Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8RW1

Structure of a yeast 48S-AUC preinitiation complex in closed conformation

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000028	biological_process	ribosomal small subunit assembly
A	0003735	molecular_function	structural constituent of ribosome
A	0005737	cellular_component	cytoplasm
A	0005840	cellular_component	ribosome
A	0006412	biological_process	translation
A	0022627	cellular_component	cytosolic small ribosomal subunit
A	1990904	cellular_component	ribonucleoprotein complex
B	0003735	molecular_function	structural constituent of ribosome
B	0005737	cellular_component	cytoplasm
B	0005840	cellular_component	ribosome
B	0006412	biological_process	translation
B	0022627	cellular_component	cytosolic small ribosomal subunit
B	1990904	cellular_component	ribonucleoprotein complex
C	0003735	molecular_function	structural constituent of ribosome
C	0005840	cellular_component	ribosome
C	0006412	biological_process	translation
C	0022627	cellular_component	cytosolic small ribosomal subunit
C	1990904	cellular_component	ribonucleoprotein complex
D	0003723	molecular_function	RNA binding
D	0003735	molecular_function	structural constituent of ribosome
D	0005634	cellular_component	nucleus
D	0005840	cellular_component	ribosome
D	0022626	cellular_component	cytosolic ribosome
D	0022627	cellular_component	cytosolic small ribosomal subunit
D	1990904	cellular_component	ribonucleoprotein complex
E	0003723	molecular_function	RNA binding
E	0003735	molecular_function	structural constituent of ribosome
E	0005840	cellular_component	ribosome
E	0006412	biological_process	translation
E	0019843	molecular_function	rRNA binding
E	0022627	cellular_component	cytosolic small ribosomal subunit
E	0043232	cellular_component	intracellular non-membrane-bounded organelle
E	1990904	cellular_component	ribonucleoprotein complex
F	0005840	cellular_component	ribosome
F	1990904	cellular_component	ribonucleoprotein complex
G	0005840	cellular_component	ribosome
G	1990904	cellular_component	ribonucleoprotein complex
H	0005840	cellular_component	ribosome
H	0006364	biological_process	rRNA processing
H	0022627	cellular_component	cytosolic small ribosomal subunit
H	0030686	cellular_component	90S preribosome
H	0032040	cellular_component	small-subunit processome
H	0042274	biological_process	ribosomal small subunit biogenesis
H	1990904	cellular_component	ribonucleoprotein complex
I	0005840	cellular_component	ribosome
I	1990904	cellular_component	ribonucleoprotein complex
J	0003735	molecular_function	structural constituent of ribosome
J	0005840	cellular_component	ribosome
J	0019843	molecular_function	rRNA binding
J	0022627	cellular_component	cytosolic small ribosomal subunit
J	0042274	biological_process	ribosomal small subunit biogenesis
J	0043232	cellular_component	intracellular non-membrane-bounded organelle
J	1990904	cellular_component	ribonucleoprotein complex
K	0003723	molecular_function	RNA binding
K	0003735	molecular_function	structural constituent of ribosome
K	0005737	cellular_component	cytoplasm
K	0005840	cellular_component	ribosome
K	0022627	cellular_component	cytosolic small ribosomal subunit
K	1990904	cellular_component	ribonucleoprotein complex
L	0003735	molecular_function	structural constituent of ribosome
L	0005840	cellular_component	ribosome
L	0022627	cellular_component	cytosolic small ribosomal subunit
L	1990904	cellular_component	ribonucleoprotein complex
M	0005840	cellular_component	ribosome
M	1990904	cellular_component	ribonucleoprotein complex
N	0003735	molecular_function	structural constituent of ribosome
N	0005730	cellular_component	nucleolus
N	0005840	cellular_component	ribosome
N	0022627	cellular_component	cytosolic small ribosomal subunit
N	0070181	molecular_function	small ribosomal subunit rRNA binding
N	1990904	cellular_component	ribonucleoprotein complex
O	0005840	cellular_component	ribosome
O	1990904	cellular_component	ribonucleoprotein complex
P	0000028	biological_process	ribosomal small subunit assembly
P	0003735	molecular_function	structural constituent of ribosome
P	0005840	cellular_component	ribosome
P	0022627	cellular_component	cytosolic small ribosomal subunit
P	1990904	cellular_component	ribonucleoprotein complex
Q	0000462	biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
Q	0003723	molecular_function	RNA binding
Q	0003735	molecular_function	structural constituent of ribosome
Q	0005840	cellular_component	ribosome
Q	0022627	cellular_component	cytosolic small ribosomal subunit
Q	1990904	cellular_component	ribonucleoprotein complex
R	0005829	cellular_component	cytosol
R	0005840	cellular_component	ribosome
R	1990904	cellular_component	ribonucleoprotein complex
S	0005829	cellular_component	cytosol
S	0005840	cellular_component	ribosome
S	0015935	cellular_component	small ribosomal subunit
S	1990904	cellular_component	ribonucleoprotein complex
T	0000028	biological_process	ribosomal small subunit assembly
T	0003723	molecular_function	RNA binding
T	0003735	molecular_function	structural constituent of ribosome
T	0005840	cellular_component	ribosome
T	0022627	cellular_component	cytosolic small ribosomal subunit
T	1990904	cellular_component	ribonucleoprotein complex
U	0005840	cellular_component	ribosome
U	1990904	cellular_component	ribonucleoprotein complex
V	0005737	cellular_component	cytoplasm
V	0005840	cellular_component	ribosome
V	0006364	biological_process	rRNA processing
V	1990904	cellular_component	ribonucleoprotein complex
W	0005840	cellular_component	ribosome
W	1990904	cellular_component	ribonucleoprotein complex
X	0005840	cellular_component	ribosome
X	1990904	cellular_component	ribonucleoprotein complex
