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- EMDB-19541: Structure of a yeast 48S-AUC preinitiation complex in closed conf... -

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Basic information

Entry
Database: EMDB / ID: EMD-19541
TitleStructure of a yeast 48S-AUC preinitiation complex in closed conformation
Map dataFor optimal visualization of eIF2 domains (eIFalpha, eIF2gamma and N-terminal domain of eIF2beta), gauss-filter the map by 1.34 and display it at 0.015 contour level, respectively.
Sample
  • Complex: Structure of a yeast 48S-AUC preinitiation complex in closed conformation
    • Complex: Ribosome
      • RNA: x 1 types
      • Protein or peptide: x 33 types
    • Complex: tRNA
      • RNA: x 1 types
    • Complex: Initiation factors eIF1A
      • Protein or peptide: x 1 types
    • Complex: Initiation factor eIF2
      • Protein or peptide: x 3 types
    • Complex: mRNA
      • RNA: x 1 types
  • Protein or peptide: x 1 types
  • Ligand: x 5 types
Keywordsribosome / translation / initiation factors / 40S / eIF1A / AUC codon / eIF2 / tRNAi / 48S PIC / small ribosome subunit
Function / homology
Function and homology information


formation of translation initiation ternary complex / Recycling of eIF2:GDP / Cellular response to mitochondrial stress / ABC-family proteins mediated transport / methionyl-initiator methionine tRNA binding / translation reinitiation / eukaryotic translation initiation factor 2 complex / formation of cytoplasmic translation initiation complex / multi-eIF complex / protein-synthesizing GTPase ...formation of translation initiation ternary complex / Recycling of eIF2:GDP / Cellular response to mitochondrial stress / ABC-family proteins mediated transport / methionyl-initiator methionine tRNA binding / translation reinitiation / eukaryotic translation initiation factor 2 complex / formation of cytoplasmic translation initiation complex / multi-eIF complex / protein-synthesizing GTPase / eukaryotic 43S preinitiation complex / formation of translation preinitiation complex / eukaryotic 48S preinitiation complex / positive regulation of translational fidelity / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression / ribosomal small subunit binding / 90S preribosome / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation initiation factor binding / translation initiation factor activity / rescue of stalled ribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / translational initiation / protein kinase C binding / positive regulation of apoptotic signaling pathway / modification-dependent protein catabolic process / cytoplasmic stress granule / protein tag activity / rRNA processing / ribosomal small subunit biogenesis / double-stranded RNA binding / small ribosomal subunit rRNA binding / ribosome binding / ribosomal small subunit assembly / small ribosomal subunit / cytosolic small ribosomal subunit / cytoplasmic translation / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / ribonucleoprotein complex / positive regulation of protein phosphorylation / translation / GTPase activity / mRNA binding / ubiquitin protein ligase binding / GTP binding / nucleolus / protein kinase binding / RNA binding / zinc ion binding / nucleus / metal ion binding / cytosol / cytoplasm
Similarity search - Function
Translation initiation factor IF2/IF5 domain / Translation initiation factor IF2/IF5, N-terminal / Translation initiation factor IF2/IF5, zinc-binding / Translation initiation factor IF2/IF5 / Domain found in IF2B/IF5 / domain present in translation initiation factor eIF2B and eIF5 / Translation initiation factor 1A (eIF-1A), conserved site / Eukaryotic initiation factor 1A signature. / eukaryotic translation initiation factor 1A / Translation initiation factor 1A (eIF-1A) ...Translation initiation factor IF2/IF5 domain / Translation initiation factor IF2/IF5, N-terminal / Translation initiation factor IF2/IF5, zinc-binding / Translation initiation factor IF2/IF5 / Domain found in IF2B/IF5 / domain present in translation initiation factor eIF2B and eIF5 / Translation initiation factor 1A (eIF-1A), conserved site / Eukaryotic initiation factor 1A signature. / eukaryotic translation initiation factor 1A / Translation initiation factor 1A (eIF-1A) / Translation initiation factor 2, alpha subunit / Translation initiation factor 2, alpha subunit, middle domain superfamily / Translation initiation factor 2, alpha subunit, C-terminal / IF2a, S1-like domain / Eukaryotic translation initiation factor 2 alpha subunit / Initiation factor eIF2 gamma, C-terminal / Initiation factor eIF2 gamma, domain 2 / Initiation factor eIF2 gamma, GTP-binding domain / Initiation factor eIF2 gamma, C terminal / : / RNA-binding domain, S1, IF1 type / Translation initiation factor 1A / IF-1 / S1 domain IF1 type profile. / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / S1 domain profile. / : / Ribosomal protein S26e signature. / Ribosomal protein S26e / Ribosomal protein S21e, conserved site / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S21e signature. / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / Small (40S) ribosomal