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8WEJ

Structure of human phagocyte NADPH oxidase in the activated state

Functional Information from GO Data
ChainGOidnamespacecontents
A0005515molecular_functionprotein binding
A0005737cellular_componentcytoplasm
A0005768cellular_componentendosome
A0005789cellular_componentendoplasmic reticulum membrane
A0005886cellular_componentplasma membrane
A0006801biological_processsuperoxide metabolic process
A0006954biological_processinflammatory response
A0009055molecular_functionelectron transfer activity
A0014895biological_processsmooth muscle hypertrophy
A0016020cellular_componentmembrane
A0016175molecular_functionsuperoxide-generating NAD(P)H oxidase activity
A0016491molecular_functionoxidoreductase activity
A0017004biological_processcytochrome complex assembly
A0017124molecular_functionSH3 domain binding
A0020037molecular_functionheme binding
A0030141cellular_componentsecretory granule
A0030670cellular_componentphagocytic vesicle membrane
A0032755biological_processpositive regulation of interleukin-6 production
A0032760biological_processpositive regulation of tumor necrosis factor production
A0034137biological_processpositive regulation of toll-like receptor 2 signaling pathway
A0035579cellular_componentspecific granule membrane
A0042554biological_processsuperoxide anion generation
A0043020cellular_componentNADPH oxidase complex
A0045087biological_processinnate immune response
A0045730biological_processrespiratory burst
A0046872molecular_functionmetal ion binding
A0046982molecular_functionprotein heterodimerization activity
A0050665biological_processhydrogen peroxide biosynthetic process
A0050766biological_processpositive regulation of phagocytosis
A0051279biological_processregulation of release of sequestered calcium ion into cytosol
A0051649biological_processestablishment of localization in cell
A0070254biological_processmucus secretion
A0070257biological_processpositive regulation of mucus secretion
A0070821cellular_componenttertiary granule membrane
A0072593biological_processreactive oxygen species metabolic process
A1900426biological_processpositive regulation of defense response to bacterium
A1903428biological_processpositive regulation of reactive oxygen species biosynthetic process
B0005515molecular_functionprotein binding
B0005635cellular_componentnuclear envelope
B0005737cellular_componentcytoplasm
B0005789cellular_componentendoplasmic reticulum membrane
B0005886cellular_componentplasma membrane
B0006801biological_processsuperoxide metabolic process
B0006952biological_processdefense response
B0006954biological_processinflammatory response
B0007584biological_processresponse to nutrient
B0009055molecular_functionelectron transfer activity
B0009410biological_processresponse to xenobiotic stimulus
B0016020cellular_componentmembrane
B0016175molecular_functionsuperoxide-generating NAD(P)H oxidase activity
B0016491molecular_functionoxidoreductase activity
B0020037molecular_functionheme binding
B0030425cellular_componentdendrite
B0030670cellular_componentphagocytic vesicle membrane
B0032760biological_processpositive regulation of tumor necrosis factor production
B0034220biological_processmonoatomic ion transmembrane transport
B0034702cellular_componentmonoatomic ion channel complex
B0035579cellular_componentspecific granule membrane
B0042554biological_processsuperoxide anion generation
B0043020cellular_componentNADPH oxidase complex
B0043025cellular_componentneuronal cell body
B0045087biological_processinnate immune response
B0045335cellular_componentphagocytic vesicle
B0045730biological_processrespiratory burst
B0045766biological_processpositive regulation of angiogenesis
B0046872molecular_functionmetal ion binding
B0046982molecular_functionprotein heterodimerization activity
B0050660molecular_functionflavin adenine dinucleotide binding
B0050664molecular_functionoxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
B0050665biological_processhydrogen peroxide biosynthetic process
B0070821cellular_componenttertiary granule membrane
B0071276biological_processcellular response to cadmium ion
B0071361biological_processcellular response to ethanol
B0071456biological_processcellular response to hypoxia
B0097038cellular_componentperinuclear endoplasmic reticulum
B0097411biological_processhypoxia-inducible factor-1alpha signaling pathway
B1904044biological_processresponse to aldosterone
B1904845biological_processcellular response to L-glutamine
B1990776biological_processresponse to angiotensin
C0005737cellular_componentcytoplasm
C0016176molecular_functionsuperoxide-generating NADPH oxidase activator activity
C0035091molecular_functionphosphatidylinositol binding
E0001764biological_processneuron migration
E0001934biological_processpositive regulation of protein phosphorylation
E0003376biological_processsphingosine-1-phosphate receptor signaling pathway