Y	0005840	cellular_component	ribosome
Y	1990904	cellular_component	ribonucleoprotein complex
Z	0005840	cellular_component	ribosome
Z	1990904	cellular_component	ribonucleoprotein complex
a	0003729	molecular_function	mRNA binding
a	0003735	molecular_function	structural constituent of ribosome
a	0005840	cellular_component	ribosome
a	0022627	cellular_component	cytosolic small ribosomal subunit
a	1990904	cellular_component	ribonucleoprotein complex
b	0005840	cellular_component	ribosome
b	0046872	molecular_function	metal ion binding
b	1990904	cellular_component	ribonucleoprotein complex
c	0000028	biological_process	ribosomal small subunit assembly
c	0003735	molecular_function	structural constituent of ribosome
c	0005840	cellular_component	ribosome
c	0022627	cellular_component	cytosolic small ribosomal subunit
c	0030490	biological_process	maturation of SSU-rRNA
c	1990904	cellular_component	ribonucleoprotein complex
d	0002181	biological_process	cytoplasmic translation
d	0003735	molecular_function	structural constituent of ribosome
d	0005840	cellular_component	ribosome
d	0008270	molecular_function	zinc ion binding
d	0022627	cellular_component	cytosolic small ribosomal subunit
d	1990904	cellular_component	ribonucleoprotein complex
e	0003735	molecular_function	structural constituent of ribosome
e	0005829	cellular_component	cytosol
e	0005840	cellular_component	ribosome
e	0022627	cellular_component	cytosolic small ribosomal subunit
e	1990904	cellular_component	ribonucleoprotein complex
f	0005634	cellular_component	nucleus
f	0005737	cellular_component	cytoplasm
f	0005840	cellular_component	ribosome
f	0046872	molecular_function	metal ion binding
f	1990904	cellular_component	ribonucleoprotein complex
g	0006417	biological_process	regulation of translation
i	0001677	biological_process	formation of translation initiation ternary complex
i	0001731	biological_process	formation of translation preinitiation complex
i	0001732	biological_process	formation of cytoplasmic translation initiation complex
i	0002188	biological_process	translation reinitiation
i	0003725	molecular_function	double-stranded RNA binding
i	0003743	molecular_function	translation initiation factor activity
i	0005515	molecular_function	protein binding
i	0005737	cellular_component	cytoplasm
i	0006412	biological_process	translation
i	0006413	biological_process	translational initiation
i	0010494	cellular_component	cytoplasmic stress granule
i	0016282	cellular_component	eukaryotic 43S preinitiation complex
i	0019901	molecular_function	protein kinase binding
i	0031369	molecular_function	translation initiation factor binding
i	0033290	cellular_component	eukaryotic 48S preinitiation complex
i	0043024	molecular_function	ribosomal small subunit binding
j	0001731	biological_process	formation of translation preinitiation complex
j	0003723	molecular_function	RNA binding
j	0003743	molecular_function	translation initiation factor activity
j	0005515	molecular_function	protein binding
j	0005525	molecular_function	GTP binding
j	0005737	cellular_component	cytoplasm
j	0005829	cellular_component	cytosol
j	0005840	cellular_component	ribosome
j	0005850	cellular_component	eukaryotic translation initiation factor 2 complex
j	0006412	biological_process	translation
j	0006413	biological_process	translational initiation
j	0010494	cellular_component	cytoplasmic stress granule
j	0033290	cellular_component	eukaryotic 48S preinitiation complex
j	0043022	molecular_function	ribosome binding
j	0043614	cellular_component	multi-eIF complex
j	1990856	molecular_function	methionyl-initiator methionine tRNA binding
k	0000049	molecular_function	tRNA binding
k	0001731	biological_process	formation of translation preinitiation complex
k	0003743	molecular_function	translation initiation factor activity
k	0005515	molecular_function	protein binding
k	0005525	molecular_function	GTP binding
k	0005737	cellular_component	cytoplasm
k	0005829	cellular_component	cytosol
k	0005840	cellular_component	ribosome
k	0005850	cellular_component	eukaryotic translation initiation factor 2 complex
k	0006412	biological_process	translation
k	0006413	biological_process	translational initiation
k	0006417	biological_process	regulation of translation
k	0016282	cellular_component	eukaryotic 43S preinitiation complex
k	0016787	molecular_function	hydrolase activity
k	0031369	molecular_function	translation initiation factor binding
k	0033290	cellular_component	eukaryotic 48S preinitiation complex
k	0043614	cellular_component	multi-eIF complex
k	0045903	biological_process	positive regulation of translational fidelity
k	1990606	molecular_function	membrane scission GTPase motor activity
k	1990856	molecular_function	methionyl-initiator methionine tRNA binding
l	0001731	biological_process	formation of translation preinitiation complex
l	0003729	molecular_function	mRNA binding
l	0003743	molecular_function	translation initiation factor activity
l	0005515	molecular_function	protein binding
l	0005525	molecular_function	GTP binding
l	0005737	cellular_component	cytoplasm
l	0005829	cellular_component	cytosol
l	0005840	cellular_component	ribosome
l	0005850	cellular_component	eukaryotic translation initiation factor 2 complex
l	0006412	biological_process	translation
l	0006413	biological_process	translational initiation
l	0008270	molecular_function	zinc ion binding
l	0016282	cellular_component	eukaryotic 43S preinitiation complex
l	0031369	molecular_function	translation initiation factor binding
l	0043614	cellular_component	multi-eIF complex
l	0046872	molecular_function	metal ion binding
l	1990856	molecular_function	methionyl-initiator methionine tRNA binding