subunit Asc1/RACK1 / S27a-like superfamily / 40S Ribosomal protein S10 / Ribosomal protein S10, eukaryotic/archaeal / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / Ribosomal protein S30 / : / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S7e signature. / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S19e / Translation elongation factor EFTu-like, domain 2 / Ribosomal protein S3Ae, conserved site / Ribosomal protein S19e / Ribosomal protein S3Ae signature. / Ribosomal_S19e / Ribosomal protein S27e signature. / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / Ribosomal protein S19A/S15e / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / : / Ribosomal protein S6, eukaryotic / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S4e, N-terminal / RS4NT (NUC023) domain / 40S ribosomal protein S11, N-terminal / Ribosomal_S17 N-terminal / Ribosomal protein S7e / Ribosomal protein S7e / Ribosomal protein S4, KOW domain / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal family S4e / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S6e signature. / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein S24e
Similarity search - Domain/homology
KLLA0B11231p / Eukaryotic translation initiation factor 2 subunit beta / Eukaryotic translation initiation factor 2 subunit alpha / Small ribosomal subunit protein uS11 / Eukaryotic translation initiation factor 2 subunit gamma / Small ribosomal subunit protein eS28 / Eukaryotic translation initiation factor 1A / Ubiquitin-ribosomal protein eS31 fusion protein / KLLA0F25542p / KLLA0F18040p ...KLLA0B11231p / Eukaryotic translation initiation factor 2 subunit beta / Eukaryotic translation initiation factor 2 subunit alpha / Small ribosomal subunit protein uS11 / Eukaryotic translation initiation factor 2 subunit gamma / Small ribosomal subunit protein eS28 / Eukaryotic translation initiation factor 1A / Ubiquitin-ribosomal protein eS31 fusion protein / KLLA0F25542p / KLLA0F18040p / KLLA0F09812p / KLLA0F07843p / 40S ribosomal protein S12 / Small ribosomal subunit protein eS6 / KLLA0E23673p / 40S ribosomal protein S8 / Small ribosomal subunit protein uS2 / KLLA0E12277p / 40S ribosomal protein S27 / Small ribosomal subunit protein uS14 / KLLA0D10659p / 40S ribosomal protein S3 / 40S ribosomal protein S26 / 40S ribosomal protein S7 / 40S ribosomal protein S24 / 40S ribosomal protein S30 / KLLA0B08173p / Small ribosomal subunit protein uS8 / 40S ribosomal protein S25 / Small ribosomal subunit protein eS1 / 40S ribosomal protein S4 / KLLA0B01562p / KLLA0B01474p / KLLA0A10483p / KLLA0A07194p / Small ribosomal subunit protein eS21 / Small ribosomal subunit protein uS9
Similarity search - Component
Biological speciesKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast) / Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) / Kluyveromyces lactis (yeast) / Kluyveromyces lactis NRRL Y-1140 (yeast) / Saccharomyces cerevisiae S288C (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.35 Å
AuthorsVillamayor-Belinchon L / Sharma P / Llacer JL / Hussain T
Funding support Spain, India, 2 items
OrganizationGrant numberCountry
Ministerio de Ciencia e Innovacion (MCIN)PID2020-116880GB-I00 Spain
Department of Biotechnology (DBT, India)IA/I/17/2/503313 India
CitationJournal: Nucleic Acids Res / Year: 2024
Title: Structural basis of AUC codon discrimination during translation initiation in yeast.
Authors: Laura Villamayor-Belinchón / Prafful Sharma / Yuliya Gordiyenko / Jose L Llácer / Tanweer Hussain /
Abstract: In eukaryotic translation initiation, the 48S preinitiation complex (PIC) scans the 5' untranslated region of mRNAs to search for the cognate start codon (AUG) with assistance from various ...In eukaryotic translation initiation, the 48S preinitiation complex (PIC) scans the 5' untranslated region of mRNAs to search for the cognate start codon (AUG) with assistance from various eukaryotic initiation factors (eIFs). Cognate start codon recognition is precise, rejecting near-cognate codons with a single base difference. However, the structural basis of discrimination of near-cognate start codons was not known. We have captured multiple yeast 48S PICs with a near-cognate AUC codon at the P-site, revealing that the AUC codon induces instability in the codon-anticodon at the P-site, leading to a disordered N-terminal tail of eIF1A. Following eIF1 dissociation, the N-terminal domain of eIF5 fails to occupy the vacant eIF1 position, and eIF2β becomes flexible. Consequently, 48S with an AUC codon is less favourable for initiation. Furthermore, we observe hitherto unreported metastable states of the eIF2-GTP-Met-tRNAMet ternary complex, where the eIF2β helix-turn-helix domain may facilitate eIF5 association by preventing eIF1 rebinding to 48S PIC. Finally, a swivelled head conformation of 48S PIC appears crucial for discriminating incorrect and selection of the correct codon-anticodon pair during translation initiation.
History
DepositionFeb 2, 2024-
Header (metadata) releaseSep 11, 2024-
Map releaseSep 11, 2024-
UpdateOct 23, 2024-
Current statusOct 23, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_19541.png

