8PMQ

Catalytic module of yeast GID E3 ligase bound to multiphosphorylated Ubc8~ubiquitin

Functional Information from GO Data

Chain	GOid	namespace	contents
2	0004842	molecular_function	ubiquitin-protein transferase activity
2	0005515	molecular_function	protein binding
2	0005634	cellular_component	nucleus
2	0005737	cellular_component	cytoplasm
2	0005777	cellular_component	peroxisome
2	0005829	cellular_component	cytosol
2	0006511	biological_process	ubiquitin-dependent protein catabolic process
2	0008270	molecular_function	zinc ion binding
2	0016567	biological_process	protein ubiquitination
2	0016740	molecular_function	transferase activity
2	0034657	cellular_component	GID complex
2	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
2	0045721	biological_process	negative regulation of gluconeogenesis
2	0046872	molecular_function	metal ion binding
2	0061630	molecular_function	ubiquitin protein ligase activity
9	0004842	molecular_function	ubiquitin-protein transferase activity
9	0005515	molecular_function	protein binding
9	0005634	cellular_component	nucleus
9	0005737	cellular_component	cytoplasm
9	0016567	biological_process	protein ubiquitination
9	0016740	molecular_function	transferase activity
9	0034657	cellular_component	GID complex
9	0043066	biological_process	negative regulation of apoptotic process
9	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
9	0045721	biological_process	negative regulation of gluconeogenesis
9	0046872	molecular_function	metal ion binding
9	0061630	molecular_function	ubiquitin protein ligase activity
E	0000209	biological_process	protein polyubiquitination
E	0004842	molecular_function	ubiquitin-protein transferase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0016567	biological_process	protein ubiquitination
E	0016740	molecular_function	transferase activity
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0045721	biological_process	negative regulation of gluconeogenesis
E	0061631	molecular_function	ubiquitin conjugating enzyme activity

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
U	LYS27-ASP52

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	SITE: Interacts with activating enzyme

Chain	Residue	Details
U	ARG54
U	ARG72

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site_id	SWS_FT_FI2
Number of Residues	1
Details	SITE: Essential for function

Chain	Residue	Details
U	HIS68

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site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291

Chain	Residue	Details
U	SER65

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site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457

Chain	Residue	Details
U	THR66

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site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: ADP-ribosylglycine => ECO:0000269|PubMed:28525742

Chain	Residue	Details
U	GLY76

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site_id	SWS_FT_FI6
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603

Chain	Residue	Details
U	LYS6

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site_id	SWS_FT_FI7
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Chain	Residue	Details
U	GLY76

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site_id	SWS_FT_FI8
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144

Chain	Residue	Details
U	LYS11
U	LYS48

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site_id	SWS_FT_FI9
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860

Chain	Residue	Details
U	LYS27

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site_id	SWS_FT_FI10
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127

Chain	Residue	Details
U	LYS29

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site_id	SWS_FT_FI11
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577

Chain	Residue	Details
U	LYS33

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site_id	SWS_FT_FI12
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106

Chain	Residue	Details
U	LYS63

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