Functional Information from PROSITE/UniProt

site_id	PS00052
Number of Residues	27
Details	RIBOSOMAL_S7 Ribosomal protein S7 signature. ErLtnsLMmngrnnGKKlkavrIVkhT

Chain	Residue	Details
F	GLU93-THR119

site_id	PS00053
Number of Residues	18
Details	RIBOSOMAL_S8 Ribosomal protein S8 signature. GyvILTTSaGIMdheeAH

Chain	Residue	Details
W	GLY100-HIS117

site_id	PS00054
Number of Residues	23
Details	RIBOSOMAL_S11 Ribosomal protein S11 signature. LrALarS.GLrIgrieDvTPvPSD

Chain	Residue	Details
O	LEU102-ASP124

site_id	PS00055
Number of Residues	8
Details	RIBOSOMAL_S12 Ribosomal protein S12 signature. KqPNSAiR

Chain	Residue	Details
X	LYS62-ARG69

site_id	PS00056
Number of Residues	13
Details	RIBOSOMAL_S17 Ribosomal protein S17 signature. GDiVtVgQcRPIS

Chain	Residue	Details
L	GLY120-SER132

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KskIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
f	LYS27-ASP52

site_id	PS00323
Number of Residues	25
Details	RIBOSOMAL_S19 Ribosomal protein S19 signature. NGKvfnqveIrpemVGhyLGEFsiT

Chain	Residue	Details
P	ASN98-THR122

site_id	PS00360
Number of Residues	19
Details	RIBOSOMAL_S9 Ribosomal protein S9 signature. GGGhvSQvyAirqAiAKGL

Chain	Residue	Details
Q	GLY71-LEU89

site_id	PS00362
Number of Residues	31
Details	RIBOSOMAL_S15 Ribosomal protein S15 signature. VrkHLernRkDkdAkfrLilIesrihrLarY

Chain	Residue	Details
N	VAL98-TYR128

site_id	PS00528
Number of Residues	15
Details	RIBOSOMAL_S4E Ribosomal protein S4e signature. HlKRLAaPhhWmLdK

Chain	Residue	Details
E	HIS8-LYS22

site_id	PS00529
Number of Residues	23
Details	RIBOSOMAL_S24E Ribosomal protein S24e signature. YGggKStGFGlVYNSvadakkfE

Chain	Residue	Details
Y	TYR64-GLU86

site_id	PS00548
Number of Residues	37
Details	RIBOSOMAL_S3 Ribosomal protein S3 signature. AKsmkfadGfLihSgqpVndfIEtatrhvlLrqGvlG