Downloads & links

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Map

FileDownload / File: emd_19541.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationFor optimal visualization of eIF2 domains (eIFalpha, eIF2gamma and N-terminal domain of eIF2beta), gauss-filter the map by 1.34 and display it at 0.015 contour level, respectively.
Projections & slices

Image control

Size
Brightness
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.34 Å/pix.
x 300 pix.
= 402. Å		1.34 Å/pix.
x 300 pix.
= 402. Å		1.34 Å/pix.
x 300 pix.
= 402. Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.34 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.07
Minimum - Maximum-0.474731 - 0.86769843
Average (Standard dev.)0.000105236635 (±0.03691302)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 402.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_19541_msk_1.map
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Half map: #2

Fileemd_19541_half_map_1.map
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Half map: #1

Fileemd_19541_half_map_2.map
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Sample components

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Entire : Structure of a yeast 48S-AUC preinitiation complex in closed conf...

EntireName: Structure of a yeast 48S-AUC preinitiation complex in closed conformation
Components
  • Complex: Structure of a yeast 48S-AUC preinitiation complex in closed conformation
    • Complex: Ribosome
      • RNA: 18S ribosomal RNA
      • Protein or peptide: Small ribosomal subunit protein uS2
      • Protein or peptide: Small ribosomal subunit protein eS1
      • Protein or peptide: Small ribosomal subunit protein uS5
      • Protein or peptide: 40S ribosomal protein S4
      • Protein or peptide: Small ribosomal subunit protein eS6
      • Protein or peptide: 40S ribosomal protein S7
      • Protein or peptide: 40S ribosomal protein S8
      • Protein or peptide: KLLA0E23673p
      • Protein or peptide: KLLA0A10483p
      • Protein or peptide: KLLA0F18040p
      • Protein or peptide: Small ribosomal subunit protein uS11
      • Protein or peptide: 40S ribosomal protein S21
      • Protein or peptide: Small ribosomal subunit protein uS8
      • Protein or peptide: KLLA0B11231p
      • Protein or peptide: 40S ribosomal protein S24
      • Protein or peptide: 40S ribosomal protein S27
      • Protein or peptide: 40S ribosomal protein S30
      • Protein or peptide: 40S ribosomal protein L41-A
      • Protein or peptide: 40S ribosomal protein S3
      • Protein or peptide: KLLA0D10659p
      • Protein or peptide: KLLA0B08173p
      • Protein or peptide: 40S ribosomal protein S12
      • Protein or peptide: KLLA0F07843p
      • Protein or peptide: Small ribosomal subunit protein uS9
      • Protein or peptide: KLLA0B01474p
      • Protein or peptide: KLLA0B01562p
      • Protein or peptide: KLLA0A07194p
      • Protein or peptide: Small ribosomal subunit protein uS10
      • Protein or peptide: 40S ribosomal protein S25
      • Protein or peptide: Small ribosomal subunit protein eS28
      • Protein or peptide: Small ribosomal subunit protein uS14
      • Protein or peptide: Small ribosomal subunit protein eS31
      • Protein or peptide: KLLA0E12277p
    • Complex: tRNA
      • RNA: Met-tRNAi
    • Complex: Initiation factors eIF1A
      • Protein or peptide: Eukaryotic translation initiation factor 1A
    • Complex: Initiation factor eIF2
      • Protein or peptide: Eukaryotic translation initiation factor 2 subunit alpha
      • Protein or peptide: Eukaryotic translation initiation factor 2 subunit gamma
      • Protein or peptide: Eukaryotic translation initiation factor 2 subunit beta
    • Complex: mRNA
      • RNA: mRNA (5'-R(P*AP*AP*U)-3')
  • Protein or peptide: 40S ribosomal protein S26
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
  • Ligand: METHIONINE
  • Ligand: water

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Supramolecule #1: Structure of a yeast 48S-AUC preinitiation complex in closed conf...

SupramoleculeName: Structure of a yeast 48S-AUC preinitiation complex in closed conformation
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#16, #18-#41
Molecular weightTheoretical: 1.8 MDa

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Supramolecule #2: Ribosome

SupramoleculeName: Ribosome / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#16, #18-#35
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)

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Supramolecule #3: tRNA

SupramoleculeName: tRNA / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #38
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)

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Supramolecule #4: Initiation factors eIF1A

SupramoleculeName: Initiation factors eIF1A / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #36
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)

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Supramolecule #5: Initiation factor eIF2

SupramoleculeName: Initiation factor eIF2 / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #39-#41
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)

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Supramolecule #6: mRNA

SupramoleculeName: mRNA / type: complex / ID: 6 / Parent: 1 / Macromolecule list: #37
Source (natural)Organism: Kluyveromyces lactis (yeast)

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Macromolecule #1: 18S ribosomal RNA

MacromoleculeName: 18S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 579.454875 KDa
SequenceString: UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA (PSU)UUGAUAGUU CCUUUACUAC AUGGAUAUCU GUGGUAA UU CUAGAGCUAA ...String:
UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA (PSU)UUGAUAGUU CCUUUACUAC AUGGAUAUCU GUGGUAA UU CUAGAGCUAA UACAUGCUUA AAAUCUCGAC CCUUUGGAAG AGAUGUAUUU AUUAGAUAAA AAAUCAAUGU CUUCGGAC U CCUUGAUGAU UCAUAAUAAC UUUUCGAAUC GCAUGGCCUU GUGCUGGCGA UGGUUCAUUC AAAUUUCUGC CCUAUCAAC UUUCGAUGGU AGGAUAGUGG CCUACCAUGG UUUCAACGGG UAACGGGGAA UAAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAACGGCU ACCACAUCCA AGGAAGGCAG CAGGCGCGCA AAUUACCCAA UCCUAAUUCA GGGAGGUAG(PSU) GACAAUA AA UAACGAUACA GGGCCCAUUC GGGUCUUGUA AUUGGAAUGA GUACAAUGUA AAUACCUUAA CGAGGAACAA CUGGAGGG C AAGUCUGGUG CCAGCAGCCG CGGUAAUUCC AGCUCCAGUA GCGUAUAUUA AAGUUGUUGC AGUUAAAAAG CUCGUAGUU GAACUUUGGG UCUGGUUGUC CGGUCCGACU UUAUGUCGCG CACUGGUUUU CAACCGGAUC UUUCCUUCUG GCUAACCUGU ACUCCUUGU GGGUGCAGGC GAACCAGGAC UUUUACUUUG AAAAAAUUAG AGUG(PSU)UCAAA GCAGGCGAAA GCUCGAA UA UAUUAGCAUG GAAUAAUGGA AUAGGACGUU UGGUUCUAUU UUGUUGGUUU CUAGGACCAU CGUAAUGAUU AAUAGGGA C GGUCGGGGGC AUCAGUAUUC AAUUGUCAGA GGUGAAAUUC UUGGAUUUAU UGAAGACUAA CUACUGCGAA AGCAUUUGC CAAGGACGUU UUCAUUAAUC AAGAACGAAA GUUAGGGGAU CGAAGAUGA(PSU) CAGAUAC(5MC)GU CGUAGUCUUA AC CAUAAAC UAUGCCGACU AGGGAUCGGG UGGUGUUUUU CUUAUGACCC ACUCGGCACC UUACGAGAAA UCAAAGUCUU UGG GUUCUG GGGGGAGUAU GGUCGCAAGG CUGAAACUUA AAGGAAUUGA CGGAAGGGCA CCACCAGGAG UGGAGCCUGC GGCU UAAUU UGAC(B8N)CAACA CGGGGAAACU CACCAGGUCC AGACACAAUA AGGAUUGACA GAUUGAGAGC UCUUUCUUGA U UUUGUGGG UGGUGGUGCA UGGCCGUUCU UAGU(PSU)GGUGG AGUGAUUUGU CUGCUUAAUU GCGAUAACGA ACGAGACC U UAACCUACUA AAUAGGGUUG CUGGCACUUG CCGGUUGACU CUUCUUAGAG GGACUAUCGG UUUCAAGCCG AUGGAAGUU UGAGGCAAUA ACA(2MG)GUCUGU GAUGCCCUUA GACGUUCUGG GCCGCACGCG CGCUACACUG ACGGAGCCAG CGAGUA CAA CCUUGGCCGA GAGGUCUGGG UAAUCUUGUG AAACUCCGUC GUGCUGGGGA UAGAGCAUUG UAAUUAUUGC UCUUCAA C(2MG) AG(7MG)AAUUCCU AGUAAGCGCA AGUCAUCAGC UUGCGUUGAU UACGUCCCUG CCCUUUGUAC ACACCG (5MC)CC GUCGCUAGUA CCGAUUGAAU GGCUUAGUGA GGCCUCAGGA UUUGCUUAGA GAAGGGGGCA ACUCCAUCUC AGA GCGAAG AAUCUGGUCA AACUUGGUCA UUUAGAGGAA CUAAAAGUCG UAACAAGGUU UCCGUAGGUG A(MA6)CCUGCGGA AGGAUCAUU A

