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8FCA

Cryo-EM structure of the human TRPV4 - RhoA in complex with 4alpha-Phorbol 12,13-didecanoate

Functional Information from GO Data
ChainGOidnamespacecontents
A0000122biological_processnegative regulation of transcription by RNA polymerase II
A0001666biological_processresponse to hypoxia
A0002024biological_processdiet induced thermogenesis
A0003779molecular_functionactin binding
A0005034molecular_functionosmosensor activity
A0005080molecular_functionprotein kinase C binding
A0005216molecular_functionmonoatomic ion channel activity
A0005261molecular_functionmonoatomic cation channel activity
A0005262molecular_functioncalcium channel activity
A0005515molecular_functionprotein binding
A0005516molecular_functioncalmodulin binding
A0005524molecular_functionATP binding
A0005783cellular_componentendoplasmic reticulum
A0005881cellular_componentcytoplasmic microtubule
A0005886cellular_componentplasma membrane
A0005912cellular_componentadherens junction
A0005925cellular_componentfocal adhesion
A0005929cellular_componentcilium
A0006811biological_processmonoatomic ion transport
A0006816biological_processcalcium ion transport
A0006874biological_processintracellular calcium ion homeostasis
A0006884biological_processcell volume homeostasis
A0006970biological_processresponse to osmotic stress
A0006971biological_processhypotonic response
A0007015biological_processactin filament organization
A0007043biological_processcell-cell junction assembly
A0007204biological_processpositive regulation of cytosolic calcium ion concentration
A0007231biological_processosmosensory signaling pathway
A0008017molecular_functionmicrotubule binding
A0008289molecular_functionlipid binding
A0009612biological_processresponse to mechanical stimulus
A0009986cellular_componentcell surface
A0010628biological_processpositive regulation of gene expression
A0010759biological_processpositive regulation of macrophage chemotaxis
A0010977biological_processnegative regulation of neuron projection development
A0015275molecular_functionstretch-activated, monoatomic cation-selective, calcium channel activity
A0016020cellular_componentmembrane
A0016324cellular_componentapical plasma membrane
A0019901molecular_functionprotein kinase binding
A0030027cellular_componentlamellipodium
A0030036biological_processactin cytoskeleton organization
A0030103biological_processvasopressin secretion
A0030175cellular_componentfilopodium
A0030426cellular_componentgrowth cone
A0030864cellular_componentcortical actin cytoskeleton
A0031117biological_processpositive regulation of microtubule depolymerization
A0032587cellular_componentruffle membrane
A0032755biological_processpositive regulation of interleukin-6 production
A0032868biological_processresponse to insulin
A0034605biological_processcellular response to heat
A0042169molecular_functionSH2 domain binding
A0042538biological_processhyperosmotic salinity response
A0042593biological_processglucose homeostasis
A0042802molecular_functionidentical protein binding
A0043014molecular_functionalpha-tubulin binding
A0043117biological_processpositive regulation of vascular permeability
A0043622biological_processcortical microtubule organization
A0045989biological_processpositive regulation of striated muscle contraction
A0046330biological_processpositive regulation of JNK cascade
A0046785biological_processmicrotubule polymerization
A0046872molecular_functionmetal ion binding
A0047484biological_processregulation of response to osmotic stress
A0048487molecular_functionbeta-tubulin binding
A0050729biological_processpositive regulation of inflammatory response
A0050891biological_processmulticellular organismal-level water homeostasis
A0051015molecular_functionactin filament binding
A0055085biological_processtransmembrane transport
A0060351biological_processcartilage development involved in endochondral bone morphogenesis
A0070161cellular_componentanchoring junction
A0070374biological_processpositive regulation of ERK1 and ERK2 cascade
A0070509biological_processcalcium ion import
A0070588biological_processcalcium ion transmembrane transport
A0071470biological_processcellular response to osmotic stress
A0071476biological_processcellular hypotonic response
A0071477biological_processcellular hypotonic salinity response
A0071639biological_processpositive regulation of monocyte chemotactic protein-1 production
A0071642biological_processpositive regulation of macrophage inflammatory protein 1 alpha production
A0071651biological_processpositive regulation of chemokine (C-C motif) ligand 5 production
A0097009biological_processenergy homeostasis
A0097497biological_processblood vessel endothelial cell delamination
A1902656biological_processcalcium ion import into cytosol
A1903444biological_processnegative regulation of brown fat cell differentiation
A1903715biological_processregulation of aerobic respiration
A2000340biological_processpositive regulation of chemokine (C-X-C motif) ligand 1 production
B0000122biological_processnegative regulation of transcription by RNA polymerase II
