8T50

Open human HCN1 F186C S264C bound to cAMP, reconstituted in LMNG + SPL

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003254	biological_process	regulation of membrane depolarization
A	0005222	molecular_function	intracellularly cAMP-activated cation channel activity
A	0005248	molecular_function	voltage-gated sodium channel activity
A	0005249	molecular_function	voltage-gated potassium channel activity
A	0005267	molecular_function	potassium channel activity
A	0005272	molecular_function	sodium channel activity
A	0005515	molecular_function	protein binding
A	0005886	cellular_component	plasma membrane
A	0006814	biological_process	sodium ion transport
A	0019228	biological_process	neuronal action potential
A	0022843	molecular_function	voltage-gated monoatomic cation channel activity
A	0030424	cellular_component	axon
A	0030425	cellular_component	dendrite
A	0030552	molecular_function	cAMP binding
A	0034702	cellular_component	monoatomic ion channel complex
A	0035725	biological_process	sodium ion transmembrane transport
A	0042391	biological_process	regulation of membrane potential
A	0042802	molecular_function	identical protein binding
A	0045176	biological_process	apical protein localization
A	0045211	cellular_component	postsynaptic membrane
A	0046549	biological_process	retinal cone cell development
A	0048787	cellular_component	presynaptic active zone membrane
A	0051289	biological_process	protein homotetramerization
A	0051867	biological_process	general adaptation syndrome, behavioral process
A	0060078	biological_process	regulation of postsynaptic membrane potential
A	0071320	biological_process	cellular response to cAMP
A	0071805	biological_process	potassium ion transmembrane transport
A	0098855	cellular_component	HCN channel complex
A	0098978	cellular_component	glutamatergic synapse
A	0140232	molecular_function	intracellular cAMP-activated cation channel activity involved in regulation of presynaptic membrane potential
B	0003254	biological_process	regulation of membrane depolarization
B	0005222	molecular_function	intracellularly cAMP-activated cation channel activity
B	0005248	molecular_function	voltage-gated sodium channel activity
B	0005249	molecular_function	voltage-gated potassium channel activity
B	0005267	molecular_function	potassium channel activity
B	0005272	molecular_function	sodium channel activity
B	0005515	molecular_function	protein binding
B	0005886	cellular_component	plasma membrane
B	0006814	biological_process	sodium ion transport
B	0019228	biological_process	neuronal action potential
B	0022843	molecular_function	voltage-gated monoatomic cation channel activity
B	0030424	cellular_component	axon
B	0030425	cellular_component	dendrite
B	0030552	molecular_function	cAMP binding
B	0034702	cellular_component	monoatomic ion channel complex
B	0035725	biological_process	sodium ion transmembrane transport
B	0042391	biological_process	regulation of membrane potential
B	0042802	molecular_function	identical protein binding
B	0045176	biological_process	apical protein localization
B	0045211	cellular_component	postsynaptic membrane
B	0046549	biological_process	retinal cone cell development
B	0048787	cellular_component	presynaptic active zone membrane
B	0051289	biological_process	protein homotetramerization
B	0051867	biological_process	general adaptation syndrome, behavioral process
B	0060078	biological_process	regulation of postsynaptic membrane potential
B	0071320	biological_process	cellular response to cAMP
B	0071805	biological_process	potassium ion transmembrane transport
B	0098855	cellular_component	HCN channel complex
B	0098978	cellular_component	glutamatergic synapse
B	0140232	molecular_function	intracellular cAMP-activated cation channel activity involved in regulation of presynaptic membrane potential
C	0003254	biological_process	regulation of membrane depolarization
C	0005222	molecular_function	intracellularly cAMP-activated cation channel activity
C	0005248	molecular_function	voltage-gated sodium channel activity
C	0005249	molecular_function	voltage-gated potassium channel activity
C	0005267	molecular_function	potassium channel activity
C	0005272	molecular_function	sodium channel activity
C	0005515	molecular_function	protein binding
C	0005886	cellular_component	plasma membrane
C	0006814	biological_process	sodium ion transport
C	0019228	biological_process	neuronal action potential
C	0022843	molecular_function	voltage-gated monoatomic cation channel activity
C	0030424	cellular_component	axon
C	0030425	cellular_component	dendrite
C	0030552	molecular_function	cAMP binding
C	0034702	cellular_component	monoatomic ion channel complex
C	0035725	biological_process	sodium ion transmembrane transport
C	0042391	biological_process	regulation of membrane potential
C	0042802	molecular_function	identical protein binding
C	0045176	biological_process	apical protein localization
C	0045211	cellular_component	postsynaptic membrane
C	0046549	biological_process	retinal cone cell development
C	0048787	cellular_component	presynaptic active zone membrane
C	0051289	biological_process	protein homotetramerization
C	0051867	biological_process	general adaptation syndrome, behavioral process
C	0060078	biological_process	regulation of postsynaptic membrane potential
C	0071320	biological_process	cellular response to cAMP
C	0071805	biological_process	potassium ion transmembrane transport
C	0098855	cellular_component	HCN channel complex
C	0098978	cellular_component	glutamatergic synapse
C	0140232	molecular_function	intracellular cAMP-activated cation channel activity involved in regulation of presynaptic membrane potential
D	0003254	biological_process	regulation of membrane depolarization
D	0005222	molecular_function	intracellularly cAMP-activated cation channel activity
D	0005248	molecular_function	voltage-gated sodium channel activity
D	0005249	molecular_function	voltage-gated potassium channel activity
D	0005267	molecular_function	potassium channel activity
D	0005272	molecular_function	sodium channel activity
D	0005515	molecular_function	protein binding
D	0005886	cellular_component	plasma membrane
D	0006814	biological_process	sodium ion transport
D	0019228	biological_process	neuronal action potential
D	0022843	molecular_function	voltage-gated monoatomic cation channel activity
D	0030424	cellular_component	axon
D	0030425	cellular_component	dendrite
D	0030552	molecular_function	cAMP binding
D	0034702	cellular_component	monoatomic ion channel complex
D	0035725	biological_process	sodium ion transmembrane transport
D	0042391	biological_process	regulation of membrane potential
D	0042802	molecular_function	identical protein binding
D	0045176	biological_process	apical protein localization
D	0045211	cellular_component	postsynaptic membrane
D	0046549	biological_process	retinal cone cell development
D	0048787	cellular_component	presynaptic active zone membrane
D	0051289	biological_process	protein homotetramerization
D	0051867	biological_process	general adaptation syndrome, behavioral process
D	0060078	biological_process	regulation of postsynaptic membrane potential
D	0071320	biological_process	cellular response to cAMP
D	0071805	biological_process	potassium ion transmembrane transport
D	0098855	cellular_component	HCN channel complex
D	0098978	cellular_component	glutamatergic synapse
D	0140232	molecular_function	intracellular cAMP-activated cation channel activity involved in regulation of presynaptic membrane potential