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Macromolecule #37: mRNA (5'-R(P*AP*AP*U)-3')

MacromoleculeName: mRNA (5'-R(P*AP*AP*U)-3') / type: rna / ID: 37 / Number of copies: 1
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 15.30494 KDa
SequenceString:
GGGCUCUCUU CUCUCUCUAA CUAUAAAAAU CUCUCUUCUC UCUCUCGAU

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Macromolecule #38: Met-tRNAi

MacromoleculeName: Met-tRNAi / type: rna / ID: 38 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 24.713969 KDa
SequenceString:
AGCGCCGU(1MG)(2MG) CGCAG(H2U)GGAA GCGC(M2G)CAGGG CUCAU(T6A)ACCC UGAU(7MG)(H2U)(5MC) (5MC)U CGGAUCG(1MA)AA CCG(RIA)GCGGCG CUACCA

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Macromolecule #2: Small ribosomal subunit protein uS2

MacromoleculeName: Small ribosomal subunit protein uS2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: ATCC 8585
Molecular weightTheoretical: 28.264525 KDa
SequenceString: MSLPSTFDLT SEDAQLLLAA RVHLGAKNVQ VHQEPYVYKA RPDGVNVINV GKTWEKIVLA ARIIAAIPNP EDVVAISSRT YGQRAVLKY AAHTGATPIA GRFTPGSFTN YITRSFKEPR LVIVTDPRSD AQAIKESSYV NIPVIALTDL DSPSEYVDVA I PCNNRGKH ...String:
MSLPSTFDLT SEDAQLLLAA RVHLGAKNVQ VHQEPYVYKA RPDGVNVINV GKTWEKIVLA ARIIAAIPNP EDVVAISSRT YGQRAVLKY AAHTGATPIA GRFTPGSFTN YITRSFKEPR LVIVTDPRSD AQAIKESSYV NIPVIALTDL DSPSEYVDVA I PCNNRGKH SIGLIWYLLA REVLRLRGAL PDRTQPWAIM PDLYFYRNPE EIEQQTAEEE AVASGEQTEE AVDATEEQTE AA EWAEEGQ AQEEEWN

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #3: Small ribosomal subunit protein eS1

MacromoleculeName: Small ribosomal subunit protein eS1 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 28.971643 KDa
SequenceString: MAVGKNKRLS KGKKGLKKRV VDPFTRKEWY DIKAPSTFEN RNVGKTLVNK SVGLKNASDS LKGRVVEVCL ADLQGSEDHS FRKVKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRTSYAQSS H IRQIRKVI ...String:
MAVGKNKRLS KGKKGLKKRV VDPFTRKEWY DIKAPSTFEN RNVGKTLVNK SVGLKNASDS LKGRVVEVCL ADLQGSEDHS FRKVKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRTSYAQSS H IRQIRKVI SEILTREVQN STLAQLTSKL IPEVINKEIE NATKDIFPLQ NVHIRKVKLL KQPKFDLGSL LSLHGEASAE EK GKKVAGF KDEILETV

UniProtKB: Small ribosomal subunit protein eS1

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Macromolecule #4: Small ribosomal subunit protein uS5

MacromoleculeName: Small ribosomal subunit protein uS5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 27.649979 KDa
SequenceString: MSAPQAQGQQ APRRGGFGGA NRGGRGGRRG GRRDQEEKGW VPVTKLGRLV KAGKISSIEE IFLHSLPVKE FQIIDQLLPN LKDEVMNIK PVQKQTRAGQ RTRFKAVVVV GDSNGHVGLG IKTAKEVAGA IRAGIIIAKL SVIPIRRGYW GTNLGQPHSL A TKTSGKCG ...String:
MSAPQAQGQQ APRRGGFGGA NRGGRGGRRG GRRDQEEKGW VPVTKLGRLV KAGKISSIEE IFLHSLPVKE FQIIDQLLPN LKDEVMNIK PVQKQTRAGQ RTRFKAVVVV GDSNGHVGLG IKTAKEVAGA IRAGIIIAKL SVIPIRRGYW GTNLGQPHSL A TKTSGKCG SVSVRLIPAP RGSGIVASPA VKKLMQLAGV EDVYTSSTGS TRTLENTLKA AFVAIGNTYG FLTPNLWEVQ AL TPSPMDV YADYATASKK KL

UniProtKB: KLLA0F09812p

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Macromolecule #5: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 29.617514 KDa
SequenceString: MARGPKKHLK RLAAPHHWML DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TFPAGFMDV ITLEATNENF RLVYDVKGRF AVHRITDEEA SYKLAKVKKV QLGKKGIPYV VTHDGRTIRY PDPNIKVNDT V KVDLATGT ...String:
MARGPKKHLK RLAAPHHWML DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TFPAGFMDV ITLEATNENF RLVYDVKGRF AVHRITDEEA SYKLAKVKKV QLGKKGIPYV VTHDGRTIRY PDPNIKVNDT V KVDLATGT ITDFIKFDTG KLVYVTGGRN LGRVGTIVHR ERHEGGFDLV HIKDSLENTF VTRLNNVFVI GEPGRPWISL PK GKGIKLT ISEERDRRRA QHGL

UniProtKB: 40S ribosomal protein S4

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Macromolecule #6: Small ribosomal subunit protein eS6

