8WEJ

Structure of human phagocyte NADPH oxidase in the activated state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001666	biological_process	response to hypoxia
A	0001725	cellular_component	stress fiber
A	0001938	biological_process	positive regulation of endothelial cell proliferation
A	0003106	biological_process	negative regulation of glomerular filtration by angiotensin
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005768	cellular_component	endosome
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0005886	cellular_component	plasma membrane
A	0005925	cellular_component	focal adhesion
A	0006801	biological_process	superoxide metabolic process
A	0006954	biological_process	inflammatory response
A	0009055	molecular_function	electron transfer activity
A	0009410	biological_process	response to xenobiotic stimulus
A	0014823	biological_process	response to activity
A	0014895	biological_process	smooth muscle hypertrophy
A	0016020	cellular_component	membrane
A	0016175	molecular_function	superoxide-generating NAD(P)H oxidase activity
A	0016324	cellular_component	apical plasma membrane
A	0016491	molecular_function	oxidoreductase activity
A	0017004	biological_process	cytochrome complex assembly
A	0017124	molecular_function	SH3 domain binding
A	0020037	molecular_function	heme binding
A	0030141	cellular_component	secretory granule
A	0030307	biological_process	positive regulation of cell growth
A	0030425	cellular_component	dendrite
A	0030670	cellular_component	phagocytic vesicle membrane
A	0031667	biological_process	response to nutrient levels
A	0032755	biological_process	positive regulation of interleukin-6 production
A	0032760	biological_process	positive regulation of tumor necrosis factor production
A	0032930	biological_process	positive regulation of superoxide anion generation
A	0034137	biological_process	positive regulation of toll-like receptor 2 signaling pathway
A	0035579	cellular_component	specific granule membrane
A	0042554	biological_process	superoxide anion generation
A	0043020	cellular_component	NADPH oxidase complex
A	0043025	cellular_component	neuronal cell body
A	0045087	biological_process	innate immune response
A	0045730	biological_process	respiratory burst
A	0045777	biological_process	positive regulation of blood pressure
A	0046872	molecular_function	metal ion binding
A	0046982	molecular_function	protein heterodimerization activity
A	0048661	biological_process	positive regulation of smooth muscle cell proliferation
A	0050665	biological_process	hydrogen peroxide biosynthetic process
A	0050766	biological_process	positive regulation of phagocytosis
A	0070555	biological_process	response to interleukin-1
A	0070821	cellular_component	tertiary granule membrane
A	0071260	biological_process	cellular response to mechanical stimulus
A	0071333	biological_process	cellular response to glucose stimulus
A	0071356	biological_process	cellular response to tumor necrosis factor
A	0071480	biological_process	cellular response to gamma radiation
A	0097038	cellular_component	perinuclear endoplasmic reticulum
A	1900426	biological_process	positive regulation of defense response to bacterium
A	1903428	biological_process	positive regulation of reactive oxygen species biosynthetic process
A	1904044	biological_process	response to aldosterone
A	1904385	biological_process	cellular response to angiotensin
A	1904628	biological_process	cellular response to phorbol 13-acetate 12-myristate
A	1904845	biological_process	cellular response to L-glutamine
B	0005515	molecular_function	protein binding
B	0005635	cellular_component	nuclear envelope
B	0005737	cellular_component	cytoplasm
B	0005789	cellular_component	endoplasmic reticulum membrane
B	0005886	cellular_component	plasma membrane
B	0006801	biological_process	superoxide metabolic process
B	0006811	biological_process	monoatomic ion transport
B	0006952	biological_process	defense response
B	0006954	biological_process	inflammatory response
B	0007584	biological_process	response to nutrient
B	0009055	molecular_function	electron transfer activity
B	0009410	biological_process	response to xenobiotic stimulus
B	0016020	cellular_component	membrane
B	0016174	molecular_function	NAD(P)H oxidase H2O2-forming activity
B	0016175	molecular_function	superoxide-generating NAD(P)H oxidase activity
B	0016491	molecular_function	oxidoreductase activity
B	0020037	molecular_function	heme binding
B	0030171	molecular_function	voltage-gated proton channel activity
B	0030425	cellular_component	dendrite
B	0030670	cellular_component	phagocytic vesicle membrane
B	0032760	biological_process	positive regulation of tumor necrosis factor production
B	0034220	biological_process	monoatomic ion transmembrane transport
B	0034702	cellular_component	monoatomic ion channel complex
B	0035579	cellular_component	specific granule membrane
B	0042554	biological_process	superoxide anion generation
B	0043020	cellular_component	NADPH oxidase complex
B	0043025	cellular_component	neuronal cell body
B	0045087	biological_process	innate immune response
B	0045335	cellular_component	phagocytic vesicle
B	0045471	biological_process	response to ethanol
B	0045730	biological_process	respiratory burst
B	0045766	biological_process	positive regulation of angiogenesis
B	0046872	molecular_function	metal ion binding
B	0046982	molecular_function	protein heterodimerization activity
B	0050660	molecular_function	flavin adenine dinucleotide binding
B	0050664	molecular_function	oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
B	0070402	molecular_function	NADPH binding
B	0070821	cellular_component	tertiary granule membrane
B	0071276	biological_process	cellular response to cadmium ion
B	0071361	biological_process	cellular response to ethanol
B	0071456	biological_process	cellular response to hypoxia
B	0071949	molecular_function	FAD binding
B	0097038	cellular_component	perinuclear endoplasmic reticulum
B	0097411	biological_process	hypoxia-inducible factor-1alpha signaling pathway
B	0106292	molecular_function	superoxide-generating NADPH oxidase activity
B	1904044	biological_process	response to aldosterone
B	1904845	biological_process	cellular response to L-glutamine
B	1990776	biological_process	response to angiotensin
C	0005737	cellular_component	cytoplasm
C	0016176	molecular_function	superoxide-generating NADPH oxidase activator activity
C	0035091	molecular_function	phosphatidylinositol binding
E	0000166	molecular_function	nucleotide binding
E	0000242	cellular_component	pericentriolar material
E	0001764	biological_process	neuron migration
