8FHQ
Cryo-EM structure of human NCC (class 3-2)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0006811 | biological_process | monoatomic ion transport |
| A | 0015377 | molecular_function | chloride:monoatomic cation symporter activity |
| A | 0016020 | cellular_component | membrane |
| A | 0022857 | molecular_function | transmembrane transporter activity |
| A | 0055085 | biological_process | transmembrane transport |
| B | 0006811 | biological_process | monoatomic ion transport |
| B | 0015377 | molecular_function | chloride:monoatomic cation symporter activity |
| B | 0016020 | cellular_component | membrane |
| B | 0022857 | molecular_function | transmembrane transporter activity |
| B | 0055085 | biological_process | transmembrane transport |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 10 |
| Details | MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P55013 |
| Chain | Residue | Details |
| A | THR54 | |
| B | THR81 | |
| A | THR58 | |
| A | THR63 | |
| A | THR68 | |
| A | THR81 | |
| B | THR54 | |
| B | THR58 | |
| B | THR63 | |
| B | THR68 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 58 |
| Details | TRANSMEM: Discontinuously helical; Name=1 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | VAL229-ASN258 | |
| B | VAL229-ASN258 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 42 |
| Details | TRANSMEM: Helical; Name=2 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | ASP259-GLU280 | |
| B | ASP259-GLU280 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1024 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | TRP281-ASP311 | |
| B | LEU600-GLN1021 | |
| A | SER361-ILE371 | |
| A | LYS478-ARG507 | |
| A | PHE545-LYS567 | |
| A | LEU600-GLN1021 | |
| B | TRP281-ASP311 | |
| B | SER361-ILE371 | |
| B | LYS478-ARG507 | |
| B | PHE545-LYS567 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 44 |
| Details | TRANSMEM: Helical; Name=3 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | LYS312-ARG334 | |
| B | LYS312-ARG334 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 148 |
| Details | TOPO_DOM: Extracellular => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | GLY335-ILE346 | |
| A | GLY394-PHE453 | |
| A | ALA523-THR527 | |
| B | GLY335-ILE346 | |
| B | GLY394-PHE453 | |
| B | ALA523-THR527 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 48 |
| Details | TRANSMEM: Helical; Name=4 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | PHE347-ILE371 | |
| B | PHE347-ILE371 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 44 |
| Details | TRANSMEM: Helical; Name=5 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | TRP281-GLY303 | |
| B | TRP281-GLY303 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 68 |
| Details | TOPO_DOM: Extracellular => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0000305, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | THR304-GLY338 | |
| B | THR304-GLY338 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 42 |
| Details | TRANSMEM: Discontinuously helical; Name=6 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | THR339-ILE360 | |
| B | THR339-ILE360 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 42 |
| Details | TRANSMEM: Helical; Name=7 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | PRO372-ILE393 | |
| B | PRO372-ILE393 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 46 |
| Details | TRANSMEM: Helical; Name=8 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | ALA454-ALA477 | |
| B | ALA454-ALA477 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 28 |
| Details | TRANSMEM: Helical; Name=9 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | GLY508-ILE522 | |
| B | GLY508-ILE522 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 32 |
| Details | TRANSMEM: Helical; Name=10 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | ILE528-ASN544 | |
| B | ILE528-ASN544 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 38 |
| Details | TRANSMEM: Helical; Name=11 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | TRP568-TRP587 | |
| B | TRP568-TRP587 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 22 |
| Details | TRANSMEM: Helical; Name=12 => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | ALA588-LEU599 | |
| B | ALA588-LEU599 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 10 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:36351028, ECO:0000269|PubMed:36792826, ECO:0007744|PDB:7Y6I, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | ALA239 | |
| B | SER468 | |
| A | VAL242 | |
| A | ALA464 | |
| A | SER467 | |
| A | SER468 | |
| B | ALA239 | |
| B | VAL242 | |
| B | ALA464 | |
| B | SER467 |
| site_id | SWS_FT_FI18 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:36792826, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR |
| Chain | Residue | Details |
| A | ALA240 | |
| B | ALA240 |
| site_id | SWS_FT_FI19 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:36792826, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ |
| Chain | Residue | Details |
| A | PHE318 | |
| B | PHE318 |
| site_id | SWS_FT_FI20 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:36792826, ECO:0007744|PDB:8FHN, ECO:0007744|PDB:8FHO, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHQ, ECO:0007744|PDB:8FHR |
| Chain | Residue | Details |
| A | PHE325 | |
| B | PHE325 |
| site_id | SWS_FT_FI21 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:36792826, ECO:0007744|PDB:8FHN, ECO:0007744|PDB:8FHP, ECO:0007744|PDB:8FHR |
| Chain | Residue | Details |
| A | THR352 | |
| B | THR352 |
| site_id | SWS_FT_FI22 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:36351028, ECO:0000305|PubMed:36792826, ECO:0007744|PDB:8FHT |
| Chain | Residue | Details |
| A | GLY353 | |
| A | ILE354 | |
| A | LEU355 | |
| A | TYR540 | |
| B | GLY353 | |
| B | ILE354 | |
| B | LEU355 | |
| B | TYR540 |
| site_id | SWS_FT_FI23 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:36792826, ECO:0007744|PDB:8FHN |
| Chain | Residue | Details |
| A | ASN359 | |
| B | ASN359 |
| site_id | SWS_FT_FI24 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:36792826, ECO:0007744|PDB:8FHN, ECO:0007744|PDB:8FHO |
| Chain | Residue | Details |
| A | LEU648 | |
| B | GLY741 | |
| B | LEU780 | |
| B | ASN781 | |
| A | ARG655 | |
| A | VAL677 | |
| A | GLY741 | |
| A | LEU780 | |
| A | ASN781 | |
| B | LEU648 | |
| B | ARG655 | |
| B | VAL677 |
| site_id | SWS_FT_FI25 |
| Number of Residues | 8 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P59158 |
| Chain | Residue | Details |
| A | VAL134 | |
| A | LYS140 | |
| A | ALA164 | |
| A | GLY217 | |
| B | VAL134 | |
| B | LYS140 | |
| B | ALA164 | |
| B | GLY217 |
| site_id | SWS_FT_FI26 |
| Number of Residues | 6 |
| Details | MOD_RES: Phosphothreonine; by OXSR1 and STK39 => ECO:0000269|PubMed:18270262 |
| Chain | Residue | Details |
| A | GLY137 | |
| A | CYS146 | |
| A | TRP151 | |
| B | GLY137 | |
| B | CYS146 | |
| B | TRP151 |
| site_id | SWS_FT_FI27 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P59158 |
| Chain | Residue | Details |
| A | GLY141 | |
| A | LEU215 | |
| B | GLY141 | |
| B | LEU215 |
| site_id | SWS_FT_FI28 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine; by OXSR1 and STK39 => ECO:0000305|PubMed:18270262, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | THR182 | |
| B | THR182 |
| site_id | SWS_FT_FI29 |
| Number of Residues | 4 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:36351028, ECO:0007744|PDB:7Y6I |
| Chain | Residue | Details |
| A | ASN406 | |
| A | ASN426 | |
| B | ASN406 | |
| B | ASN426 |