E0003924molecular_functionGTPase activity
E0003925molecular_functionG protein activity
E0005515molecular_functionprotein binding
E0005525molecular_functionGTP binding
E0005634cellular_componentnucleus
E0005737cellular_componentcytoplasm
E0005789cellular_componentendoplasmic reticulum membrane
E0005802cellular_componenttrans-Golgi network
E0005829cellular_componentcytosol
E0005856cellular_componentcytoskeleton
E0005884cellular_componentactin filament
E0005886cellular_componentplasma membrane
E0005925cellular_componentfocal adhesion
E0005938cellular_componentcell cortex
E0006954biological_processinflammatory response
E0007015biological_processactin filament organization
E0007155biological_processcell adhesion
E0007160biological_processcell-matrix adhesion
E0007163biological_processestablishment or maintenance of cell polarity
E0007264biological_processsmall GTPase-mediated signal transduction
E0008045biological_processmotor neuron axon guidance
E0008360biological_processregulation of cell shape
E0008361biological_processregulation of cell size
E0009611biological_processresponse to wounding
E0009653biological_processanatomical structure morphogenesis
E0010310biological_processregulation of hydrogen peroxide metabolic process
E0010591biological_processregulation of lamellipodium assembly
E0010592biological_processpositive regulation of lamellipodium assembly
E0010595biological_processpositive regulation of endothelial cell migration
E0010764biological_processnegative regulation of fibroblast migration
E0010811biological_processpositive regulation of cell-substrate adhesion
E0016020cellular_componentmembrane
E0016477biological_processcell migration
E0016601biological_processRac protein signal transduction
E0016787molecular_functionhydrolase activity
E0019899molecular_functionenzyme binding
E0019901molecular_functionprotein kinase binding
E0030027cellular_componentlamellipodium
E0030031biological_processcell projection assembly
E0030032biological_processlamellipodium assembly
E0030036biological_processactin cytoskeleton organization
E0030041biological_processactin filament polymerization
E0030334biological_processregulation of cell migration
E0030425cellular_componentdendrite
E0030667cellular_componentsecretory granule membrane
E0030865biological_processcortical cytoskeleton organization
E0031116biological_processpositive regulation of microtubule polymerization
E0031410cellular_componentcytoplasmic vesicle
E0031529biological_processruffle organization
E0031996molecular_functionthioesterase binding
E0032587cellular_componentruffle membrane
E0032707biological_processnegative regulation of interleukin-23 production
E0032956biological_processregulation of actin cytoskeleton organization
E0034446biological_processsubstrate adhesion-dependent cell spreading
E0035025biological_processpositive regulation of Rho protein signal transduction
E0035556biological_processintracellular signal transduction
E0036464cellular_componentcytoplasmic ribonucleoprotein granule
E0042470cellular_componentmelanosome
E0042995cellular_componentcell projection
E0043020cellular_componentNADPH oxidase complex
E0043197cellular_componentdendritic spine
E0043652biological_processengulfment of apoptotic cell
E0044877molecular_functionprotein-containing complex binding
E0045202cellular_componentsynapse
E0045428biological_processregulation of nitric oxide biosynthetic process
E0045730biological_processrespiratory burst
E0048012biological_processhepatocyte growth factor receptor signaling pathway
E0048261biological_processnegative regulation of receptor-mediated endocytosis
E0048870biological_processcell motility
E0051022molecular_functionRho GDP-dissociation inhibitor binding
E0051492biological_processregulation of stress fiber assembly
E0051496biological_processpositive regulation of stress fiber assembly
E0051668biological_processlocalization within membrane
E0051894biological_processpositive regulation of focal adhesion assembly
E0055038cellular_componentrecycling endosome membrane
E0060071biological_processWnt signaling pathway, planar cell polarity pathway
E0060263biological_processregulation of respiratory burst
E0060326biological_processcell chemotaxis
E0070062cellular_componentextracellular exosome
E0071526biological_processsemaphorin-plexin signaling pathway
E0090023biological_processpositive regulation of neutrophil chemotaxis
E0097178biological_processruffle assembly
E0098794cellular_componentpostsynapse
E0098978cellular_componentglutamatergic synapse
E0101003cellular_componentficolin-1-rich granule membrane
E1900026biological_processpositive regulation of substrate adhesion-dependent cell spreading
E1902622biological_processregulation of neutrophil migration
Functional Information from PROSITE/UniProt
site_idPS00290
Number of Residues7
DetailsIG_MHC Immunoglobulins and major histocompatibility complex proteins signature. YTCEATH
ChainResidueDetails
LTYR192-HIS198

