8WRZ

Cry-EM structure of cannabinoid receptor-beta-arrestin-1 complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000139	cellular_component	Golgi membrane
A	0000785	cellular_component	chromatin
A	0001664	molecular_function	G protein-coupled receptor binding
A	0001678	biological_process	intracellular glucose homeostasis
A	0001934	biological_process	positive regulation of protein phosphorylation
A	0002029	biological_process	desensitization of G protein-coupled receptor signaling pathway
A	0002031	biological_process	G protein-coupled receptor internalization
A	0002092	biological_process	positive regulation of receptor internalization
A	0003713	molecular_function	transcription coactivator activity
A	0004402	molecular_function	histone acetyltransferase activity
A	0004857	molecular_function	enzyme inhibitor activity
A	0004869	molecular_function	cysteine-type endopeptidase inhibitor activity
A	0005096	molecular_function	GTPase activator activity
A	0005159	molecular_function	insulin-like growth factor receptor binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005765	cellular_component	lysosomal membrane
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0005905	cellular_component	clathrin-coated pit
A	0005929	cellular_component	cilium
A	0006357	biological_process	regulation of transcription by RNA polymerase II
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0007165	biological_process	signal transduction
A	0007166	biological_process	cell surface receptor signaling pathway
A	0007600	biological_process	sensory perception
A	0016567	biological_process	protein ubiquitination
A	0019899	molecular_function	enzyme binding
A	0030659	cellular_component	cytoplasmic vesicle membrane
A	0030666	cellular_component	endocytic vesicle membrane
A	0030674	molecular_function	protein-macromolecule adaptor activity
A	0031143	cellular_component	pseudopodium
A	0031397	biological_process	negative regulation of protein ubiquitination
A	0031410	cellular_component	cytoplasmic vesicle
A	0031434	molecular_function	mitogen-activated protein kinase kinase binding
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0031701	molecular_function	angiotensin receptor binding
A	0032715	biological_process	negative regulation of interleukin-6 production
A	0032717	biological_process	negative regulation of interleukin-8 production
A	0035025	biological_process	positive regulation of Rho protein signal transduction
A	0035721	biological_process	intraciliary retrograde transport
A	0035774	biological_process	positive regulation of insulin secretion involved in cellular response to glucose stimulus
A	0042981	biological_process	regulation of apoptotic process
A	0043124	biological_process	negative regulation of canonical NF-kappaB signal transduction
A	0043149	biological_process	stress fiber assembly
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0045746	biological_process	negative regulation of Notch signaling pathway
A	0045880	biological_process	positive regulation of smoothened signaling pathway
A	0045944	biological_process	positive regulation of transcription by RNA polymerase II
A	0060090	molecular_function	molecular adaptor activity
A	0070374	biological_process	positive regulation of ERK1 and ERK2 cascade
A	0097499	biological_process	protein localization to non-motile cilium
A	1902533	biological_process	positive regulation of intracellular signal transduction
A	1903568	biological_process	negative regulation of protein localization to ciliary membrane
A	1990763	molecular_function	arrestin family protein binding
R	0004930	molecular_function	G protein-coupled receptor activity
R	0004949	molecular_function	cannabinoid receptor activity
R	0005506	molecular_function	iron ion binding
R	0007186	biological_process	G protein-coupled receptor signaling pathway
R	0009055	molecular_function	electron transfer activity
R	0016020	cellular_component	membrane
R	0020037	molecular_function	heme binding
R	0022900	biological_process	electron transport chain
R	0042597	cellular_component	periplasmic space

Functional Information from PROSITE/UniProt

site_id	PS00237
Number of Residues	17
Details	G_PROTEIN_RECEP_F1_1 G-protein coupled receptors family 1 signature. ASVgSLFLIAIDRYIsI

Chain	Residue	Details
R	ALA202-ILE218

---

site_id	PS00295
Number of Residues	19
Details	ARRESTINS Arrestins signature. FRYGrEDlDVLGLtFrKDL

Chain	Residue	Details
A	PHE61-LEU79

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	41
Details	Region: {"description":"Interaction with CHRM2","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"Q8BWG8","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	25
Details	Transmembrane: {"description":"Helical; Name=1","evidences":[{"source":"PubMed","id":"27768894","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27851727","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	30
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"27768894","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27851727","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	20
Details	Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"PubMed","id":"27768894","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27851727","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	39
Details	Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"27768894","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27851727","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	24
Details	Transmembrane: {"description":"Helical; Name=3","evidences":[{"source":"PubMed","id":"27768894","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27851727","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	22
Details	Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"PubMed","id":"27768894","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27851727","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	25
Details	Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"PubMed","id":"27768894","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27851727","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	20
Details	Transmembrane: {"description":"Helical; Name=6","evidences":[{"source":"PubMed","id":"27768894","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27851727","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	21
Details	Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"PubMed","id":"27768894","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27851727","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"37209686","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8GOC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8I10","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"37209686","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8GOC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8I10","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---