8PJN
Catalytic module of human CTLH E3 ligase bound to multiphosphorylated UBE2H~ubiquitin
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| 2 | 0000209 | biological_process | protein polyubiquitination |
| 2 | 0004842 | molecular_function | ubiquitin-protein transferase activity |
| 2 | 0005515 | molecular_function | protein binding |
| 2 | 0005829 | cellular_component | cytosol |
| 2 | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| 2 | 0016567 | biological_process | protein ubiquitination |
| 2 | 0061631 | molecular_function | ubiquitin conjugating enzyme activity |
| 2 | 0070936 | biological_process | protein K48-linked ubiquitination |
| 2 | 0070979 | biological_process | protein K11-linked ubiquitination |
| b | 0000151 | cellular_component | ubiquitin ligase complex |
| b | 0000209 | biological_process | protein polyubiquitination |
| b | 0004842 | molecular_function | ubiquitin-protein transferase activity |
| b | 0005515 | molecular_function | protein binding |
| b | 0005634 | cellular_component | nucleus |
| b | 0005654 | cellular_component | nucleoplasm |
| b | 0005737 | cellular_component | cytoplasm |
| b | 0005829 | cellular_component | cytosol |
| b | 0034657 | cellular_component | GID complex |
| b | 0043161 | biological_process | proteasome-mediated ubiquitin-dependent protein catabolic process |
| b | 0061630 | molecular_function | ubiquitin protein ligase activity |
| i | 0000151 | cellular_component | ubiquitin ligase complex |
| i | 0003779 | molecular_function | actin binding |
| i | 0004842 | molecular_function | ubiquitin-protein transferase activity |
| i | 0005634 | cellular_component | nucleus |
| i | 0005654 | cellular_component | nucleoplasm |
| i | 0005737 | cellular_component | cytoplasm |
| i | 0005819 | cellular_component | spindle |
| i | 0005826 | cellular_component | actomyosin contractile ring |
| i | 0005829 | cellular_component | cytosol |
| i | 0005856 | cellular_component | cytoskeleton |
| i | 0005886 | cellular_component | plasma membrane |
| i | 0007155 | biological_process | cell adhesion |
| i | 0007346 | biological_process | regulation of mitotic cell cycle |
| i | 0015629 | cellular_component | actin cytoskeleton |
| i | 0016363 | cellular_component | nuclear matrix |
| i | 0033033 | biological_process | negative regulation of myeloid cell apoptotic process |
| i | 0034657 | cellular_component | GID complex |
| i | 0043161 | biological_process | proteasome-mediated ubiquitin-dependent protein catabolic process |
| i | 0061630 | molecular_function | ubiquitin protein ligase activity |
Functional Information from PROSITE/UniProt
| site_id | PS00299 |
| Number of Residues | 26 |
| Details | UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD |
| Chain | Residue | Details |
| u | LYS27-ASP52 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 64 |
| Details | Domain: {"description":"LisH","evidences":[{"source":"PROSITE-ProRule","id":"PRU00126","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 108 |
| Details | Zinc finger: {"description":"RING-Gid-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU01215","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Glycyl thioester intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU00388","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10133","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P62257","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 57 |
| Details | Domain: {"description":"CTLH","evidences":[{"source":"PROSITE-ProRule","id":"PRU00058","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | Site: {"description":"Essential for ubiquitin ligase activity","evidences":[{"source":"UniProtKB","id":"P40492","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 75 |
| Details | Domain: {"description":"Ubiquitin-like 9","evidences":[{"source":"PROSITE-ProRule","id":"PRU00214","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
