8QYS

Human preholo proteasome 20S core particle

Functional Information from GO Data

Chain	GOid	namespace	contents
a	0005515	molecular_function	protein binding
a	0005634	cellular_component	nucleus
a	0005829	cellular_component	cytosol
a	0007094	biological_process	mitotic spindle assembly checkpoint signaling
a	0043066	biological_process	negative regulation of apoptotic process
a	0043248	biological_process	proteasome assembly
a	0051131	biological_process	chaperone-mediated protein complex assembly
a	0051726	biological_process	regulation of cell cycle
a	0060090	molecular_function	molecular adaptor activity
a	0101031	cellular_component	protein folding chaperone complex
A	0000502	cellular_component	proteasome complex
A	0000932	cellular_component	P-body
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005839	cellular_component	proteasome core complex
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0009615	biological_process	response to virus
A	0019773	cellular_component	proteasome core complex, alpha-subunit complex
A	0034774	cellular_component	secretory granule lumen
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0051603	biological_process	proteolysis involved in protein catabolic process
A	0070062	cellular_component	extracellular exosome
A	1904813	cellular_component	ficolin-1-rich granule lumen
b	0000502	cellular_component	proteasome complex
b	0004175	molecular_function	endopeptidase activity
b	0004298	molecular_function	threonine-type endopeptidase activity
b	0005515	molecular_function	protein binding
b	0005576	cellular_component	extracellular region
b	0005634	cellular_component	nucleus
b	0005654	cellular_component	nucleoplasm
b	0005737	cellular_component	cytoplasm
b	0005829	cellular_component	cytosol
b	0005839	cellular_component	proteasome core complex
b	0006508	biological_process	proteolysis
b	0016604	cellular_component	nuclear body
b	0019774	cellular_component	proteasome core complex, beta-subunit complex
b	0034774	cellular_component	secretory granule lumen
b	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
b	0051603	biological_process	proteolysis involved in protein catabolic process
b	1904813	cellular_component	ficolin-1-rich granule lumen
B	0000502	cellular_component	proteasome complex
B	0000932	cellular_component	P-body
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005839	cellular_component	proteasome core complex
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0019773	cellular_component	proteasome core complex, alpha-subunit complex
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0043231	cellular_component	intracellular membrane-bounded organelle
B	0051603	biological_process	proteolysis involved in protein catabolic process
B	0070062	cellular_component	extracellular exosome
c	0000502	cellular_component	proteasome complex
c	0005515	molecular_function	protein binding
c	0005634	cellular_component	nucleus
c	0005654	cellular_component	nucleoplasm
c	0005737	cellular_component	cytoplasm
c	0005829	cellular_component	cytosol
c	0005839	cellular_component	proteasome core complex
c	0019774	cellular_component	proteasome core complex, beta-subunit complex
c	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
c	0051603	biological_process	proteolysis involved in protein catabolic process
c	0070062	cellular_component	extracellular exosome
C	0000502	cellular_component	proteasome complex
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005839	cellular_component	proteasome core complex
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0019773	cellular_component	proteasome core complex, alpha-subunit complex
C	0042802	molecular_function	identical protein binding
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0051603	biological_process	proteolysis involved in protein catabolic process
C	0070062	cellular_component	extracellular exosome
C	0098794	cellular_component	postsynapse
d	0000502	cellular_component	proteasome complex
d	0005515	molecular_function	protein binding
d	0005634	cellular_component	nucleus
d	0005654	cellular_component	nucleoplasm
d	0005737	cellular_component	cytoplasm
d	0005829	cellular_component	cytosol
d	0005839	cellular_component	proteasome core complex
d	0010243	biological_process	response to organonitrogen compound
d	0010498	biological_process	proteasomal protein catabolic process
d	0014070	biological_process	response to organic cyclic compound
d	0016020	cellular_component	membrane
d	0019774	cellular_component	proteasome core complex, beta-subunit complex
d	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
d	0051603	biological_process	proteolysis involved in protein catabolic process
d	0070062	cellular_component	extracellular exosome
D	0000502	cellular_component	proteasome complex
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005839	cellular_component	proteasome core complex
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0019773	cellular_component	proteasome core complex, alpha-subunit complex
D	0034774	cellular_component	secretory granule lumen
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0051603	biological_process	proteolysis involved in protein catabolic process
D	0070062	cellular_component	extracellular exosome
D	1904813	cellular_component	ficolin-1-rich granule lumen
e	0004298	molecular_function	threonine-type endopeptidase activity
e	0005839	cellular_component	proteasome core complex
e	0051603	biological_process	proteolysis involved in protein catabolic process
E	0000502	cellular_component	proteasome complex
E	0001530	molecular_function	lipopolysaccharide binding
E	0002376	biological_process	immune system process
E	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005813	cellular_component	centrosome
E	0005829	cellular_component	cytosol
E	0005839	cellular_component	proteasome core complex
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0019773	cellular_component	proteasome core complex, alpha-subunit complex
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0051603	biological_process	proteolysis involved in protein catabolic process
E	0070062	cellular_component	extracellular exosome
f	0005839	cellular_component	proteasome core complex
f	0051603	biological_process	proteolysis involved in protein catabolic process
F	0000502	cellular_component	proteasome complex
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005839	cellular_component	proteasome core complex