E	0001891	cellular_component	phagocytic cup
E	0001934	biological_process	positive regulation of protein phosphorylation
E	0003376	biological_process	sphingosine-1-phosphate receptor signaling pathway
E	0003924	molecular_function	GTPase activity
E	0003925	molecular_function	G protein activity
E	0005515	molecular_function	protein binding
E	0005525	molecular_function	GTP binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005730	cellular_component	nucleolus
E	0005737	cellular_component	cytoplasm
E	0005789	cellular_component	endoplasmic reticulum membrane
E	0005802	cellular_component	trans-Golgi network
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0005884	cellular_component	actin filament
E	0005886	cellular_component	plasma membrane
E	0005925	cellular_component	focal adhesion
E	0005938	cellular_component	cell cortex
E	0006911	biological_process	phagocytosis, engulfment
E	0006954	biological_process	inflammatory response
E	0007015	biological_process	actin filament organization
E	0007155	biological_process	cell adhesion
E	0007160	biological_process	cell-matrix adhesion
E	0007163	biological_process	establishment or maintenance of cell polarity
E	0007167	biological_process	enzyme-linked receptor protein signaling pathway
E	0007264	biological_process	small GTPase-mediated signal transduction
E	0008045	biological_process	motor neuron axon guidance
E	0008360	biological_process	regulation of cell shape
E	0009611	biological_process	response to wounding
E	0009653	biological_process	anatomical structure morphogenesis
E	0010310	biological_process	regulation of hydrogen peroxide metabolic process
E	0010591	biological_process	regulation of lamellipodium assembly
E	0010592	biological_process	positive regulation of lamellipodium assembly
E	0010595	biological_process	positive regulation of endothelial cell migration
E	0010762	biological_process	regulation of fibroblast migration
E	0010764	biological_process	negative regulation of fibroblast migration
E	0010810	biological_process	regulation of cell-substrate adhesion
E	0016020	cellular_component	membrane
E	0016477	biological_process	cell migration
E	0016601	biological_process	Rac protein signal transduction
E	0016787	molecular_function	hydrolase activity
E	0019899	molecular_function	enzyme binding
E	0019901	molecular_function	protein kinase binding
E	0030027	cellular_component	lamellipodium
E	0030031	biological_process	cell projection assembly
E	0030032	biological_process	lamellipodium assembly
E	0030036	biological_process	actin cytoskeleton organization
E	0030041	biological_process	actin filament polymerization
E	0030334	biological_process	regulation of cell migration
E	0030425	cellular_component	dendrite
E	0030667	cellular_component	secretory granule membrane
E	0030672	cellular_component	synaptic vesicle membrane
E	0030742	molecular_function	GTP-dependent protein binding
E	0030865	biological_process	cortical cytoskeleton organization
E	0030900	biological_process	forebrain development
E	0031410	cellular_component	cytoplasmic vesicle
E	0031529	biological_process	ruffle organization
E	0031901	cellular_component	early endosome membrane
E	0031996	molecular_function	thioesterase binding
E	0032496	biological_process	response to lipopolysaccharide
E	0032587	cellular_component	ruffle membrane
E	0032707	biological_process	negative regulation of interleukin-23 production
E	0032956	biological_process	regulation of actin cytoskeleton organization
E	0034446	biological_process	substrate adhesion-dependent cell spreading
E	0035025	biological_process	positive regulation of Rho protein signal transduction
E	0035556	biological_process	intracellular signal transduction
E	0035567	biological_process	non-canonical Wnt signaling pathway
E	0035774	biological_process	positive regulation of insulin secretion involved in cellular response to glucose stimulus
E	0036464	cellular_component	cytoplasmic ribonucleoprotein granule
E	0042470	cellular_component	melanosome
E	0042554	biological_process	superoxide anion generation
E	0042734	cellular_component	presynaptic membrane
E	0042995	cellular_component	cell projection
E	0043020	cellular_component	NADPH oxidase complex
E	0043652	biological_process	engulfment of apoptotic cell
E	0044877	molecular_function	protein-containing complex binding
E	0045202	cellular_component	synapse
E	0045211	cellular_component	postsynaptic membrane
E	0045428	biological_process	regulation of nitric oxide biosynthetic process
E	0045730	biological_process	respiratory burst
E	0046425	biological_process	regulation of receptor signaling pathway via JAK-STAT
E	0048012	biological_process	hepatocyte growth factor receptor signaling pathway
E	0048261	biological_process	negative regulation of receptor-mediated endocytosis
E	0048812	biological_process	neuron projection morphogenesis
E	0048870	biological_process	cell motility
E	0051022	molecular_function	Rho GDP-dissociation inhibitor binding
E	0051492	biological_process	regulation of stress fiber assembly
E	0051496	biological_process	positive regulation of stress fiber assembly
E	0051668	biological_process	localization within membrane
E	0051894	biological_process	positive regulation of focal adhesion assembly
E	0055038	cellular_component	recycling endosome membrane
E	0060071	biological_process	Wnt signaling pathway, planar cell polarity pathway
E	0060091	cellular_component	kinocilium
E	0060263	biological_process	regulation of respiratory burst
E	0060326	biological_process	cell chemotaxis
E	0061344	biological_process	regulation of cell adhesion involved in heart morphogenesis
E	0070062	cellular_component	extracellular exosome
E	0071526	biological_process	semaphorin-plexin signaling pathway
E	0090023	biological_process	positive regulation of neutrophil chemotaxis
E	0097178	biological_process	ruffle assembly
E	0098794	cellular_component	postsynapse
E	0098978	cellular_component	glutamatergic synapse
E	0101003	cellular_component	ficolin-1-rich granule membrane
E	0150052	biological_process	regulation of postsynapse assembly
E	1900026	biological_process	positive regulation of substrate adhesion-dependent cell spreading
E	1900029	biological_process	positive regulation of ruffle assembly
E	1900242	biological_process	regulation of synaptic vesicle endocytosis
E	1902622	biological_process	regulation of neutrophil migration
E	1903348	biological_process	positive regulation of bicellular tight junction assembly
E	1904948	biological_process	midbrain dopaminergic neuron differentiation
E	2000386	biological_process	positive regulation of ovarian follicle development

