8OW0

Cryo-EM structure of CBF1-CCAN bound topologically to a centromeric CENP-A nucleosome

Functional Information from GO Data

Chain	GOid	namespace	contents
a	0000070	biological_process	mitotic sister chromatid segregation
a	0000278	biological_process	mitotic cell cycle
a	0000775	cellular_component	chromosome, centromeric region
a	0000779	cellular_component	condensed chromosome, centromeric region
a	0000786	cellular_component	nucleosome
a	0003677	molecular_function	DNA binding
a	0005515	molecular_function	protein binding
a	0005634	cellular_component	nucleus
a	0005694	cellular_component	chromosome
a	0005729	cellular_component	2-micrometer circle DNA
a	0005777	cellular_component	peroxisome
a	0009303	biological_process	rRNA transcription
a	0019237	molecular_function	centromeric DNA binding
a	0030527	molecular_function	structural constituent of chromatin
a	0030543	biological_process	2-micrometer plasmid partitioning
a	0032991	cellular_component	protein-containing complex
a	0043505	cellular_component	CENP-A containing nucleosome
a	0043565	molecular_function	sequence-specific DNA binding
a	0046982	molecular_function	protein heterodimerization activity
a	0051382	biological_process	kinetochore assembly
a	0061644	biological_process	protein localization to CENP-A containing chromatin
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0000775	cellular_component	chromosome, centromeric region
A	0000776	cellular_component	kinetochore
A	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
A	0001227	molecular_function	DNA-binding transcription repressor activity, RNA polymerase II-specific
A	0001228	molecular_function	DNA-binding transcription activator activity, RNA polymerase II-specific
A	0003677	molecular_function	DNA binding
A	0003700	molecular_function	DNA-binding transcription factor activity
A	0005634	cellular_component	nucleus
A	0005694	cellular_component	chromosome
A	0005739	cellular_component	mitochondrion
A	0006338	biological_process	chromatin remodeling
A	0006357	biological_process	regulation of transcription by RNA polymerase II
A	0007059	biological_process	chromosome segregation
A	0019237	molecular_function	centromeric DNA binding
A	0042762	biological_process	regulation of sulfur metabolic process
A	0045944	biological_process	positive regulation of transcription by RNA polymerase II
A	0046983	molecular_function	protein dimerization activity
A	0061431	biological_process	cellular response to methionine
A	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
A	0089713	cellular_component	Cbf1-Met4-Met28 complex
A	1900059	biological_process	positive regulation of sulfate assimilation
A	1900060	biological_process	negative regulation of ceramide biosynthetic process
A	1900090	biological_process	positive regulation of inositol biosynthetic process
b	0000500	cellular_component	RNA polymerase I upstream activating factor complex
b	0000786	cellular_component	nucleosome
b	0003677	molecular_function	DNA binding
b	0005515	molecular_function	protein binding
b	0005634	cellular_component	nucleus
b	0005694	cellular_component	chromosome
b	0006325	biological_process	chromatin organization
b	0006334	biological_process	nucleosome assembly
b	0006355	biological_process	regulation of DNA-templated transcription
b	0030527	molecular_function	structural constituent of chromatin
b	0031298	cellular_component	replication fork protection complex
b	0032991	cellular_component	protein-containing complex
b	0042790	biological_process	nucleolar large rRNA transcription by RNA polymerase I
b	0042802	molecular_function	identical protein binding
b	0045943	biological_process	positive regulation of transcription by RNA polymerase I
b	0046982	molecular_function	protein heterodimerization activity
B	0000122	biological_process	negative regulation of transcription by RNA polymerase II
B	0000775	cellular_component	chromosome, centromeric region
B	0000776	cellular_component	kinetochore
B	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
B	0001227	molecular_function	DNA-binding transcription repressor activity, RNA polymerase II-specific
B	0001228	molecular_function	DNA-binding transcription activator activity, RNA polymerase II-specific
B	0003677	molecular_function	DNA binding
B	0003700	molecular_function	DNA-binding transcription factor activity
B	0005634	cellular_component	nucleus
B	0005694	cellular_component	chromosome
B	0005739	cellular_component	mitochondrion
B	0006338	biological_process	chromatin remodeling
B	0006357	biological_process	regulation of transcription by RNA polymerase II
B	0007059	biological_process	chromosome segregation