F	0006511	biological_process	ubiquitin-dependent protein catabolic process
F	0019773	cellular_component	proteasome core complex, alpha-subunit complex
F	0031625	molecular_function	ubiquitin protein ligase binding
F	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
F	0045202	cellular_component	synapse
F	0051603	biological_process	proteolysis involved in protein catabolic process
F	0052548	biological_process	regulation of endopeptidase activity
F	0070062	cellular_component	extracellular exosome
g	0005839	cellular_component	proteasome core complex
g	0051603	biological_process	proteolysis involved in protein catabolic process
G	0000502	cellular_component	proteasome complex
G	0000932	cellular_component	P-body
G	0003723	molecular_function	RNA binding
G	0004175	molecular_function	endopeptidase activity
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005839	cellular_component	proteasome core complex
G	0005840	cellular_component	ribosome
G	0006511	biological_process	ubiquitin-dependent protein catabolic process
G	0016363	cellular_component	nuclear matrix
G	0019773	cellular_component	proteasome core complex, alpha-subunit complex
G	0030016	cellular_component	myofibril
G	0030017	cellular_component	sarcomere
G	0035639	molecular_function	purine ribonucleoside triphosphate binding
G	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
G	0050727	biological_process	regulation of inflammatory response
G	0051059	molecular_function	NF-kappaB binding
G	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
G	0051603	biological_process	proteolysis involved in protein catabolic process
G	0070062	cellular_component	extracellular exosome
h	0004298	molecular_function	threonine-type endopeptidase activity
h	0005839	cellular_component	proteasome core complex
h	0051603	biological_process	proteolysis involved in protein catabolic process
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005783	cellular_component	endoplasmic reticulum
H	0005829	cellular_component	cytosol
H	0016020	cellular_component	membrane
H	0016607	cellular_component	nuclear speck
H	0043248	biological_process	proteasome assembly
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005737	cellular_component	cytoplasm
I	0005783	cellular_component	endoplasmic reticulum
I	0005794	cellular_component	Golgi apparatus
I	0005829	cellular_component	cytosol
I	0021930	biological_process	cerebellar granule cell precursor proliferation
I	0043248	biological_process	proteasome assembly
I	0051131	biological_process	chaperone-mediated protein complex assembly
I	0060090	molecular_function	molecular adaptor activity
I	0070628	molecular_function	proteasome binding
I	0080129	biological_process	proteasome core complex assembly
I	0101031	cellular_component	protein folding chaperone complex
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005829	cellular_component	cytosol
J	0007094	biological_process	mitotic spindle assembly checkpoint signaling
J	0043066	biological_process	negative regulation of apoptotic process
J	0043248	biological_process	proteasome assembly
J	0051131	biological_process	chaperone-mediated protein complex assembly
J	0051726	biological_process	regulation of cell cycle
J	0060090	molecular_function	molecular adaptor activity
J	0101031	cellular_component	protein folding chaperone complex
K	0000502	cellular_component	proteasome complex
K	0004175	molecular_function	endopeptidase activity
K	0004298	molecular_function	threonine-type endopeptidase activity
K	0005515	molecular_function	protein binding
K	0005576	cellular_component	extracellular region
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0005839	cellular_component	proteasome core complex
K	0006508	biological_process	proteolysis
K	0016604	cellular_component	nuclear body
K	0019774	cellular_component	proteasome core complex, beta-subunit complex
K	0034774	cellular_component	secretory granule lumen
K	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
K	0051603	biological_process	proteolysis involved in protein catabolic process
K	1904813	cellular_component	ficolin-1-rich granule lumen
L	0000502	cellular_component	proteasome complex
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0005839	cellular_component	proteasome core complex
L	0019774	cellular_component	proteasome core complex, beta-subunit complex
L	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
L	0051603	biological_process	proteolysis involved in protein catabolic process
L	0070062	cellular_component	extracellular exosome
M	0000502	cellular_component	proteasome complex
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005654	cellular_component	nucleoplasm
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0005839	cellular_component	proteasome core complex
M	0010243	biological_process	response to organonitrogen compound
M	0010498	biological_process	proteasomal protein catabolic process
M	0014070	biological_process	response to organic cyclic compound
M	0016020	cellular_component	membrane
M	0019774	cellular_component	proteasome core complex, beta-subunit complex
M	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
M	0051603	biological_process	proteolysis involved in protein catabolic process
M	0070062	cellular_component	extracellular exosome
N	0004298	molecular_function	threonine-type endopeptidase activity
N	0005839	cellular_component	proteasome core complex
N	0051603	biological_process	proteolysis involved in protein catabolic process
O	0005839	cellular_component	proteasome core complex
O	0051603	biological_process	proteolysis involved in protein catabolic process
P	0005839	cellular_component	proteasome core complex
P	0051603	biological_process	proteolysis involved in protein catabolic process
Q	0004298	molecular_function	threonine-type endopeptidase activity
Q	0005839	cellular_component	proteasome core complex
Q	0051603	biological_process	proteolysis involved in protein catabolic process
R	0000502	cellular_component	proteasome complex
R	0000932	cellular_component	P-body
R	0005515	molecular_function	protein binding
R	0005576	cellular_component	extracellular region
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0005839	cellular_component	proteasome core complex
R	0006511	biological_process	ubiquitin-dependent protein catabolic process
R	0009615	biological_process	response to virus
R	0019773	cellular_component	proteasome core complex, alpha-subunit complex
R	0034774	cellular_component	secretory granule lumen