MacromoleculeName: Small ribosomal subunit protein eS6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 26.970391 KDa
SequenceString: MKLNISYPIN GTQKCIEIDD EHRVRVFYDK RIGQEVDGES VGDEFKGYVF KIAGGNDKQG FPMKQGVLLP TRVKLLLAKG HSCYRPRRN GERKRKSVRG AIVGPDLAVL ALIITKKGEQ EIEGITNDTV PKRLGPKRAN NIRKFFGLTK EDDVRDYVIR R EVTKGDKS ...String:
MKLNISYPIN GTQKCIEIDD EHRVRVFYDK RIGQEVDGES VGDEFKGYVF KIAGGNDKQG FPMKQGVLLP TRVKLLLAKG HSCYRPRRN GERKRKSVRG AIVGPDLAVL ALIITKKGEQ EIEGITNDTV PKRLGPKRAN NIRKFFGLTK EDDVRDYVIR R EVTKGDKS YTKAPKIQRL VTPQRLQRKR QQKSLKIKNA QAQREAAAEY AQLLAKRLSE RKAEKAEVRK RRASSLKA

UniProtKB: Small ribosomal subunit protein eS6

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Macromolecule #7: 40S ribosomal protein S7

MacromoleculeName: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 21.735297 KDa
SequenceString:
MSDPQAKILS QAPTELELQV AQAFIDLENN SPELKADLRA LQFKSIREIE VAGGKKALAV FVPVPSLAAY HKVQIKLTRE LEKKFQDRH VIFLAERRIL PKPSRKSRQT QKRPRSRTLT AVHDKILEDL VFPTEIVGKR VRYLVGGNKI QKILLNSKDV Q HIDNKLES FQAVYNKLTG KQIVFEIPSE TH

UniProtKB: 40S ribosomal protein S7

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Macromolecule #8: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 22.642727 KDa
SequenceString: MGISRDSRHK RAATGAKRAQ FRKKRKFELG RQAANTKIGT KRIHPVRTRG GNQKFRALRI ETGNFSWASE GVARKTRITG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWYESHYGQ SLGKKKNTKA EEETATTSKN TERKWAARAA EAKIEHAVDS Q FGAGRLYA ...String:
MGISRDSRHK RAATGAKRAQ FRKKRKFELG RQAANTKIGT KRIHPVRTRG GNQKFRALRI ETGNFSWASE GVARKTRITG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWYESHYGQ SLGKKKNTKA EEETATTSKN TERKWAARAA EAKIEHAVDS Q FGAGRLYA AISSRPGQSG RCDGYILEGE ELAFYLRRLT AKK

UniProtKB: 40S ribosomal protein S8

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Macromolecule #9: KLLA0E23673p

MacromoleculeName: KLLA0E23673p / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 21.587049 KDa
SequenceString:
MPRAPRTYSK TYSTPKRPYE SARLDAELKL AGEYGLKNKR EIYRISFQLS KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRIGVLSED KKKLDYVLAL KVEDFLERRL QTQVYKLGLA KSVHHARVLI SQRHIAVGKQ IVNIPSFMVR LESEKHIDFA R TSPFGGAR PGRVARKRAA AAGGEEADEE

UniProtKB: KLLA0E23673p

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Macromolecule #10: KLLA0A10483p

MacromoleculeName: KLLA0A10483p / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 17.84393 KDa
SequenceString:
MSTELTVQSE RAFQKQPHIF TNPKAKANRK TKRWYKNVGL GFKTPKTAIE GSYIDKKCPF TGLVSIRGKI LTGTVVSTRM HRTIVIRRD YLHYVPKYNR YEKRHKNVPA HVSPAFRVQV GDIVTVGQCR PISKTVRFNV LKVASATGKA NKQFAKF

UniProtKB: KLLA0A10483p

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Macromolecule #11: KLLA0F18040p

MacromoleculeName: KLLA0F18040p / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 16.989875 KDa
SequenceString:
MGRMHSKGKG MSSSAIPYSR NAPAWFKGSS DGVVEQIIKY ARKGLTPSQI GVLLRDAHGV TQAKVITGNK ILRILKSNGL APEIPEDLY FLIKKAVSVR KHLERNRKDK DAKFRLILIE SRIHRLARYY RTVSVLPPNW KYESATASAL VN

UniProtKB: KLLA0F18040p

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Macromolecule #12: Small ribosomal subunit protein uS11

MacromoleculeName: Small ribosomal subunit protein uS11 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 14.530655 KDa
SequenceString:
MANVVQAKDN SQVFGVARIF ASFNDTFVHV TDLSGRETIA RVTGGMKVKA DRDESSPYAA MLAAQDVAAK CKEVGITAVH IKIRATGGT RSKTPGPGGQ AALRALARSG LRIGRIEDVT PVPSDSTRKK GGRRGRRL

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #13: 40S ribosomal protein S21

MacromoleculeName: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 9.797949 KDa
SequenceString:
MENDKGQLVE LYVPRKCSAT NRIIKAKDHS SVQINIAQVD EEGRAIPGEY VTYALSGYIR ARGEADDSLN RLAQQDGLLK NVWSYSR

UniProtKB: Small ribosomal subunit protein eS21

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Macromolecule #14: Small ribosomal subunit protein uS8

MacromoleculeName: Small ribosomal subunit protein uS8 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 14.645041 KDa
SequenceString:
MTRTSVLADA LNAINNAEKT GKRQVLIRPS SKVIIKFLQV MQKHGYIGEF EYIDDHRSGK IVVQLNGRLN KCGVISPRFN VKIADVEKW TANLLPARQF GYVILTTSAG IMDHEEAHRK HVSGKILGFV Y

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #15: KLLA0B11231p

MacromoleculeName: KLLA0B11231p / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 16.047897 KDa
SequenceString:
MGKGKPRGLN SARKLRVHRR NNRWAETTYK KRLLGTAFKS SPFGGSSHAK GIVLEKIGIE SKQPNSAIRK CVRVQLIKNG KKVTAFVPN DGCLNFVDEN DEVLLAGFGR KGKAKGDIPG VRFKVVKVSG VSLLALWKEK KEKPRS

UniProtKB: KLLA0B11231p

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Macromolecule #16: 40S ribosomal protein S24

