8RCH
CryoEM structure of mTORC1 with a paediatric kidney cancer-associated 1455-EWED-1458 duplication in mTOR, overall refinement
Functional Information from PROSITE/UniProt
| site_id | PS00678 |
| Number of Residues | 15 |
| Details | WD_REPEATS_1 Trp-Asp (WD) repeats signature. MYTGgeDcTARIWDL |
| Chain | Residue | Details |
| D | MET100-LEU114 |
| site_id | PS00915 |
| Number of Residues | 15 |
| Details | PI3_4_KINASE_1 Phosphatidylinositol 3- and 4-kinases signature 1. LKgh.EDLRQDervmQ |
| Chain | Residue | Details |
| A | LEU2186-GLN2200 |
| site_id | PS00916 |
| Number of Residues | 21 |
| Details | PI3_4_KINASE_2 Phosphatidylinositol 3- and 4-kinases signature 2. SlAvmsMvgYILgLgDRHpsN |
| Chain | Residue | Details |
| A | SER2323-ASN2343 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | MOD_RES: N-acetylserine => ECO:0000269|PubMed:12665801, ECO:0007744|PubMed:22814378 |
| Chain | Residue | Details |
| Z | SER2 | |
| A | MET2345 | |
| A | ILE2356 | |
| B | SER2165 | |
| B | GLN2167 | |
| B | LEU2185 | |
| B | GLU2190 | |
| B | TYR2225 | |
| B | GLY2238 | |
| B | TRP2239 | |
| B | VAL2240 | |
| X | SER2 | |
| B | THR2245 | |
| B | MET2345 | |
| B | ILE2356 | |
| W | SER836 | |
| W | SER982 | |
| Y | SER44 | |
| Y | SER122 | |
| Y | SER836 | |
| Y | SER982 | |
| A | THR2245 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphothreonine; by MTOR => ECO:0000269|PubMed:12747827, ECO:0000269|PubMed:22578813, ECO:0000269|PubMed:29236692, ECO:0000269|PubMed:29750193, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| Z | THR37 | |
| X | THR37 | |
| A | ASP2357 | |
| B | LYS2187 | |
| B | ASN2343 | |
| B | ASP2357 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| Z | THR41 | |
| X | THR41 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q60876 |
| Chain | Residue | Details |
| Z | SER44 | |
| Z | SER96 | |
| X | SER44 | |
| X | SER96 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphothreonine; by MTOR => ECO:0000269|PubMed:12747827, ECO:0000269|PubMed:22578813, ECO:0000269|PubMed:29236692, ECO:0000269|PubMed:29750193, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332 |
| Chain | Residue | Details |
| Z | THR46 | |
| X | THR46 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18220336, ECO:0007744|PubMed:18669648 |
| Chain | Residue | Details |
| Z | THR50 | |
| X | THR50 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18669648 |
| Chain | Residue | Details |
| Z | TYR54 | |
| X | TYR54 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine; by DYRK2, MAPK1, MAPK3 and MTOR => ECO:0000269|PubMed:12588975, ECO:0000269|PubMed:12747827, ECO:0000269|PubMed:22578813, ECO:0000269|PubMed:25702871, ECO:0000269|PubMed:29750193, ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| Z | SER65 | |
| X | SER65 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphothreonine; by MTOR => ECO:0000269|PubMed:12747827, ECO:0000269|PubMed:22578813, ECO:0000269|PubMed:25702871, ECO:0000269|PubMed:29750193, ECO:0007744|PubMed:18220336, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| Z | THR70 | |
| X | THR70 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| Z | THR77 | |
| X | THR77 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648 |
| Chain | Residue | Details |
| Z | SER83 | |
| X | SER83 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine; by DYRK2 => ECO:0000269|PubMed:12588975, ECO:0007744|PubMed:18220336, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| Z | SER101 | |
| X | SER101 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:12588975, ECO:0007744|PubMed:17525332, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| Z | SER112 | |
| X | SER112 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:22578813 |
| Chain | Residue | Details |
| Z | LYS57 | |
| Y | SER877 | |
| X | LYS57 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 2 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:30197302, ECO:0000269|PubMed:32561715 |
| Chain | Residue | Details |
| W | LYS1097 | |
| Y | LYS1097 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 2 |
| Details | CARBOHYD: O-linked (GlcNAc) threonine => ECO:0000269|PubMed:37541260 |
| Chain | Residue | Details |
| W | THR700 | |
| Y | THR700 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 6 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:35927303 |
| Chain | Residue | Details |
| W | LYS932 | |
| W | LYS948 | |
| Y | LYS932 | |
| Y | LYS948 |
