, 108.35

Refinement procedure

Resolution	40.680 - 1.690
R-factor	0.2033
Rwork	0.203
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.268
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.362	1.750
High resolution limit [Å]	1.689	1.690
Rmerge	0.090	1.180
Rmeas	0.110	1.450
Rpim	0.061	0.840
Number of reflections	201775	19049
<I/σ(I)>	5.61
Completeness [%]	96.0	91.16
Redundancy	3	2.7
CC(1/2)	0.993	0.368

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	290	0.2 M ammonium acetate, 8% PEG20000, 10% Hampton Silver Bullet Reagent B5 (0.33% w/v 2,7-naphthalenedisulfonic acid disodium salt, 0.33% w/v azelaic acid, 0.33% w/v trans-cinnamic acid, 0.02 M HEPES sodium pH 6.8)