MacromoleculeName: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 15.194549 KDa
SequenceString:
MSDAITIRTR KVISNPLLAR KQFVVDVLHP NRANVSKDEL REKLAEAYKA EKDAVSVFGF RTQYGGGKST GFGLVYNSVA DAKKFEPAY RLVRYGLAEK VEKASRQQRK QRKNRGKKIF GTGKSIAKKA ARRNAD

UniProtKB: 40S ribosomal protein S24

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Macromolecule #17: 40S ribosomal protein S26

MacromoleculeName: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 13.539957 KDa
SequenceString:
MPKKRASNGR NKKGRGHVKP VRCVNCSRSV PKDKAIKRMA IRNIVEAAAI RDLSEASVYA EYALPKTYNK LHYCISCAIH ARIVRVRSR TDRRIRAPPQ RPRFNRDNKV SPADAAKKAL

UniProtKB: 40S ribosomal protein S26

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Macromolecule #18: 40S ribosomal protein S27

MacromoleculeName: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 8.884362 KDa
SequenceString:
MVLVQDLLHP TAASEARKHK LKTLVQSPRS HFLDVKCPGC LNITTVFSHA QTAVTCESCS TVLCTPTGGK AKLSEGTSFR RK

UniProtKB: 40S ribosomal protein S27

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Macromolecule #19: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 7.141421 KDa
SequenceString:
MGKVHGSLAR AGKVKSQTPK VEKQEKPKQP KGRAYKRLLY TRRFVNVTLT NGKRKMNPSP SSQ

UniProtKB: 40S ribosomal protein S30

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Macromolecule #20: 40S ribosomal protein L41-A

MacromoleculeName: 40S ribosomal protein L41-A / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 3.354243 KDa
SequenceString:
MRAKWRKKRT RRLKRKRRKV RARSK

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Macromolecule #21: 40S ribosomal protein S3

MacromoleculeName: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 26.300535 KDa
SequenceString: MVAIISKKRK LVADGVFYAE LNEFFTRELA EEGYSGVEVR VTPTKTEIII RATKVQDVVG ENGRRINELT LLIEKRFKYK RGTIALYAE RVHDRGLSAV AQAESMKFKL LNGLAIRRAA YGVVRYVMES GAKGCEVVIS GKLRAARAKS MKFADGFLIH S GQPVNDFI ...String:
MVAIISKKRK LVADGVFYAE LNEFFTRELA EEGYSGVEVR VTPTKTEIII RATKVQDVVG ENGRRINELT LLIEKRFKYK RGTIALYAE RVHDRGLSAV AQAESMKFKL LNGLAIRRAA YGVVRYVMES GAKGCEVVIS GKLRAARAKS MKFADGFLIH S GQPVNDFI ETATRHVLLR QGVLGIKVKI MKDPSRNTSG PKALPDAVTI IEPKEEEPVL EPSVKDYRPT EPVEAAESA

UniProtKB: 40S ribosomal protein S3

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Macromolecule #22: KLLA0D10659p

MacromoleculeName: KLLA0D10659p / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 25.385975 KDa
SequenceString: MSEHEAQVEV EVQEDFEVVQ EFVPVELATT IPVEIQQAQQ EIKLFNKWSF EDVEVKDASL VDYIQISKPI YVAHTAGRYA NKRFRKAQC PIVERLTNSL MMNGRNNGKK LKAVRIVKHT LEIINVLTDQ NPLQVVVDAI INSGPREDTT RVGGGGAARR Q AVDVSPLR ...String:
MSEHEAQVEV EVQEDFEVVQ EFVPVELATT IPVEIQQAQQ EIKLFNKWSF EDVEVKDASL VDYIQISKPI YVAHTAGRYA NKRFRKAQC PIVERLTNSL MMNGRNNGKK LKAVRIVKHT LEIINVLTDQ NPLQVVVDAI INSGPREDTT RVGGGGAARR Q AVDVSPLR RVNQSIALLT IGAREAAFRN IKTIAETLAE ELINAAKGSS TSYAIKKKDE LERVAKSNR

UniProtKB: KLLA0D10659p

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Macromolecule #23: KLLA0B08173p

MacromoleculeName: KLLA0B08173p / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 12.584377 KDa
SequenceString:
MLIPKEDRKK IYQHLFQEGV LVAKKDFNQP KHEEIDTKNL FVIKALQSLT SKGFVKTQFS WQYYYYTLTE EGVVYLREYL NLPEHIFPA TYLAGQSGDQ RPQGKKY

UniProtKB: KLLA0B08173p

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Macromolecule #24: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 14.466398 KDa
SequenceString:
MSDVEEVQQV PVAELTIEDA LKVVLRTSLV HDGLARGLRE SAKALTRGEG QLAVLVESVT EEAISKLVQG LATENNVPLI KVADAKQLG EWAGLGKIDR DGNARKVVGA SVVVVKNWGA DTQEREILLE HFSQQ

UniProtKB: 40S ribosomal protein S12

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Macromolecule #25: KLLA0F07843p

MacromoleculeName: KLLA0F07843p / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 15.986796 KDa
SequenceString:
MSEAAAPRKR SFKTYSYKGV DLEKLLEMPT EDFVKLAPAR VRRKFARGLS EKPAGLMKKL RAAKLSAPEN EKPAVVRTHL RNMIIVPEM IGSVVGVYNG KVFNQVEIRP EMVGHYLGEF SITYTPVRHG RAGATTSRFI PLR

UniProtKB: KLLA0F07843p

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Macromolecule #26: Small ribosomal subunit protein uS9

MacromoleculeName: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 15.874531 KDa
SequenceString:
MSTVPSVQTF GKKKSATAVA HVKAGKGLIK VNGSPITLVQ PEILRFKVYE PLLLVGLDKF ANIDIRVKVT GGGHVSQVYA IRQAIAKGL VAYHQKFVDE QSKNELKKAF TSYDRTLLIA DSRRPEPKKF GGRGARSRFQ KSYR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #27: KLLA0B01474p