Functional Information from PROSITE/UniProt

site_id	PS00888
Number of Residues	17
Details	CNMP_BINDING_1 Cyclic nucleotide-binding domain signature 1. IIrEGAvGKkMYFIqhG

Chain	Residue	Details
C	ILE502-GLY518

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2684
Details	TOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:28086084

Chain	Residue	Details
C	MET1-ARG142
A	ARG195-MET215
A	GLU282-VAL295
A	ALA393-LEU890
D	MET1-ARG142
D	ARG195-MET215
D	GLU282-VAL295
D	ALA393-LEU890
C	ARG195-MET215
C	GLU282-VAL295
C	ALA393-LEU890
B	MET1-ARG142
B	ARG195-MET215
B	GLU282-VAL295
B	ALA393-LEU890
A	MET1-ARG142

---

site_id	SWS_FT_FI2
Number of Residues	84
Details	TRANSMEM: Helical; Name=Segment S1 => ECO:0000269|PubMed:28086084, ECO:0000269|PubMed:31787376, ECO:0007744|PDB:5U6O, ECO:0007744|PDB:5U6P, ECO:0007744|PDB:6UQF, ECO:0007744|PDB:6UQG

Chain	Residue	Details
C	PHE143-ILE164
B	PHE143-ILE164
A	PHE143-ILE164
D	PHE143-ILE164

---

site_id	SWS_FT_FI3
Number of Residues	240
Details	TOPO_DOM: Extracellular => ECO:0000269|PubMed:28086084

Chain	Residue	Details
C	THR165-THR173
A	LEU237-THR260
A	VAL319-GLN344
A	VAL367-ASP371
D	THR165-THR173
D	LEU237-THR260
D	VAL319-GLN344
D	VAL367-ASP371
C	LEU237-THR260
C	VAL319-GLN344
C	VAL367-ASP371
B	THR165-THR173
B	LEU237-THR260
B	VAL319-GLN344
B	VAL367-ASP371
A	THR165-THR173