B	0019237	molecular_function	centromeric DNA binding
B	0042762	biological_process	regulation of sulfur metabolic process
B	0045944	biological_process	positive regulation of transcription by RNA polymerase II
B	0046983	molecular_function	protein dimerization activity
B	0061431	biological_process	cellular response to methionine
B	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
B	0089713	cellular_component	Cbf1-Met4-Met28 complex
B	1900059	biological_process	positive regulation of sulfate assimilation
B	1900060	biological_process	negative regulation of ceramide biosynthetic process
B	1900090	biological_process	positive regulation of inositol biosynthetic process
c	0000122	biological_process	negative regulation of transcription by RNA polymerase II
c	0000786	cellular_component	nucleosome
c	0003677	molecular_function	DNA binding
c	0005515	molecular_function	protein binding
c	0005634	cellular_component	nucleus
c	0005694	cellular_component	chromosome
c	0006281	biological_process	DNA repair
c	0006325	biological_process	chromatin organization
c	0030527	molecular_function	structural constituent of chromatin
c	0031298	cellular_component	replication fork protection complex
c	0031492	molecular_function	nucleosomal DNA binding
c	0031507	biological_process	heterochromatin formation
c	0032991	cellular_component	protein-containing complex
c	0046982	molecular_function	protein heterodimerization activity
d	0000122	biological_process	negative regulation of transcription by RNA polymerase II
d	0000786	cellular_component	nucleosome
d	0003677	molecular_function	DNA binding
d	0005515	molecular_function	protein binding
d	0005634	cellular_component	nucleus
d	0005694	cellular_component	chromosome
d	0006301	biological_process	postreplication repair
d	0006325	biological_process	chromatin organization
d	0006355	biological_process	regulation of DNA-templated transcription
d	0030527	molecular_function	structural constituent of chromatin
d	0031298	cellular_component	replication fork protection complex
d	0032991	cellular_component	protein-containing complex
d	0046982	molecular_function	protein heterodimerization activity
e	0000070	biological_process	mitotic sister chromatid segregation
e	0000278	biological_process	mitotic cell cycle
e	0000775	cellular_component	chromosome, centromeric region
e	0000779	cellular_component	condensed chromosome, centromeric region
e	0000786	cellular_component	nucleosome
e	0003677	molecular_function	DNA binding
e	0005515	molecular_function	protein binding
e	0005634	cellular_component	nucleus
e	0005694	cellular_component	chromosome
e	0005729	cellular_component	2-micrometer circle DNA
e	0005777	cellular_component	peroxisome
e	0009303	biological_process	rRNA transcription
e	0019237	molecular_function	centromeric DNA binding
e	0030527	molecular_function	structural constituent of chromatin
e	0030543	biological_process	2-micrometer plasmid partitioning
e	0032991	cellular_component	protein-containing complex
e	0043505	cellular_component	CENP-A containing nucleosome
e	0043565	molecular_function	sequence-specific DNA binding
e	0046982	molecular_function	protein heterodimerization activity
e	0051382	biological_process	kinetochore assembly
e	0061644	biological_process	protein localization to CENP-A containing chromatin
f	0000500	cellular_component	RNA polymerase I upstream activating factor complex
f	0000786	cellular_component	nucleosome
f	0003677	molecular_function	DNA binding
f	0005515	molecular_function	protein binding
f	0005634	cellular_component	nucleus
f	0005694	cellular_component	chromosome
f	0006325	biological_process	chromatin organization
f	0006334	biological_process	nucleosome assembly
f	0006355	biological_process	regulation of DNA-templated transcription
f	0030527	molecular_function	structural constituent of chromatin
f	0031298	cellular_component	replication fork protection complex
f	0032991	cellular_component	protein-containing complex
f	0042790	biological_process	nucleolar large rRNA transcription by RNA polymerase I
f	0042802	molecular_function	identical protein binding
f	0045943	biological_process	positive regulation of transcription by RNA polymerase I
f	0046982	molecular_function	protein heterodimerization activity
g	0000122	biological_process	negative regulation of transcription by RNA polymerase II
g	0000786	cellular_component	nucleosome
g	0003677	molecular_function	DNA binding
g	0005515	molecular_function	protein binding
g	0005634	cellular_component	nucleus
g	0005694	cellular_component	chromosome
g	0006281	biological_process	DNA repair