MacromoleculeName: KLLA0B01474p / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 15.722216 KDa
SequenceString:
MGRVRTKTVK RASKALIEKY YPKLTMDFQT NKRLCDEIAT IQSKRLRNKI AGYTTHLMKR IQKGPVRGIS FKLQEEERER KDQYVPDVS ALDLSHSNDV LNVDTQTAEL VNSLGLKLPL SVSSVSAVRD RRFRKRN

UniProtKB: KLLA0B01474p

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Macromolecule #28: KLLA0B01562p

MacromoleculeName: KLLA0B01562p / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 17.084602 KDa
SequenceString:
MSLVVQEQGS FQHILRLLNT NVDGNINVVY ALTTIRGVGR RYANLVCKKA DVDLHKRAGE LTQEELERIV QIMQNPTHYK IPAWFLNRQ KDVNDGKDYH SLANNLESKL RDDLERLKKI RSHRGIRHFW GLRVRGQHTK TTGRRRA

UniProtKB: KLLA0B01562p

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Macromolecule #29: KLLA0A07194p

MacromoleculeName: KLLA0A07194p / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 15.87901 KDa
SequenceString:
MPGVSVRDVP AQDFINNYAS FLQRQGKLEV PGYVDIVKTS AGNELPPQDS EGWFYKRAAS VARHIYLRKQ VGVGKLNKLY GGAKNRGVR PHKHVDASGS INRKVLQSLE KLGVVEISPK GGRRISDNGL RDLDRIAAAT LEDEE

UniProtKB: KLLA0A07194p

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Macromolecule #30: Small ribosomal subunit protein uS10

MacromoleculeName: Small ribosomal subunit protein uS10 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 13.337604 KDa
SequenceString:
MSQVEKKSEQ QQEVVIHKIR INLTSTKVKQ LENVSANIIK NAETFKLVKK GPVRLPTKVL KISTRKTPNG EGSKTWDTYE MRIHKRYID LEAPAHIVKR ITQITIEPGV DVEVIIAA

UniProtKB: KLLA0F25542p

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Macromolecule #31: 40S ribosomal protein S25

MacromoleculeName: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 12.002116 KDa
SequenceString:
MPPKQQLSKA AKAAAAMAGG KKSKKKWSKK SHKDKAKHAV VLDQDKFDRI MKEAPTYRYV SVSVLVDRFK LGGSLARVAL RHLENEGII KPVSKHSKQA IYTRATASE

UniProtKB: 40S ribosomal protein S25

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Macromolecule #32: Small ribosomal subunit protein eS28

MacromoleculeName: Small ribosomal subunit protein eS28 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 7.549824 KDa
SequenceString:
MDTKTPVTLA KVIKVLGRTG SRGGVTQVRV EFLEDTTRTI VRNVKGPVRE GDILVLMESE REARRLR

UniProtKB: Small ribosomal subunit protein eS28

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Macromolecule #33: Small ribosomal subunit protein uS14

MacromoleculeName: Small ribosomal subunit protein uS14 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 6.66257 KDa
SequenceString:
MAHENVWYSH PRKFGKGSRQ CRISGSHSGL IRKYGLNIDR QSFREKANDI GFYKYR

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #34: Small ribosomal subunit protein eS31

MacromoleculeName: Small ribosomal subunit protein eS31 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 17.110977 KDa
SequenceString:
MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGGKKR KKKVYTTPK KIRHKHKKVK LAVLNYYKVD DEGKVAKLRK ECPNCGPGIF LANHGDRFYC GKCHSTFATQ K

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

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Macromolecule #35: KLLA0E12277p

MacromoleculeName: KLLA0E12277p / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast)
Molecular weightTheoretical: 35.830945 KDa
SequenceString: MSSSNIMLVL RGTLEGHNGW VTSLSTSAAQ PNLLVSGSRD KTLISWRLTE NEQQFGVPVR SYKGHSHIVQ DVVVSADGNY AVSASWDKT LRLWNLATGN SEARFVGHTG DVLSVAIDAN SSKIISASRD KTIRVWNTVG DCAYVLLGHT DWVTKVRVAP K NLEDGEVD ...String:
MSSSNIMLVL RGTLEGHNGW VTSLSTSAAQ PNLLVSGSRD KTLISWRLTE NEQQFGVPVR SYKGHSHIVQ DVVVSADGNY AVSASWDKT LRLWNLATGN SEARFVGHTG DVLSVAIDAN SSKIISASRD KTIRVWNTVG DCAYVLLGHT DWVTKVRVAP K NLEDGEVD DGRITFVSAG MDKIVRSWSL NEDSYRIEAD FIGHNNYINV VQPSPDGSLA ASAGKDGQIY VWNLKHKSAF MN FDAKDEV FALAFSPSRF WLTAATASGI KIYDLENEVL IDELKPEFAG YTKAQDPHAV SLAWSADGQT LFAGYTDNVI RVW QVMTAN

UniProtKB: KLLA0E12277p

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Macromolecule #36: Eukaryotic translation initiation factor 1A

MacromoleculeName: Eukaryotic translation initiation factor 1A / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 17.462168 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString:
MGKKNTKGGK KGRRGKNDSD GPKRELIYKE EGQEYAQITK MLGNGRVEAS CFDGNKRMAH IRGKLRKKVW MGQGDIILVS LRDFQDDQC DVVHKYNLDE ARTLKNQGEL PENAKINETD NFGFESDEDV NFEFGNADED DEEGEDEELD IDDI

UniProtKB: Eukaryotic translation initiation factor 1A

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Macromolecule #39: Eukaryotic translation initiation factor 2 subunit alpha

