8RG0

Structure of human eIF3 core from closed 48S translation initiation complex

Functional Information from GO Data

Chain	GOid	namespace	contents
3	0001732	biological_process	formation of cytoplasmic translation initiation complex
3	0002183	biological_process	cytoplasmic translational initiation
3	0003723	molecular_function	RNA binding
3	0003743	molecular_function	translation initiation factor activity
3	0005515	molecular_function	protein binding
3	0005634	cellular_component	nucleus
3	0005737	cellular_component	cytoplasm
3	0005829	cellular_component	cytosol
3	0005852	cellular_component	eukaryotic translation initiation factor 3 complex
3	0006412	biological_process	translation
3	0006413	biological_process	translational initiation
3	0006446	biological_process	regulation of translational initiation
3	0016020	cellular_component	membrane
3	0016282	cellular_component	eukaryotic 43S preinitiation complex
3	0033290	cellular_component	eukaryotic 48S preinitiation complex
3	0043022	molecular_function	ribosome binding
4	0001732	biological_process	formation of cytoplasmic translation initiation complex
4	0002183	biological_process	cytoplasmic translational initiation
4	0003743	molecular_function	translation initiation factor activity
4	0004843	molecular_function	cysteine-type deubiquitinase activity
4	0005515	molecular_function	protein binding
4	0005737	cellular_component	cytoplasm
4	0005829	cellular_component	cytosol
4	0005852	cellular_component	eukaryotic translation initiation factor 3 complex
4	0006412	biological_process	translation
4	0006413	biological_process	translational initiation
4	0006508	biological_process	proteolysis
4	0008233	molecular_function	peptidase activity
4	0008234	molecular_function	cysteine-type peptidase activity
4	0008237	molecular_function	metallopeptidase activity
4	0016020	cellular_component	membrane
4	0016282	cellular_component	eukaryotic 43S preinitiation complex
4	0016787	molecular_function	hydrolase activity
4	0031369	molecular_function	translation initiation factor binding
4	0033290	cellular_component	eukaryotic 48S preinitiation complex
4	0042802	molecular_function	identical protein binding
4	0045202	cellular_component	synapse
4	0071541	cellular_component	eukaryotic translation initiation factor 3 complex, eIF3m
4	0075522	biological_process	IRES-dependent viral translational initiation
4	0101005	molecular_function	deubiquitinase activity
4	0140492	molecular_function	metal-dependent deubiquitinase activity
5	0001650	cellular_component	fibrillar center
5	0001732	biological_process	formation of cytoplasmic translation initiation complex
5	0002183	biological_process	cytoplasmic translational initiation
5	0003723	molecular_function	RNA binding
5	0003743	molecular_function	translation initiation factor activity
5	0005515	molecular_function	protein binding
5	0005654	cellular_component	nucleoplasm
5	0005730	cellular_component	nucleolus
5	0005737	cellular_component	cytoplasm
5	0005829	cellular_component	cytosol
5	0005852	cellular_component	eukaryotic translation initiation factor 3 complex
5	0006412	biological_process	translation
5	0006413	biological_process	translational initiation
5	0016020	cellular_component	membrane
5	0016282	cellular_component	eukaryotic 43S preinitiation complex
5	0032991	cellular_component	protein-containing complex
5	0033290	cellular_component	eukaryotic 48S preinitiation complex
5	0045202	cellular_component	synapse
5	0075525	biological_process	viral translational termination-reinitiation
6	0001732	biological_process	formation of cytoplasmic translation initiation complex
6	0002183	biological_process	cytoplasmic translational initiation
6	0003743	molecular_function	translation initiation factor activity
6	0005515	molecular_function	protein binding
6	0005737	cellular_component	cytoplasm
6	0005829	cellular_component	cytosol
6	0005852	cellular_component	eukaryotic translation initiation factor 3 complex
6	0006412	biological_process	translation
6	0006413	biological_process	translational initiation
6	0016282	cellular_component	eukaryotic 43S preinitiation complex
6	0031369	molecular_function	translation initiation factor binding
6	0032991	cellular_component	protein-containing complex
6	0033290	cellular_component	eukaryotic 48S preinitiation complex
6	0071541	cellular_component	eukaryotic translation initiation factor 3 complex, eIF3m
8	0001732	biological_process	formation of cytoplasmic translation initiation complex
8	0002183	biological_process	cytoplasmic translational initiation
8	0003723	molecular_function	RNA binding
8	0003743	molecular_function	translation initiation factor activity
8	0005515	molecular_function	protein binding
8	0005737	cellular_component	cytoplasm
8	0005829	cellular_component	cytosol
8	0005852	cellular_component	eukaryotic translation initiation factor 3 complex
8	0006412	biological_process	translation
8	0006413	biological_process	translational initiation