Functional Information from PROSITE/UniProt

site_id	PS00290
Number of Residues	7
Details	IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. YTCEATH

Chain	Residue	Details
L	TYR192-HIS198

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	226
Details	Transmembrane: {"description":"Helical","evidences":[{"source":"PubMed","id":"36241643","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36413210","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7U8G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8GZ3","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	5
Details	Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"36241643","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"36413210","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	20
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"36241643","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"36413210","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	10
Details	Intramembrane: {"evidences":[{"source":"PubMed","id":"36241643","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	321
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"36413210","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	85
Details	Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"36413210","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	232
Details	Domain: {"description":"Ferric oxidoreductase"}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	110
Details	Domain: {"description":"FAD-binding FR-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU00716","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	Binding site: {"description":"axial binding residue","evidences":[{"source":"PubMed","id":"36413210","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38355798","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8GZ3","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36413210","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8GZ3","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36413210","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38355798","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8GZ3","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"38355798","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	2
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36413210","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8GZ3","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	1
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	11
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"Q61093","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	33
Details	Repeat: {"description":"TPR 1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	33
Details	Repeat: {"description":"TPR 2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	33
Details	Repeat: {"description":"TPR 3"}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	8
Details	Motif: {"description":"Effector region","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"38332367","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8Q0N","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI21
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11090627","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38332367","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38355798","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1E96","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Q0N","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8WEJ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI22
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11090627","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38332367","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1E96","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Q0N","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI23
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11090627","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1E96","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI24
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"38355798","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8WEJ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI25
Number of Residues	1
Details	Modified residue: {"description":"(Microbial infection) O-AMP-tyrosine; by Haemophilus IbpA; alternate","evidences":[{"source":"PubMed","id":"19362538","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI26
Number of Residues	1
Details	Modified residue: {"description":"(Microbial infection) O-AMP-threonine; by Vibrio VopS","evidences":[{"source":"PubMed","id":"19039103","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI27
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI28
Number of Residues	1
Details	Glycosylation: {"description":"(Microbial infection) O-linked (GlcNAc) tyrosine; by Photorhabdus PAU_02230; alternate","evidences":[{"source":"PubMed","id":"24141704","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI29
Number of Residues	1
Details	Glycosylation: {"description":"(Microbial infection) O-linked (Glc) threonine; by C.difficile toxins TcdA and TcdB, and by P.sordellii toxin TcsL; alternate","evidences":[{"source":"PubMed","id":"19744486","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24905543","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7775453","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7777059","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI30
Number of Residues	1
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"18093184","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38332367","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI31
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"23512198","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---