8PJN
Catalytic module of human CTLH E3 ligase bound to multiphosphorylated UBE2H~ubiquitin
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| 2 | 0000209 | biological_process | protein polyubiquitination |
| 2 | 0004842 | molecular_function | ubiquitin-protein transferase activity |
| 2 | 0005515 | molecular_function | protein binding |
| 2 | 0005524 | molecular_function | ATP binding |
| 2 | 0005634 | cellular_component | nucleus |
| 2 | 0005829 | cellular_component | cytosol |
| 2 | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| 2 | 0016567 | biological_process | protein ubiquitination |
| 2 | 0016740 | molecular_function | transferase activity |
| 2 | 0019787 | molecular_function | ubiquitin-like protein transferase activity |
| 2 | 0043161 | biological_process | proteasome-mediated ubiquitin-dependent protein catabolic process |
| 2 | 0061631 | molecular_function | ubiquitin conjugating enzyme activity |
| 2 | 0070936 | biological_process | protein K48-linked ubiquitination |
| 2 | 0070979 | biological_process | protein K11-linked ubiquitination |
| b | 0000151 | cellular_component | ubiquitin ligase complex |
| b | 0000209 | biological_process | protein polyubiquitination |
| b | 0004842 | molecular_function | ubiquitin-protein transferase activity |
| b | 0005515 | molecular_function | protein binding |
| b | 0005634 | cellular_component | nucleus |
| b | 0005654 | cellular_component | nucleoplasm |
| b | 0005737 | cellular_component | cytoplasm |
| b | 0005829 | cellular_component | cytosol |
| b | 0016740 | molecular_function | transferase activity |
| b | 0034657 | cellular_component | GID complex |
| b | 0043161 | biological_process | proteasome-mediated ubiquitin-dependent protein catabolic process |
| b | 0046872 | molecular_function | metal ion binding |
| b | 0061630 | molecular_function | ubiquitin protein ligase activity |
| i | 0000151 | cellular_component | ubiquitin ligase complex |
| i | 0003779 | molecular_function | actin binding |
| i | 0004842 | molecular_function | ubiquitin-protein transferase activity |
| i | 0005634 | cellular_component | nucleus |
| i | 0005654 | cellular_component | nucleoplasm |
| i | 0005737 | cellular_component | cytoplasm |
| i | 0005819 | cellular_component | spindle |
| i | 0005826 | cellular_component | actomyosin contractile ring |
| i | 0005829 | cellular_component | cytosol |
| i | 0005856 | cellular_component | cytoskeleton |
| i | 0005886 | cellular_component | plasma membrane |
| i | 0007010 | biological_process | cytoskeleton organization |
| i | 0007155 | biological_process | cell adhesion |
| i | 0007346 | biological_process | regulation of mitotic cell cycle |
| i | 0015629 | cellular_component | actin cytoskeleton |
| i | 0016363 | cellular_component | nuclear matrix |
| i | 0016740 | molecular_function | transferase activity |
| i | 0033033 | biological_process | negative regulation of myeloid cell apoptotic process |
| i | 0034657 | cellular_component | GID complex |
| i | 0043161 | biological_process | proteasome-mediated ubiquitin-dependent protein catabolic process |
| i | 0043249 | biological_process | erythrocyte maturation |
| i | 0046872 | molecular_function | metal ion binding |
| i | 0048821 | biological_process | erythrocyte development |
| i | 0048822 | biological_process | enucleate erythrocyte development |
| i | 0051301 | biological_process | cell division |
| i | 0061630 | molecular_function | ubiquitin protein ligase activity |
Functional Information from PROSITE/UniProt
| site_id | PS00299 |
| Number of Residues | 26 |
| Details | UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD |
| Chain | Residue | Details |
| u | LYS27-ASP52 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 67 |
| Details | ZN_FING: RING-Gid-type => ECO:0000255|PROSITE-ProRule:PRU01215 |
| Chain | Residue | Details |
| i | CYS314-THR381 | |
| u | ARG72 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | SITE: Essential for ubiquitin ligase activity => ECO:0000250|UniProtKB:P40492 |
| Chain | Residue | Details |
| i | CYS314 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| i | THR28 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457 |
| Chain | Residue | Details |
| u | THR66 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | MOD_RES: ADP-ribosylglycine => ECO:0000269|PubMed:28525742 |
| Chain | Residue | Details |
| u | GLY76 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603 |
| Chain | Residue | Details |
| u | LYS6 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins) |
| Chain | Residue | Details |
| u | GLY76 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144 |
| Chain | Residue | Details |
| u | LYS11 | |
| u | LYS48 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860 |
| Chain | Residue | Details |
| u | LYS27 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127 |
| Chain | Residue | Details |
| u | LYS29 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577 |
| Chain | Residue | Details |
| u | LYS33 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106 |
| Chain | Residue | Details |
| u | LYS63 |
