8W50
Crystal structure of DNA binding and cleavage core of human topoisomerase 2-alpha in a DNA binding-competent conformation
Functional Information from PROSITE/UniProt
| site_id | PS00177 |
| Number of Residues | 9 |
| Details | TOPOISOMERASE_II DNA topoisomerase II signature. LTEGDSAKT |
| Chain | Residue | Details |
| A | LEU459-THR467 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: O-(5'-phospho-DNA)-tyrosine intermediate => ECO:0000255|PROSITE-ProRule:PRU01384 |
| Chain | Residue | Details |
| A | TYR805 | |
| B | TYR805 | |
| C | TYR805 | |
| D | TYR805 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00995 |
| Chain | Residue | Details |
| A | GLU461 | |
| B | GLU461 | |
| C | GLU461 | |
| D | GLU461 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:22841979 |
| Chain | Residue | Details |
| A | ASP541 | |
| A | ASP543 | |
| B | ASP541 | |
| B | ASP543 | |
| C | ASP541 | |
| C | ASP543 | |
| D | ASP541 | |
| D | ASP543 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | SITE: Interaction with DNA => ECO:0000255|PROSITE-ProRule:PRU00995 |
| Chain | Residue | Details |
| A | LYS489 | |
| B | LYS489 | |
| C | LYS489 | |
| D | LYS489 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 28 |
| Details | SITE: Interaction with DNA => ECO:0000269|PubMed:22841979 |
| Chain | Residue | Details |
| A | ASN492 | |
| B | LYS662 | |
| B | LYS723 | |
| B | TYR757 | |
| B | SER763 | |
| B | TRP931 | |
| C | ASN492 | |
| C | ARG661 | |
| C | LYS662 | |
| C | LYS723 | |
| C | TYR757 | |
| A | ARG661 | |
| C | SER763 | |
| C | TRP931 | |
| D | ASN492 | |
| D | ARG661 | |
| D | LYS662 | |
| D | LYS723 | |
| D | TYR757 | |
| D | SER763 | |
| D | TRP931 | |
| A | LYS662 | |
| A | LYS723 | |
| A | TYR757 | |
| A | SER763 | |
| A | TRP931 | |
| B | ASN492 | |
| B | ARG661 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | SITE: Transition state stabilizer => ECO:0000250|UniProtKB:P06786 |
| Chain | Residue | Details |
| A | ARG804 | |
| B | ARG804 | |
| C | ARG804 | |
| D | ARG804 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | SITE: Important for DNA bending; intercalates between base pairs of target DNA => ECO:0000250|UniProtKB:P06786 |
| Chain | Residue | Details |
| A | ILE856 | |
| B | ILE856 | |
| C | ILE856 | |
| D | ILE856 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine; by CK1 => ECO:0000269|PubMed:19043076, ECO:0000269|Ref.11, ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | SER1106 | |
| B | SER1106 | |
| C | SER1106 | |
| D | SER1106 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 52 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733 |
| Chain | Residue | Details |
| A | LYS440 | |
| A | LYS625 | |
| A | LYS632 | |
| A | LYS655 | |
| A | LYS1114 | |
| B | LYS440 | |
| B | LYS466 | |
| B | LYS480 | |
| B | LYS529 | |
| B | LYS584 | |
| B | LYS599 | |
| B | LYS614 | |
| B | LYS622 | |
| B | LYS625 | |
| B | LYS632 | |
| B | LYS655 | |
| B | LYS1114 | |
| C | LYS440 | |
| C | LYS466 | |
| A | LYS466 | |
| C | LYS480 | |
| C | LYS529 | |
| C | LYS584 | |
| C | LYS599 | |
| C | LYS614 | |
| C | LYS622 | |
| C | LYS625 | |
| C | LYS632 | |
| C | LYS655 | |
| C | LYS1114 | |
| A | LYS480 | |
| D | LYS440 | |
| D | LYS466 | |
| D | LYS480 | |
| D | LYS529 | |
| D | LYS584 | |
| D | LYS599 | |
| D | LYS614 | |
| D | LYS622 | |
| D | LYS625 | |
| A | LYS529 | |
| D | LYS632 | |
| D | LYS655 | |
| D | LYS1114 | |
| A | LYS584 | |
| A | LYS599 | |
| A | LYS614 | |
| A | LYS622 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 16 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25772364, ECO:0007744|PubMed:28112733 |
| Chain | Residue | Details |
| A | LYS639 | |
| C | LYS662 | |
| C | LYS676 | |
| C | LYS1075 | |
| D | LYS639 | |
| D | LYS662 | |
| D | LYS676 | |
| D | LYS1075 | |
| A | LYS662 | |
| A | LYS676 | |
| A | LYS1075 | |
| B | LYS639 | |
| B | LYS662 | |
| B | LYS676 | |
| B | LYS1075 | |
| C | LYS639 |