8	0006446	biological_process	regulation of translational initiation
8	0008233	molecular_function	peptidase activity
8	0008237	molecular_function	metallopeptidase activity
8	0016020	cellular_component	membrane
8	0016282	cellular_component	eukaryotic 43S preinitiation complex
8	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
8	0033290	cellular_component	eukaryotic 48S preinitiation complex
8	0070062	cellular_component	extracellular exosome
8	0071541	cellular_component	eukaryotic translation initiation factor 3 complex, eIF3m
8	0140492	molecular_function	metal-dependent deubiquitinase activity
H	0000028	biological_process	ribosomal small subunit assembly
H	0002181	biological_process	cytoplasmic translation
H	0003677	molecular_function	DNA binding
H	0003723	molecular_function	RNA binding
H	0003735	molecular_function	structural constituent of ribosome
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005730	cellular_component	nucleolus
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005840	cellular_component	ribosome
H	0006364	biological_process	rRNA processing
H	0006412	biological_process	translation
H	0008270	molecular_function	zinc ion binding
H	0014069	cellular_component	postsynaptic density
H	0022626	cellular_component	cytosolic ribosome
H	0022627	cellular_component	cytosolic small ribosomal subunit
H	0032040	cellular_component	small-subunit processome
H	0042274	biological_process	ribosomal small subunit biogenesis
H	0046872	molecular_function	metal ion binding
H	0098793	cellular_component	presynapse
H	0098978	cellular_component	glutamatergic synapse
H	0098982	cellular_component	GABA-ergic synapse
H	1990904	cellular_component	ribonucleoprotein complex
I	0002181	biological_process	cytoplasmic translation
I	0003723	molecular_function	RNA binding
I	0003729	molecular_function	mRNA binding
I	0003735	molecular_function	structural constituent of ribosome
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005730	cellular_component	nucleolus
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0005840	cellular_component	ribosome
I	0005925	cellular_component	focal adhesion
I	0006412	biological_process	translation
I	0014069	cellular_component	postsynaptic density
I	0016020	cellular_component	membrane
I	0022626	cellular_component	cytosolic ribosome
I	0022627	cellular_component	cytosolic small ribosomal subunit
I	0032040	cellular_component	small-subunit processome
I	0033119	biological_process	negative regulation of RNA splicing
I	0042274	biological_process	ribosomal small subunit biogenesis
I	0048027	molecular_function	mRNA 5'-UTR binding
I	0070062	cellular_component	extracellular exosome
I	0070181	molecular_function	small ribosomal subunit rRNA binding
I	1990904	cellular_component	ribonucleoprotein complex
M	0002181	biological_process	cytoplasmic translation
M	0003723	molecular_function	RNA binding
M	0003735	molecular_function	structural constituent of ribosome
M	0005634	cellular_component	nucleus
M	0005654	cellular_component	nucleoplasm
M	0005730	cellular_component	nucleolus
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0005840	cellular_component	ribosome
M	0005925	cellular_component	focal adhesion
M	0006364	biological_process	rRNA processing
M	0006412	biological_process	translation
M	0006413	biological_process	translational initiation
M	0016020	cellular_component	membrane
M	0022626	cellular_component	cytosolic ribosome
M	0022627	cellular_component	cytosolic small ribosomal subunit
M	0032040	cellular_component	small-subunit processome
M	0034101	biological_process	erythrocyte homeostasis
M	0042274	biological_process	ribosomal small subunit biogenesis
M	1990904	cellular_component	ribonucleoprotein complex
N	0000028	biological_process	ribosomal small subunit assembly
N	0001618	molecular_function	virus receptor activity
N	0002181	biological_process	cytoplasmic translation
N	0003677	molecular_function	DNA binding
N	0003723	molecular_function	RNA binding
N	0003735	molecular_function	structural constituent of ribosome
N	0005055	molecular_function	laminin receptor activity
N	0005515	molecular_function	protein binding
N	0005634	cellular_component	nucleus
N	0005654	cellular_component	nucleoplasm
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0005840	cellular_component	ribosome
N	0005886	cellular_component	plasma membrane
N	0006338	biological_process	chromatin remodeling
N	0006412	biological_process	translation
N	0007155	biological_process	cell adhesion
N	0015935	cellular_component	small ribosomal subunit
N	0016020	cellular_component	membrane
N	0022626	cellular_component	cytosolic ribosome
N	0022627	cellular_component	cytosolic small ribosomal subunit
N	0043022	molecular_function	ribosome binding
N	0043236	molecular_function	laminin binding
N	0046718	biological_process	symbiont entry into host cell
N	0070062	cellular_component	extracellular exosome
N	0140374	biological_process	antiviral innate immune response