g	0006325	biological_process	chromatin organization
g	0030527	molecular_function	structural constituent of chromatin
g	0031298	cellular_component	replication fork protection complex
g	0031492	molecular_function	nucleosomal DNA binding
g	0031507	biological_process	heterochromatin formation
g	0032991	cellular_component	protein-containing complex
g	0046982	molecular_function	protein heterodimerization activity
h	0000122	biological_process	negative regulation of transcription by RNA polymerase II
h	0000786	cellular_component	nucleosome
h	0003677	molecular_function	DNA binding
h	0005515	molecular_function	protein binding
h	0005634	cellular_component	nucleus
h	0005694	cellular_component	chromosome
h	0006301	biological_process	postreplication repair
h	0006325	biological_process	chromatin organization
h	0006355	biological_process	regulation of DNA-templated transcription
h	0030527	molecular_function	structural constituent of chromatin
h	0031298	cellular_component	replication fork protection complex
h	0032991	cellular_component	protein-containing complex
h	0046982	molecular_function	protein heterodimerization activity
H	0000775	cellular_component	chromosome, centromeric region
H	0000776	cellular_component	kinetochore
H	0003674	molecular_function	molecular_function
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005694	cellular_component	chromosome
H	0007059	biological_process	chromosome segregation
H	0008608	biological_process	attachment of spindle microtubules to kinetochore
H	0034087	biological_process	establishment of mitotic sister chromatid cohesion
H	0051301	biological_process	cell division
H	0051321	biological_process	meiotic cell cycle
I	0000775	cellular_component	chromosome, centromeric region
I	0000776	cellular_component	kinetochore
I	0003674	molecular_function	molecular_function
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005694	cellular_component	chromosome
I	0006270	biological_process	DNA replication initiation
I	0007059	biological_process	chromosome segregation
I	0007094	biological_process	mitotic spindle assembly checkpoint signaling
I	0008608	biological_process	attachment of spindle microtubules to kinetochore
I	0034087	biological_process	establishment of mitotic sister chromatid cohesion
I	0051301	biological_process	cell division
I	0051321	biological_process	meiotic cell cycle
K	0000775	cellular_component	chromosome, centromeric region
K	0000776	cellular_component	kinetochore
K	0003674	molecular_function	molecular_function
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005694	cellular_component	chromosome
K	0007059	biological_process	chromosome segregation
K	0008608	biological_process	attachment of spindle microtubules to kinetochore
K	0034087	biological_process	establishment of mitotic sister chromatid cohesion
K	0051301	biological_process	cell division
K	0051321	biological_process	meiotic cell cycle
L	0000775	cellular_component	chromosome, centromeric region
L	0000776	cellular_component	kinetochore
L	0000940	cellular_component	outer kinetochore
L	0003674	molecular_function	molecular_function
L	0005634	cellular_component	nucleus
L	0005694	cellular_component	chromosome
L	0008608	biological_process	attachment of spindle microtubules to kinetochore
L	0030437	biological_process	ascospore formation
L	0034087	biological_process	establishment of mitotic sister chromatid cohesion
L	0034089	biological_process	establishment of meiotic sister chromatid cohesion
L	0034090	biological_process	maintenance of meiotic sister chromatid cohesion
L	0045144	biological_process	meiotic sister chromatid segregation
L	0051301	biological_process	cell division
L	0051321	biological_process	meiotic cell cycle
L	0071459	biological_process	protein localization to chromosome, centromeric region
N	0000775	cellular_component	chromosome, centromeric region
N	0000776	cellular_component	kinetochore
N	0000940	cellular_component	outer kinetochore
N	0005198	molecular_function	structural molecule activity
N	0005634	cellular_component	nucleus
N	0005694	cellular_component	chromosome
N	0007059	biological_process	chromosome segregation
N	0007094	biological_process	mitotic spindle assembly checkpoint signaling
N	0008608	biological_process	attachment of spindle microtubules to kinetochore
N	0034080	biological_process	CENP-A containing chromatin assembly
N	0034087	biological_process	establishment of mitotic sister chromatid cohesion
N	0034089	biological_process	establishment of meiotic sister chromatid cohesion
N	0034090	biological_process	maintenance of meiotic sister chromatid cohesion
N	0051301	biological_process	cell division