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues3
DetailsBINDING: BINDING => ECO:0000269|PubMed:11090627, ECO:0007744|PDB:1E96, ECO:0007744|PDB:2WKP, ECO:0007744|PDB:2WKQ, ECO:0007744|PDB:2WKR
ChainResidueDetails
EALA13
EGLY30
EGLY60
BGLU283-PHE570

site_idSWS_FT_FI2
Number of Residues2
DetailsBINDING: BINDING => ECO:0000269|PubMed:11090627, ECO:0007744|PDB:1E96, ECO:0007744|PDB:2WKP, ECO:0007744|PDB:2WKQ, ECO:0007744|PDB:2WKR, ECO:0007744|PDB:5HZH
ChainResidueDetails
ELYS116
EALA159
BMET103-VAL123
BALA170-ILE190
BTYR201-ILE221
BPHE262-CYS282

site_idSWS_FT_FI3
Number of Residues1
DetailsMOD_RES: (Microbial infection) O-AMP-tyrosine; by Haemophilus IbpA; alternate => ECO:0000269|PubMed:19362538
ChainResidueDetails
ETYR32
BGLU124-LEU169
BHIS222-GLN261

site_idSWS_FT_FI4
Number of Residues1
DetailsMOD_RES: (Microbial infection) O-AMP-threonine; by Vibrio VopS => ECO:0000269|PubMed:19039103
ChainResidueDetails
ETHR35
BHIS115
BHIS209
BHIS222

site_idSWS_FT_FI5
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0000269|PubMed:10617634
ChainResidueDetails
ESER71

site_idSWS_FT_FI6
Number of Residues1
DetailsMOD_RES: Cysteine methyl ester => ECO:0000250|UniProtKB:P61585
ChainResidueDetails
ECYS189
BASN149
BASN240

site_idSWS_FT_FI7
Number of Residues2
DetailsLIPID: (Microbial infection) N6-palmitoyl lysine => ECO:0000269|PubMed:29074776
ChainResidueDetails
ELYS183
BLYS506
BLYS567
ELYS184
BLYS255
BLYS294
BLYS299
BLYS306
BLYS328
BLYS334
BLYS381

site_idSWS_FT_FI8
Number of Residues1
DetailsLIPID: S-geranylgeranyl cysteine => ECO:0000269|PubMed:1903399
ChainResidueDetails
ECYS189

site_idSWS_FT_FI9
Number of Residues1
DetailsCARBOHYD: (Microbial infection) O-linked (GlcNAc) tyrosine; by Photorhabdus PAU_02230; alternate => ECO:0000269|PubMed:24141704
ChainResidueDetails
ETYR32

site_idSWS_FT_FI10
Number of Residues1
DetailsCARBOHYD: (Microbial infection) O-linked (Glc) threonine; by C.difficile toxins TcdA and TcdB, and by P.sordellii toxin TcsL; alternate => ECO:0000269|PubMed:19744486, ECO:0000269|PubMed:24905543, ECO:0000269|PubMed:7775453, ECO:0000269|PubMed:7777059
ChainResidueDetails
ETHR35

site_idSWS_FT_FI11
Number of Residues1
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:18093184
ChainResidueDetails
ELYS147

site_idSWS_FT_FI12
Number of Residues2
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:23512198
ChainResidueDetails
ELYS166

222415

PDB entries from 2024-07-10

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