N	1990904	cellular_component	ribonucleoprotein complex
O	0002181	biological_process	cytoplasmic translation
O	0003723	molecular_function	RNA binding
O	0003735	molecular_function	structural constituent of ribosome
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0005730	cellular_component	nucleolus
O	0005737	cellular_component	cytoplasm
O	0005783	cellular_component	endoplasmic reticulum
O	0005829	cellular_component	cytosol
O	0005840	cellular_component	ribosome
O	0005925	cellular_component	focal adhesion
O	0006412	biological_process	translation
O	0006413	biological_process	translational initiation
O	0022626	cellular_component	cytosolic ribosome
O	0022627	cellular_component	cytosolic small ribosomal subunit
O	0030154	biological_process	cell differentiation
O	0032040	cellular_component	small-subunit processome
O	0042274	biological_process	ribosomal small subunit biogenesis
O	0043066	biological_process	negative regulation of apoptotic process
O	0045202	cellular_component	synapse
O	0048027	molecular_function	mRNA 5'-UTR binding
O	0070062	cellular_component	extracellular exosome
O	1990904	cellular_component	ribonucleoprotein complex
P	0000028	biological_process	ribosomal small subunit assembly
P	0000122	biological_process	negative regulation of transcription by RNA polymerase II
P	0002181	biological_process	cytoplasmic translation
P	0003723	molecular_function	RNA binding
P	0003735	molecular_function	structural constituent of ribosome
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005730	cellular_component	nucleolus
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0005840	cellular_component	ribosome
P	0005925	cellular_component	focal adhesion
P	0006412	biological_process	translation
P	0014069	cellular_component	postsynaptic density
P	0016020	cellular_component	membrane
P	0022626	cellular_component	cytosolic ribosome
P	0022627	cellular_component	cytosolic small ribosomal subunit
P	0030218	biological_process	erythrocyte differentiation
P	0030490	biological_process	maturation of SSU-rRNA
P	0032040	cellular_component	small-subunit processome
P	0042274	biological_process	ribosomal small subunit biogenesis
P	0045202	cellular_component	synapse
P	0048027	molecular_function	mRNA 5'-UTR binding
P	0070062	cellular_component	extracellular exosome
P	1990904	cellular_component	ribonucleoprotein complex
Q	0002181	biological_process	cytoplasmic translation
Q	0003723	molecular_function	RNA binding
Q	0003729	molecular_function	mRNA binding
Q	0003735	molecular_function	structural constituent of ribosome
Q	0005515	molecular_function	protein binding
Q	0005654	cellular_component	nucleoplasm
Q	0005737	cellular_component	cytoplasm
Q	0005783	cellular_component	endoplasmic reticulum
Q	0005791	cellular_component	rough endoplasmic reticulum
Q	0005829	cellular_component	cytosol
Q	0005840	cellular_component	ribosome
Q	0006412	biological_process	translation
Q	0015935	cellular_component	small ribosomal subunit
Q	0016020	cellular_component	membrane
Q	0022626	cellular_component	cytosolic ribosome
Q	0022627	cellular_component	cytosolic small ribosomal subunit
Q	0033119	biological_process	negative regulation of RNA splicing
Q	0045202	cellular_component	synapse
Q	0045296	molecular_function	cadherin binding
Q	0070062	cellular_component	extracellular exosome
Q	0098556	cellular_component	cytoplasmic side of rough endoplasmic reticulum membrane
Q	1990904	cellular_component	ribonucleoprotein complex
n	0000028	biological_process	ribosomal small subunit assembly
n	0002181	biological_process	cytoplasmic translation
n	0003723	molecular_function	RNA binding
n	0003735	molecular_function	structural constituent of ribosome
n	0005515	molecular_function	protein binding
n	0005634	cellular_component	nucleus
n	0005654	cellular_component	nucleoplasm
n	0005730	cellular_component	nucleolus
n	0005737	cellular_component	cytoplasm
n	0005783	cellular_component	endoplasmic reticulum
n	0005791	cellular_component	rough endoplasmic reticulum
n	0005829	cellular_component	cytosol
n	0005840	cellular_component	ribosome
n	0006364	biological_process	rRNA processing
n	0006412	biological_process	translation
n	0015935	cellular_component	small ribosomal subunit
n	0022626	cellular_component	cytosolic ribosome
n	0022627	cellular_component	cytosolic small ribosomal subunit
n	0030490	biological_process	maturation of SSU-rRNA
n	0032040	cellular_component	small-subunit processome
n	0042254	biological_process	ribosome biogenesis
n	0042274	biological_process	ribosomal small subunit biogenesis
n	0045202	cellular_component	synapse
n	0070062	cellular_component	extracellular exosome
n	0098556	cellular_component	cytoplasmic side of rough endoplasmic reticulum membrane
n	1990904	cellular_component	ribonucleoprotein complex
u	0001732	biological_process	formation of cytoplasmic translation initiation complex
u	0002183	biological_process	cytoplasmic translational initiation
u	0002188	biological_process	translation reinitiation
