Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8FHQ

Cryo-EM structure of human NCC (class 3-2)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0006811	biological_process	monoatomic ion transport
A	0015377	molecular_function	chloride:monoatomic cation symporter activity
A	0016020	cellular_component	membrane
A	0022857	molecular_function	transmembrane transporter activity
A	0055085	biological_process	transmembrane transport
B	0006811	biological_process	monoatomic ion transport
B	0015377	molecular_function	chloride:monoatomic cation symporter activity
B	0016020	cellular_component	membrane
B	0022857	molecular_function	transmembrane transporter activity
B	0055085	biological_process	transmembrane transport

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	50
Details	Transmembrane: {"description":"Discontinuously helical; Name=1","evidences":[{"source":"PubMed","id":"31367042","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NPL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	30
Details	Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"31367042","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NPL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	44
Details	Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"PubMed","id":"31367042","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NPL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	146
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"31367042","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NPL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	56
Details	Transmembrane: {"description":"Helical; Name=3","evidences":[{"source":"PubMed","id":"31367042","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NPL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	46
Details	Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"PubMed","id":"31367042","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NPL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	48
Details	Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"PubMed","id":"31367042","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NPL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	38
Details	Transmembrane: {"description":"Discontinuously helical; Name=6","evidences":[{"source":"PubMed","id":"31367042","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NPL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	46
Details	Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"PubMed","id":"31367042","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NPL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	54
Details	Transmembrane: {"description":"Helical; Name=8","evidences":[{"source":"PubMed","id":"31367042","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NPL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	36
Details	Transmembrane: {"description":"Helical; Name=9","evidences":[{"source":"PubMed","id":"31367042","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NPL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	46
Details	Transmembrane: {"description":"Helical; Name=10","evidences":[{"source":"PubMed","id":"31367042","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NPL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	38
Details	Transmembrane: {"description":"Helical; Name=11","evidences":[{"source":"PubMed","id":"31367042","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NPL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	30
Details	Transmembrane: {"description":"Helical; Name=12","evidences":[{"source":"PubMed","id":"31367042","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6NPL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI16
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI17
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PDB","id":"6NPH","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6NPL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI18
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PDB","id":"6NPL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI19
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P55011","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI20
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8FHN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI21
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8FHN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI22
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI23
Number of Residues	4
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI24
Number of Residues	2
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PROSITE-ProRule","id":"PRU00498","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI25
Number of Residues	58
Details	Transmembrane: {"description":"Discontinuously helical; Name=1","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI26
Number of Residues	42
Details	Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI27
Number of Residues	182
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI28
Number of Residues	44
Details	Transmembrane: {"description":"Helical; Name=3","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI29
Number of Residues	148
Details	Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI30
Number of Residues	48
Details	Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI31
Number of Residues	44
Details	Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI32
Number of Residues	68
Details	Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"evidenceCode":"ECO:0000305"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI33
Number of Residues	42
Details	Transmembrane: {"description":"Discontinuously helical; Name=6","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI34
Number of Residues	42
Details	Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI35
Number of Residues	46
Details	Transmembrane: {"description":"Helical; Name=8","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI36
Number of Residues	28
Details	Transmembrane: {"description":"Helical; Name=9","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI37
Number of Residues	32
Details	Transmembrane: {"description":"Helical; Name=10","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI38
Number of Residues	38
Details	Transmembrane: {"description":"Helical; Name=11","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI39
Number of Residues	22
Details	Transmembrane: {"description":"Helical; Name=12","evidences":[{"source":"PubMed","id":"36351028","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7Y6I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHT","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI40
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8FHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8FHQ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI41
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36792826","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8FHN","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)