---

site_id	SWS_FT_FI4
Number of Residues	80
Details	TRANSMEM: Helical; Name=Segment S2 => ECO:0000269|PubMed:28086084, ECO:0000269|PubMed:31787376, ECO:0007744|PDB:5U6O, ECO:0007744|PDB:5U6P, ECO:0007744|PDB:6UQF, ECO:0007744|PDB:6UQG

Chain	Residue	Details
C	PRO174-PHE194
B	PRO174-PHE194
A	PRO174-PHE194
D	PRO174-PHE194

---

site_id	SWS_FT_FI5
Number of Residues	80
Details	TRANSMEM: Helical; Name=Segment S3 => ECO:0000269|PubMed:28086084, ECO:0000269|PubMed:31787376, ECO:0007744|PDB:5U6O, ECO:0007744|PDB:5U6P, ECO:0007744|PDB:6UQF, ECO:0007744|PDB:6UQG

Chain	Residue	Details
C	ASN216-PHE236
B	ASN216-PHE236
A	ASN216-PHE236
D	ASN216-PHE236

---

site_id	SWS_FT_FI6
Number of Residues	80
Details	TRANSMEM: Helical; Voltage-sensor; Name=Segment S4 => ECO:0000269|PubMed:28086084, ECO:0000269|PubMed:31787376, ECO:0007744|PDB:5U6O, ECO:0007744|PDB:5U6P, ECO:0007744|PDB:6UQF, ECO:0007744|PDB:6UQG

Chain	Residue	Details
C	LYS261-TRP281
B	LYS261-TRP281
A	LYS261-TRP281
D	LYS261-TRP281

---

site_id	SWS_FT_FI7
Number of Residues	88
Details	TRANSMEM: Helical; Name=Segment S5 => ECO:0007744|PDB:5U6O, ECO:0007744|PDB:5U6P, ECO:0007744|PDB:6UQF, ECO:0007744|PDB:6UQG

Chain	Residue	Details
C	VAL296-LEU318
B	VAL296-LEU318
A	VAL296-LEU318
D	VAL296-LEU318

---

site_id	SWS_FT_FI8
Number of Residues	84
Details	INTRAMEM: Pore-forming; Name=Segment H5 => ECO:0000269|PubMed:28086084, ECO:0000269|PubMed:31787376, ECO:0007744|PDB:5U6O, ECO:0007744|PDB:5U6P, ECO:0007744|PDB:6UQF, ECO:0007744|PDB:6UQG

Chain	Residue	Details
C	TYR345-PRO366
B	TYR345-PRO366
A	TYR345-PRO366
D	TYR345-PRO366

---

site_id	SWS_FT_FI9
Number of Residues	80
Details	TRANSMEM: Helical; Name=Segment S6 => ECO:0000269|PubMed:28086084, ECO:0000269|PubMed:31787376, ECO:0007744|PDB:5U6O, ECO:0007744|PDB:5U6P, ECO:0007744|PDB:6UQF, ECO:0007744|PDB:6UQG

Chain	Residue	Details
C	LEU372-HIS392
B	LEU372-HIS392
A	LEU372-HIS392
D	LEU372-HIS392

---

site_id	SWS_FT_FI10
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269|PubMed:31787376, ECO:0000305|PubMed:28086084, ECO:0007744|PDB:5U6P, ECO:0007744|PDB:6UQF

Chain	Residue	Details
C	GLY539
A	CYS542
A	ARG549
A	THR550
D	GLY539
D	CYS542
D	ARG549
D	THR550
C	CYS542
C	ARG549
C	THR550
B	GLY539
B	CYS542
B	ARG549
B	THR550
A	GLY539

---

site_id	SWS_FT_FI11
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269|PubMed:31787376, ECO:0007744|PDB:6UQF

Chain	Residue	Details
C	GLU540
C	ARG593
B	GLU540
B	ARG593
A	GLU540
A	ARG593
D	GLU540
D	ARG593

---

site_id	SWS_FT_FI12
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250|UniProtKB:O88704

Chain	Residue	Details
C	ARG590
B	ARG590
A	ARG590
D	ARG590

---

site_id	SWS_FT_FI13
Number of Residues	4
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
C	ASN338
B	ASN338
A	ASN338
D	ASN338

---