u	0003723	molecular_function	RNA binding
u	0003729	molecular_function	mRNA binding
u	0003743	molecular_function	translation initiation factor activity
u	0005198	molecular_function	structural molecule activity
u	0005515	molecular_function	protein binding
u	0005654	cellular_component	nucleoplasm
u	0005730	cellular_component	nucleolus
u	0005737	cellular_component	cytoplasm
u	0005829	cellular_component	cytosol
u	0005852	cellular_component	eukaryotic translation initiation factor 3 complex
u	0005874	cellular_component	microtubule
u	0006412	biological_process	translation
u	0006413	biological_process	translational initiation
u	0014069	cellular_component	postsynaptic density
u	0016020	cellular_component	membrane
u	0016282	cellular_component	eukaryotic 43S preinitiation complex
u	0030971	molecular_function	receptor tyrosine kinase binding
u	0032991	cellular_component	protein-containing complex
u	0033290	cellular_component	eukaryotic 48S preinitiation complex
u	0043226	cellular_component	organelle
u	0043614	cellular_component	multi-eIF complex
u	0070373	biological_process	negative regulation of ERK1 and ERK2 cascade
u	0071540	cellular_component	eukaryotic translation initiation factor 3 complex, eIF3e
u	0071541	cellular_component	eukaryotic translation initiation factor 3 complex, eIF3m
u	0075522	biological_process	IRES-dependent viral translational initiation
u	0075525	biological_process	viral translational termination-reinitiation
v	0000184	biological_process	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
v	0000785	cellular_component	chromatin
v	0001732	biological_process	formation of cytoplasmic translation initiation complex
v	0002183	biological_process	cytoplasmic translational initiation
v	0003723	molecular_function	RNA binding
v	0003743	molecular_function	translation initiation factor activity
v	0005515	molecular_function	protein binding
v	0005634	cellular_component	nucleus
v	0005654	cellular_component	nucleoplasm
v	0005737	cellular_component	cytoplasm
v	0005829	cellular_component	cytosol
v	0005852	cellular_component	eukaryotic translation initiation factor 3 complex
v	0006412	biological_process	translation
v	0006413	biological_process	translational initiation
v	0006446	biological_process	regulation of translational initiation
v	0014069	cellular_component	postsynaptic density
v	0016020	cellular_component	membrane
v	0016282	cellular_component	eukaryotic 43S preinitiation complex
v	0016605	cellular_component	PML body
v	0032991	cellular_component	protein-containing complex
v	0033290	cellular_component	eukaryotic 48S preinitiation complex
v	0045296	molecular_function	cadherin binding
v	0045727	biological_process	positive regulation of translation
v	0045947	biological_process	negative regulation of translational initiation
v	0070062	cellular_component	extracellular exosome
v	0071540	cellular_component	eukaryotic translation initiation factor 3 complex, eIF3e
x	0001732	biological_process	formation of cytoplasmic translation initiation complex
x	0002183	biological_process	cytoplasmic translational initiation
x	0002191	biological_process	cap-dependent translational initiation
x	0003723	molecular_function	RNA binding
x	0003743	molecular_function	translation initiation factor activity
x	0005515	molecular_function	protein binding
x	0005737	cellular_component	cytoplasm
x	0005829	cellular_component	cytosol
x	0005852	cellular_component	eukaryotic translation initiation factor 3 complex
x	0006412	biological_process	translation
x	0006413	biological_process	translational initiation
x	0016020	cellular_component	membrane
x	0016282	cellular_component	eukaryotic 43S preinitiation complex
x	0033290	cellular_component	eukaryotic 48S preinitiation complex
x	0045202	cellular_component	synapse
x	0071541	cellular_component	eukaryotic translation initiation factor 3 complex, eIF3m
x	0075522	biological_process	IRES-dependent viral translational initiation
x	0075525	biological_process	viral translational termination-reinitiation
x	0098808	molecular_function	mRNA cap binding
y	0001732	biological_process	formation of cytoplasmic translation initiation complex
y	0002183	biological_process	cytoplasmic translational initiation
y	0003723	molecular_function	RNA binding
y	0003743	molecular_function	translation initiation factor activity
y	0005515	molecular_function	protein binding
y	0005737	cellular_component	cytoplasm
y	0005829	cellular_component	cytosol
y	0005852	cellular_component	eukaryotic translation initiation factor 3 complex
y	0006412	biological_process	translation
y	0006413	biological_process	translational initiation
y	0016282	cellular_component	eukaryotic 43S preinitiation complex
y	0031369	molecular_function	translation initiation factor binding
y	0033290	cellular_component	eukaryotic 48S preinitiation complex
y	0043022	molecular_function	ribosome binding
y	0045727	biological_process	positive regulation of translation