N	0051321	biological_process	meiotic cell cycle
N	0051382	biological_process	kinetochore assembly
N	0071459	biological_process	protein localization to chromosome, centromeric region
O	0000775	cellular_component	chromosome, centromeric region
O	0000776	cellular_component	kinetochore
O	0000817	cellular_component	COMA complex
O	0003674	molecular_function	molecular_function
O	0005634	cellular_component	nucleus
O	0005694	cellular_component	chromosome
O	0008608	biological_process	attachment of spindle microtubules to kinetochore
O	0034087	biological_process	establishment of mitotic sister chromatid cohesion
O	0034508	biological_process	centromere complex assembly
O	0051301	biological_process	cell division
O	0051321	biological_process	meiotic cell cycle
O	0071459	biological_process	protein localization to chromosome, centromeric region
O	1905262	biological_process	negative regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination
P	0000775	cellular_component	chromosome, centromeric region
P	0000776	cellular_component	kinetochore
P	0000817	cellular_component	COMA complex
P	0003674	molecular_function	molecular_function
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005694	cellular_component	chromosome
P	0007059	biological_process	chromosome segregation
P	0007094	biological_process	mitotic spindle assembly checkpoint signaling
P	0008608	biological_process	attachment of spindle microtubules to kinetochore
P	0034087	biological_process	establishment of mitotic sister chromatid cohesion
P	0051301	biological_process	cell division
P	0051321	biological_process	meiotic cell cycle
P	0071459	biological_process	protein localization to chromosome, centromeric region
Q	0000775	cellular_component	chromosome, centromeric region
Q	0000776	cellular_component	kinetochore
Q	0000817	cellular_component	COMA complex
Q	0003674	molecular_function	molecular_function
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005694	cellular_component	chromosome
Q	0008608	biological_process	attachment of spindle microtubules to kinetochore
Q	0051301	biological_process	cell division
Q	0051321	biological_process	meiotic cell cycle
T	0000775	cellular_component	chromosome, centromeric region
T	0000776	cellular_component	kinetochore
T	0005515	molecular_function	protein binding
T	0005634	cellular_component	nucleus
T	0005694	cellular_component	chromosome
T	0007059	biological_process	chromosome segregation
T	0019237	molecular_function	centromeric DNA binding
T	0044877	molecular_function	protein-containing complex binding
T	0051301	biological_process	cell division
T	1905560	biological_process	negative regulation of kinetochore assembly
U	0000775	cellular_component	chromosome, centromeric region
U	0000776	cellular_component	kinetochore
U	0000817	cellular_component	COMA complex
U	0003674	molecular_function	molecular_function
U	0005634	cellular_component	nucleus
U	0005694	cellular_component	chromosome
U	0005737	cellular_component	cytoplasm
U	0005816	cellular_component	spindle pole body
U	0008608	biological_process	attachment of spindle microtubules to kinetochore
U	0034501	biological_process	protein localization to kinetochore
U	0051301	biological_process	cell division
U	0051321	biological_process	meiotic cell cycle
W	0000775	cellular_component	chromosome, centromeric region
W	0000776	cellular_component	kinetochore
W	0005634	cellular_component	nucleus
W	0005694	cellular_component	chromosome
W	0007059	biological_process	chromosome segregation
W	0019237	molecular_function	centromeric DNA binding
Y	0000775	cellular_component	chromosome, centromeric region
Y	0000776	cellular_component	kinetochore
Y	0003674	molecular_function	molecular_function
Y	0005515	molecular_function	protein binding
Y	0005634	cellular_component	nucleus
Y	0005694	cellular_component	chromosome
Y	0008150	biological_process	biological_process
Y	0008608	biological_process	attachment of spindle microtubules to kinetochore
Y	0051301	biological_process	cell division
Y	0051321	biological_process	meiotic cell cycle
Z	0000775	cellular_component	chromosome, centromeric region
Z	0000776	cellular_component	kinetochore
Z	0003674	molecular_function	molecular_function
Z	0005515	molecular_function	protein binding
Z	0005634	cellular_component	nucleus
Z	0005694	cellular_component	chromosome
Z	0007059	biological_process	chromosome segregation
Z	0008608	biological_process	attachment of spindle microtubules to kinetochore
Z	0031511	cellular_component	Mis6-Sim4 complex
Z	0051301	biological_process	cell division
Z	0051321	biological_process	meiotic cell cycle