R	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
R	0051603	biological_process	proteolysis involved in protein catabolic process
R	0070062	cellular_component	extracellular exosome
R	1904813	cellular_component	ficolin-1-rich granule lumen
S	0000502	cellular_component	proteasome complex
S	0000932	cellular_component	P-body
S	0005515	molecular_function	protein binding
S	0005634	cellular_component	nucleus
S	0005654	cellular_component	nucleoplasm
S	0005737	cellular_component	cytoplasm
S	0005829	cellular_component	cytosol
S	0005839	cellular_component	proteasome core complex
S	0006511	biological_process	ubiquitin-dependent protein catabolic process
S	0019773	cellular_component	proteasome core complex, alpha-subunit complex
S	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
S	0043231	cellular_component	intracellular membrane-bounded organelle
S	0051603	biological_process	proteolysis involved in protein catabolic process
S	0070062	cellular_component	extracellular exosome
T	0000502	cellular_component	proteasome complex
T	0005515	molecular_function	protein binding
T	0005634	cellular_component	nucleus
T	0005654	cellular_component	nucleoplasm
T	0005737	cellular_component	cytoplasm
T	0005829	cellular_component	cytosol
T	0005839	cellular_component	proteasome core complex
T	0006511	biological_process	ubiquitin-dependent protein catabolic process
T	0019773	cellular_component	proteasome core complex, alpha-subunit complex
T	0042802	molecular_function	identical protein binding
T	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
T	0051603	biological_process	proteolysis involved in protein catabolic process
T	0070062	cellular_component	extracellular exosome
T	0098794	cellular_component	postsynapse
U	0000502	cellular_component	proteasome complex
U	0005515	molecular_function	protein binding
U	0005576	cellular_component	extracellular region
U	0005634	cellular_component	nucleus
U	0005654	cellular_component	nucleoplasm
U	0005737	cellular_component	cytoplasm
U	0005829	cellular_component	cytosol
U	0005839	cellular_component	proteasome core complex
U	0006511	biological_process	ubiquitin-dependent protein catabolic process
U	0019773	cellular_component	proteasome core complex, alpha-subunit complex
U	0034774	cellular_component	secretory granule lumen
U	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
U	0051603	biological_process	proteolysis involved in protein catabolic process
U	0070062	cellular_component	extracellular exosome
U	1904813	cellular_component	ficolin-1-rich granule lumen
V	0000502	cellular_component	proteasome complex
V	0001530	molecular_function	lipopolysaccharide binding
V	0002376	biological_process	immune system process
V	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
V	0005515	molecular_function	protein binding
V	0005634	cellular_component	nucleus
V	0005654	cellular_component	nucleoplasm
V	0005737	cellular_component	cytoplasm
V	0005813	cellular_component	centrosome
V	0005829	cellular_component	cytosol
V	0005839	cellular_component	proteasome core complex
V	0006511	biological_process	ubiquitin-dependent protein catabolic process
V	0019773	cellular_component	proteasome core complex, alpha-subunit complex
V	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
V	0051603	biological_process	proteolysis involved in protein catabolic process
V	0070062	cellular_component	extracellular exosome
W	0000502	cellular_component	proteasome complex
W	0005515	molecular_function	protein binding
W	0005634	cellular_component	nucleus
W	0005654	cellular_component	nucleoplasm
W	0005737	cellular_component	cytoplasm
W	0005829	cellular_component	cytosol
W	0005839	cellular_component	proteasome core complex
W	0006511	biological_process	ubiquitin-dependent protein catabolic process
W	0019773	cellular_component	proteasome core complex, alpha-subunit complex
W	0031625	molecular_function	ubiquitin protein ligase binding
W	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
W	0045202	cellular_component	synapse
W	0051603	biological_process	proteolysis involved in protein catabolic process
W	0052548	biological_process	regulation of endopeptidase activity
W	0070062	cellular_component	extracellular exosome
X	0000502	cellular_component	proteasome complex
X	0000932	cellular_component	P-body
X	0003723	molecular_function	RNA binding
X	0004175	molecular_function	endopeptidase activity
X	0005515	molecular_function	protein binding
X	0005634	cellular_component	nucleus
X	0005654	cellular_component	nucleoplasm
X	0005737	cellular_component	cytoplasm
X	0005829	cellular_component	cytosol
X	0005839	cellular_component	proteasome core complex
X	0005840	cellular_component	ribosome
X	0006511	biological_process	ubiquitin-dependent protein catabolic process
X	0016363	cellular_component	nuclear matrix
X	0019773	cellular_component	proteasome core complex, alpha-subunit complex
X	0030016	cellular_component	myofibril
X	0030017	cellular_component	sarcomere
X	0035639	molecular_function	purine ribonucleoside triphosphate binding
X	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
X	0050727	biological_process	regulation of inflammatory response
X	0051059	molecular_function	NF-kappaB binding
X	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
X	0051603	biological_process	proteolysis involved in protein catabolic process
X	0070062	cellular_component	extracellular exosome
Y	0005515	molecular_function	protein binding
Y	0005634	cellular_component	nucleus
Y	0005737	cellular_component	cytoplasm
Y	0005783	cellular_component	endoplasmic reticulum
Y	0005829	cellular_component	cytosol
Y	0016020	cellular_component	membrane
Y	0016607	cellular_component	nuclear speck
Y	0043248	biological_process	proteasome assembly
Z	0005515	molecular_function	protein binding
Z	0005634	cellular_component	nucleus
Z	0005654	cellular_component	nucleoplasm
Z	0005737	cellular_component	cytoplasm
Z	0005783	cellular_component	endoplasmic reticulum
Z	0005794	cellular_component	Golgi apparatus
Z	0005829	cellular_component	cytosol
Z	0021930	biological_process	cerebellar granule cell precursor proliferation
Z	0043248	biological_process	proteasome assembly
Z	0051131	biological_process	chaperone-mediated protein complex assembly
Z	0060090	molecular_function	molecular adaptor activity
Z	0070628	molecular_function	proteasome binding
Z	0080129	biological_process	proteasome core complex assembly
Z	0101031	cellular_component	protein folding chaperone complex