Functional Information from PROSITE/UniProt

site_id	PS00054
Number of Residues	23
Details	RIBOSOMAL_S11 Ribosomal protein S11 signature. LrALarS.GMkIgrieDvTPiPSD

Chain	Residue	Details
P	LEU116-ASP138

---

site_id	PS00362
Number of Residues	31
Details	RIBOSOMAL_S15 Ribosomal protein S15 signature. VrkHLernRkDkdAkfrLilIesrihrLarY

Chain	Residue	Details
I	VAL98-TYR128

---

site_id	PS00712
Number of Residues	16
Details	RIBOSOMAL_S17E Ribosomal protein S17e signature. IpSKkLRNkIAGYvTH

Chain	Residue	Details
M	ILE41-HIS56

---

site_id	PS00733
Number of Residues	8
Details	RIBOSOMAL_S26E Ribosomal protein S26e signature. YCVSCAIH

Chain	Residue	Details
Q	TYR73-HIS80

---

site_id	PS00961
Number of Residues	9
Details	RIBOSOMAL_S28E Ribosomal protein S28e signature. ESEREARrL

Chain	Residue	Details
n	GLU60-LEU68

---

site_id	PS00962
Number of Residues	12
Details	RIBOSOMAL_S2_1 Ribosomal protein S2 signature 1. VlKFLAAGTHLG

Chain	Residue	Details
N	VAL15-GLY26

---

site_id	PS00963
Number of Residues	25
Details	RIBOSOMAL_S2_2 Ribosomal protein S2 signature 2. PrlLVVtDpraDhqpLtEasyvNLP

Chain	Residue	Details
N	PRO119-PRO143

---

site_id	PS01168
Number of Residues	33
Details	RIBOSOMAL_S27E Ribosomal protein S27e signature. KCpgCykittvFShAqtvvlCvgCStvLcqPtG

Chain	Residue	Details
H	LYS36-GLY68

---

site_id	PS01191
Number of Residues	13
Details	RIBOSOMAL_S3AE Ribosomal protein S3Ae signature. LkGRVfEvSLaDL

Chain	Residue	Details
O	LEU62-LEU74

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P62301

Chain	Residue	Details
I	LYS27
y	SER11
y	SER13
y	SER15
y	SER16
y	SER18
y	SER39
y	SER166
y	SER909

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
I	SER30
H	CYS40
H	CYS56
H	CYS59

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:P62301

Chain	Residue	Details
I	LYS34
5	LYS549
y	SER182
u	SER1028
u	SER1188
u	SER1262

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:15592455

Chain	Residue	Details
I	TYR38
u	SER1198
u	SER1336
P	LYS106

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:36638793

Chain	Residue	Details
I	LYS27

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
u	SER1364
I	LYS43

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:16964243, ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:24275569

Chain	Residue	Details
O	SER263

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744|PubMed:28112733

Chain	Residue	Details
O	LYS34

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:28112733

Chain	Residue	Details
O	LYS249

---