Functional Information from PROSITE/UniProt

site_id	PS00014
Number of Residues	4
Details	ER_TARGET Endoplasmic reticulum targeting sequence. SQEL

Chain	Residue	Details
g	SER129-LEU132

---

site_id	PS00046
Number of Residues	7
Details	HISTONE_H2A Histone H2A signature. AGLtFPV

Chain	Residue	Details
g	ALA23-VAL29

---

site_id	PS00047
Number of Residues	5
Details	HISTONE_H4 Histone H4 signature. GAKRH

Chain	Residue	Details
b	GLY15-HIS19

---

site_id	PS00357
Number of Residues	23
Details	HISTONE_H2B Histone H2B signature. REIQTavRlILpGELaKHAVSEG

Chain	Residue	Details
d	ARG96-GLY118

---

site_id	PS00959
Number of Residues	9
Details	HISTONE_H3_2 Histone H3 signature 2. PFaRLVKEV

Chain	Residue	Details
e	PRO157-VAL165

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: N-acetylserine => ECO:0007744|PubMed:22814378

Chain	Residue	Details
I	SER2
B	MET1
h	LYS7
h	LYS8
T	SER177
T	SER192
T	SER268

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphoserine; by ATM or ATR => ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198

Chain	Residue	Details
A	SER45
B	SER45
b	LYS13
f	LYS6
f	LYS9
f	LYS13

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198

Chain	Residue	Details
A	SER48
B	SER48
T	SER74

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956

Chain	Residue	Details
A	SER84
B	SER84
f	LYS32
f	LYS78

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:19779198

Chain	Residue	Details
A	THR138
B	THR138
c	LYS14
c	LYS22
T	THR174

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: N5-methylglutamine => ECO:0000269|PubMed:24352239

Chain	Residue	Details
g	GLN106
c	GLN106

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: N6-malonyllysine; alternate => ECO:0000269|PubMed:22389435

Chain	Residue	Details
g	LYS120
c	LYS120

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:11140636

Chain	Residue	Details
g	SER129
c	SER129
h	LYS35
h	LYS47

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Chain	Residue	Details
g	LYS127
h	LYS38
c	LYS127

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate

Chain	Residue	Details
d	LYS7
d	LYS8
h	LYS7
h	LYS8

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:10642555, ECO:0000269|PubMed:12535538, ECO:0000269|PubMed:12535539, ECO:0000269|PubMed:14660635, ECO:0000269|PubMed:15280549, ECO:0000269|PubMed:16432255

Chain	Residue	Details
d	LYS124
h	LYS124

---

site_id	SWS_FT_FI12
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate => ECO:0000305

Chain	Residue	Details
d	LYS17
d	LYS18
h	LYS17
h	LYS18

---