8J9V

Cryo-EM structure of the African swine fever virus topoisomerase 2 complexed with Cut02aDNA and etoposide (EDI-1)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000819	biological_process	sister chromatid segregation
A	0003677	molecular_function	DNA binding
A	0003916	molecular_function	DNA topoisomerase activity
A	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
A	0005524	molecular_function	ATP binding
A	0006259	biological_process	DNA metabolic process
A	0006265	biological_process	DNA topological change
A	0030430	cellular_component	host cell cytoplasm
A	0046872	molecular_function	metal ion binding
B	0000819	biological_process	sister chromatid segregation
B	0003677	molecular_function	DNA binding
B	0003916	molecular_function	DNA topoisomerase activity
B	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
B	0005524	molecular_function	ATP binding
B	0006259	biological_process	DNA metabolic process
B	0006265	biological_process	DNA topological change
B	0030430	cellular_component	host cell cytoplasm
B	0046872	molecular_function	metal ion binding

Functional Information from PROSITE/UniProt

site_id	PS00177
Number of Residues	9
Details	TOPOISOMERASE_II DNA topoisomerase II signature. AAEGDSALS

Chain	Residue	Details
A	ALA436-SER444

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: O-(5'-phospho-DNA)-tyrosine intermediate => ECO:0000255|PROSITE-ProRule:PRU01384, ECO:0000303|PubMed:25463606

Chain	Residue	Details
A	TYR800
B	TYR800

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site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	ASN64
A	ASN95
B	ASN64
B	ASN95

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site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000303|PubMed:25463606

Chain	Residue	Details
A	GLY142
B	GLY142

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site_id	SWS_FT_FI4
Number of Residues	6
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P11388

Chain	Residue	Details
A	GLU438
A	ASP539
A	ASP541
B	GLU438
B	ASP539
B	ASP541

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site_id	SWS_FT_FI5
Number of Residues	2
Details	SITE: Transition state stabilizer => ECO:0000250

Chain	Residue	Details
A	ARG799
B	ARG799

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