Chain	Residue	Details
D	ALA147-GLY183

site_id	PS00578
Number of Residues	12
Details	RIBOSOMAL_S6E Ribosomal protein S6e signature. IAGGnDkqGfPM

Chain	Residue	Details
G	ILE52-MET63

site_id	PS00585
Number of Residues	33
Details	RIBOSOMAL_S5 Ribosomal protein S5 signature. GQrtrFkAvvVVGDsn.GhVGlGiktak.EVag.AI

Chain	Residue	Details
C	GLY98-ILE130

site_id	PS00628
Number of Residues	20
Details	RIBOSOMAL_S19E Ribosomal protein S19e signature. PhkhvdaSgsInRkVLQsLE

Chain	Residue	Details
T	PRO90-GLU109

site_id	PS00632
Number of Residues	25
Details	RIBOSOMAL_S4 Ribosomal protein S4 signature. LErRLqtqVYKlglAkSvhhARvLI

Chain	Residue	Details
J	LEU105-ILE129

site_id	PS00646
Number of Residues	14
Details	RIBOSOMAL_S13_1 Ribosomal protein S13 signature. RGiRHfwGlrVRGQ

Chain	Residue	Details
S	ARG123-GLN136

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. AVSAswDkTLRLWNL

Chain	Residue	Details
g	ALA81-LEU95
g	ILE123-THR137
g	ALA218-LEU232

site_id	PS00712
Number of Residues	16
Details	RIBOSOMAL_S17E Ribosomal protein S17e signature. IqSKrLRNkIAGYtTH

Chain	Residue	Details
R	ILE41-HIS56

site_id	PS00733
Number of Residues	8
Details	RIBOSOMAL_S26E Ribosomal protein S26e signature. YCISCAIH

Chain	Residue	Details
a	TYR73-HIS80

site_id	PS00948
Number of Residues	14
Details	RIBOSOMAL_S7E Ribosomal protein S7e signature. KLtRELEKKFQdRH

Chain	Residue	Details
H	LYS76-HIS89

site_id	PS00961
Number of Residues	9
Details	RIBOSOMAL_S28E Ribosomal protein S28e signature. ESEREARrL

Chain	Residue	Details
c	GLU58-LEU66

site_id	PS00962
Number of Residues	12
Details	RIBOSOMAL_S2_1 Ribosomal protein S2 signature 1. AqLLLAARVHLG

Chain	Residue	Details
A	ALA14-GLY25

site_id	PS00963
Number of Residues	25
Details	RIBOSOMAL_S2_2 Ribosomal protein S2 signature 2. PrlVIVtDprsDaqaIkEssyvNIP

Chain	Residue	Details
A	PRO118-PRO142

site_id	PS00996
Number of Residues	9
Details	RIBOSOMAL_S21E Ribosomal protein S21e signature. LYVPRKCSA

Chain	Residue	Details
V	LEU11-ALA19

site_id	PS01168
Number of Residues	33
Details	RIBOSOMAL_S27E Ribosomal protein S27e signature. KCpgClnittvFShAqtavtCesCStvLctPtG

Chain	Residue	Details
b	LYS36-GLY68

site_id	PS01189
Number of Residues	19
Details	RIBOSOMAL_S12E Ribosomal protein S12e signature. ALKvvLrtSlvhDGLarGL

Chain	Residue	Details
M	ALA20-LEU38

site_id	PS01191
Number of Residues	13
Details	RIBOSOMAL_S3AE Ribosomal protein S3Ae signature. LkGRVvEvCLaDL

Chain	Residue	Details
B	LEU61-LEU73

site_id	PS01193
Number of Residues	20
Details	RIBOSOMAL_S8E Ribosomal protein S8e signature. RaaTGAkraqf.RkKrKfELG

Chain	Residue	Details
I	ARG11-GLY30

site_id	PS01262
Number of Residues	23
Details	IF1A Eukaryotic initiation factor 1A signature. MlGnGRveasCfDGnkRmaHIrG

Chain	Residue	Details
i	MET41-GLY63

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	26
Details	ZN_FING: C4-type => ECO:0000255

Chain	Residue	Details
l	CYS236-CYS262
k	ASN249
k	SER284

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:17330950, ECO:0007744\|PubMed:18407956, ECO:0007744\|PubMed:19779198

Chain	Residue	Details
l	SER40
l	SER112
f	LYS27
f	LYS29
f	LYS33
f	LYS63

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:18407956

Chain	Residue	Details
l	THR69
f	GLY76

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:18407956, ECO:0007744\|PubMed:19779198

Chain	Residue	Details
l	SER80

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:19779198

Chain	Residue	Details
l	SER92
l	SER118

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:19779198

Chain	Residue	Details
l	THR116

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)