Functional Information from PROSITE/UniProt

site_id	PS00388
Number of Residues	23
Details	PROTEASOME_ALPHA_1 Proteasome alpha-type subunits signature. YdraiTvFSPdGHlfQVEYAqeA

Chain	Residue	Details
C	TYR3-ALA25
E	TYR6-ALA28
D	TYR8-ALA30
F	TYR8-ALA30
G	PHE9-ALA31
A	TYR6-ALA28
B	TYR5-ALA27

---

site_id	PS00854
Number of Residues	48
Details	PROTEASOME_BETA_1 Proteasome beta-type subunits signature. LAIaGedFAIVASDtrlsegfsihtrdsp.KcykltdktvigcsGfhGD

Chain	Residue	Details
f	LEU41-ASP88
e	LEU63-ASP110
g	LEU57-ASP104
h	MET38-ASP85
b	ALA47-ASP94
B	LEU35-ASP82
c	MET12-ASP59
d	ILE5-ASP52

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: N-acetylalanine => ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378

Chain	Residue	Details
I	ALA2
Z	ALA2
Y	LYS39
X	SER17
X	SER63
X	SER64

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
I	THR18
I	THR54
Z	THR18
Z	THR54

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
I	SER180
Z	SER180
S	LYS127
S	LYS176
R	SER14
R	SER16

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
I	LYS264
Z	LYS264
f	SER58
f	SER209

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Chain	Residue	Details
E	LYS115
U	SER63
E	LYS208
V	LYS115
V	LYS208

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	CARBOHYD: O-linked (GlcNAc) serine => ECO:0000250

Chain	Residue	Details
D	SER198
U	SER198

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P17220

Chain	Residue	Details
A	TYR121
R	TYR121

---