MacromoleculeName: Eukaryotic translation initiation factor 2 subunit alpha
type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 34.763652 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MSTSHCRFYE NKYPEIDDIV MVNVQQIAEM GAYVKLLEYD NIEGMILLSE LSRRRIRSIQ KLIRVGKNDV AVVLRVDKEK GYIDLSKRR VSSEDIIKCE EKYQKSKTVH SILRYCAEKF QIPLEELYKT IAWPLSRKFG HAYEAFKLSI IDETVWEGIE P PSKDVLDE ...String:
MSTSHCRFYE NKYPEIDDIV MVNVQQIAEM GAYVKLLEYD NIEGMILLSE LSRRRIRSIQ KLIRVGKNDV AVVLRVDKEK GYIDLSKRR VSSEDIIKCE EKYQKSKTVH SILRYCAEKF QIPLEELYKT IAWPLSRKFG HAYEAFKLSI IDETVWEGIE P PSKDVLDE LKNYISKRLT PQAVKIRADV EVSCFSYEGI DAIKDALKSA EDMSTEQMQV KVKLVAAPLY VLTTQALDKQ KG IEQLESA IEKITEVITK YGGVCNITMP PKAVTATEDA ELQALLESKE LDNRSDSEDD EDESDDE

UniProtKB: Eukaryotic translation initiation factor 2 subunit alpha

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Macromolecule #40: Eukaryotic translation initiation factor 2 subunit gamma

MacromoleculeName: Eukaryotic translation initiation factor 2 subunit gamma
type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO / EC number: protein-synthesizing GTPase
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 57.942699 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MSDLQDQEPS IIINGNLEPV GEPDIVEETE VVAQETQETQ DADKPKKKVA FTGLEEDGET EEEKRKREFE EGGGLPEQPL NPDFSKLNP LSAEIINRQA TINIGTIGHV AHGKSTVVRA ISGVQTVRFK DELERNITIK LGYANAKIYK CQEPTCPEPD C YRSFKSDK ...String:
MSDLQDQEPS IIINGNLEPV GEPDIVEETE VVAQETQETQ DADKPKKKVA FTGLEEDGET EEEKRKREFE EGGGLPEQPL NPDFSKLNP LSAEIINRQA TINIGTIGHV AHGKSTVVRA ISGVQTVRFK DELERNITIK LGYANAKIYK CQEPTCPEPD C YRSFKSDK EISPKCQRPG CPGRYKLVRH VSFVDCPGHD ILMSTMLSGA AVMDAALLLI AGNESCPQPQ TSEHLAAIEI MK LKHVIIL QNKVDLMREE SALEHQKSIL KFIRGTIADG APIVPISAQL KYNIDAVNEF IVKTIPVPPR DFMISPRLIV IRS FDVNKP GAEIEDLKGG VAGGSILNGV FKLGDEIEIR PGIVTKDDKG KIQCKPIFSN IVSLFAEQND LKFAVPGGLI GVGT KVDPT LCRADRLVGQ VVGAKGHLPN IYTDIEINYF LLRRLLGVKT DGQKQAKVRK LEPNEVLMVN IGSTATGARV VAVKA DMAR LQLTSPACTE INEKIALSRR IEKHWRLIGW ATIKKGTTLE PIA

UniProtKB: Eukaryotic translation initiation factor 2 subunit gamma

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Macromolecule #41: Eukaryotic translation initiation factor 2 subunit beta

MacromoleculeName: Eukaryotic translation initiation factor 2 subunit beta
type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 31.631309 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MSSDLAAELG FDPALKKKKK TKKVIPDDFD AAVNGKENGS GDDLFAGLKK KKKKSKSVSA DAEAEKEPTD DIAEALGELS LKKKKKKTK DSSVDAFEKE LAKAGLDNVD AESKEGTPSA NSSIQQEVGL PYSELLSRFF NILRTNNPEL AGDRSGPKFR I PPPVCLRD ...String:
MSSDLAAELG FDPALKKKKK TKKVIPDDFD AAVNGKENGS GDDLFAGLKK KKKKSKSVSA DAEAEKEPTD DIAEALGELS LKKKKKKTK DSSVDAFEKE LAKAGLDNVD AESKEGTPSA NSSIQQEVGL PYSELLSRFF NILRTNNPEL AGDRSGPKFR I PPPVCLRD GKKTIFSNIQ DIAEKLHRSP EHLIQYLFAE LGTSGSVDGQ KRLVIKGKFQ SKQMENVLRR YILEYVTCKT CK SINTELK REQSNRLFFM VCKSCGSTRS VSSIKTGFQA TVGKRRRM

UniProtKB: Eukaryotic translation initiation factor 2 subunit beta

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Macromolecule #42: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 42 / Number of copies: 115 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #43: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 43 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #44: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

MacromoleculeName: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / type: ligand / ID: 44 / Number of copies: 1 / Formula: GCP
Molecular weightTheoretical: 521.208 Da
Chemical component information

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

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Macromolecule #45: METHIONINE

MacromoleculeName: METHIONINE / type: ligand / ID: 45 / Number of copies: 1 / Formula: MET
Molecular weightTheoretical: 149.211 Da
Chemical component information

ChemComp-MET:
METHIONINE

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Macromolecule #46: water

MacromoleculeName: water / type: ligand / ID: 46 / Number of copies: 3 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.1 mg/mL
BufferpH: 6.5
Component:
ConcentrationName
20.0 mMMES
5.0 mMMagnessium acetate
80.0 mMPotassium acetate
10.0 mMAmmonium acetate
2.0 mMDithiothreitol (DTT)
0.001 mMZinc acetate
0.6 mMATP
0.25 mMGDPCP
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
TemperatureMin: 90.0 K / Max: 100.0 K
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Number grids imaged: 8 / Number real images: 11245 / Average exposure time: 1.1 sec. / Average electron dose: 30.0 e/Å2
Details: Images were collected in movie-mode at 30 frames per second
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 59000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

DetailsFEI Falcon III
Particle selectionNumber selected: 1680000 / Details: Selected particles after 2d-classification
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

3jam

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.35 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 66519
Initial angle assignmentType: NOT APPLICABLE / Software - Name: RELION (ver. 1.4)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 3.0)
Final 3D classificationSoftware - Name: RELION (ver. 3.0)

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Atomic model buiding 1

Initial modelPDB ID:

6fyx


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: RECIPROCAL / Protocol: OTHER / Overall B value: 123 / Target criteria: FSC
Output model

PDB-8rw1:
Structure of a yeast 48S-AUC preinitiation complex in closed conformation

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