B0001666biological_processresponse to hypoxia
B0002024biological_processdiet induced thermogenesis
B0003779molecular_functionactin binding
B0005034molecular_functionosmosensor activity
B0005080molecular_functionprotein kinase C binding
B0005216molecular_functionmonoatomic ion channel activity
B0005261molecular_functionmonoatomic cation channel activity
B0005262molecular_functioncalcium channel activity
B0005515molecular_functionprotein binding
B0005516molecular_functioncalmodulin binding
B0005524molecular_functionATP binding
B0005783cellular_componentendoplasmic reticulum
B0005881cellular_componentcytoplasmic microtubule
B0005886cellular_componentplasma membrane
B0005912cellular_componentadherens junction
B0005925cellular_componentfocal adhesion
B0005929cellular_componentcilium
B0006811biological_processmonoatomic ion transport
B0006816biological_processcalcium ion transport
B0006874biological_processintracellular calcium ion homeostasis
B0006884biological_processcell volume homeostasis
B0006970biological_processresponse to osmotic stress
B0006971biological_processhypotonic response
B0007015biological_processactin filament organization
B0007043biological_processcell-cell junction assembly
B0007204biological_processpositive regulation of cytosolic calcium ion concentration
B0007231biological_processosmosensory signaling pathway
B0008017molecular_functionmicrotubule binding
B0008289molecular_functionlipid binding
B0009612biological_processresponse to mechanical stimulus
B0009986cellular_componentcell surface
B0010628biological_processpositive regulation of gene expression
B0010759biological_processpositive regulation of macrophage chemotaxis
B0010977biological_processnegative regulation of neuron projection development
B0015275molecular_functionstretch-activated, monoatomic cation-selective, calcium channel activity
B0016020cellular_componentmembrane
B0016324cellular_componentapical plasma membrane
B0019901molecular_functionprotein kinase binding
B0030027cellular_componentlamellipodium
B0030036biological_processactin cytoskeleton organization
B0030103biological_processvasopressin secretion
B0030175cellular_componentfilopodium
B0030426cellular_componentgrowth cone
B0030864cellular_componentcortical actin cytoskeleton
B0031117biological_processpositive regulation of microtubule depolymerization
B0032587cellular_componentruffle membrane
B0032755biological_processpositive regulation of interleukin-6 production
B0032868biological_processresponse to insulin
B0034605biological_processcellular response to heat
B0042169molecular_functionSH2 domain binding
B0042538biological_processhyperosmotic salinity response
B0042593biological_processglucose homeostasis
B0042802molecular_functionidentical protein binding
B0043014molecular_functionalpha-tubulin binding
B0043117biological_processpositive regulation of vascular permeability
B0043622biological_processcortical microtubule organization
B0045989biological_processpositive regulation of striated muscle contraction
B0046330biological_processpositive regulation of JNK cascade
B0046785biological_processmicrotubule polymerization
B0046872molecular_functionmetal ion binding
B0047484biological_processregulation of response to osmotic stress
B0048487molecular_functionbeta-tubulin binding
B0050729biological_processpositive regulation of inflammatory response
B0050891biological_processmulticellular organismal-level water homeostasis
B0051015molecular_functionactin filament binding
B0055085biological_processtransmembrane transport
B0060351biological_processcartilage development involved in endochondral bone morphogenesis
B0070161cellular_componentanchoring junction
B0070374biological_processpositive regulation of ERK1 and ERK2 cascade
B0070509biological_processcalcium ion import
B0070588biological_processcalcium ion transmembrane transport
B0071470biological_processcellular response to osmotic stress
B0071476biological_processcellular hypotonic response
B0071477biological_processcellular hypotonic salinity response
B0071639biological_processpositive regulation of monocyte chemotactic protein-1 production
B0071642biological_processpositive regulation of macrophage inflammatory protein 1 alpha production
B0071651biological_processpositive regulation of chemokine (C-C motif) ligand 5 production
B0097009biological_processenergy homeostasis
B0097497biological_processblood vessel endothelial cell delamination
B1902656biological_processcalcium ion import into cytosol
B1903444biological_processnegative regulation of brown fat cell differentiation
B1903715biological_processregulation of aerobic respiration
B2000340biological_processpositive regulation of chemokine (C-X-C motif) ligand 1 production
C0000122biological_processnegative regulation of transcription by RNA polymerase II
C0001666biological_processresponse to hypoxia
C0002024biological_processdiet induced thermogenesis
C0003779molecular_functionactin binding
C0005034molecular_functionosmosensor activity
C0005080molecular_functionprotein kinase C binding
C0005216molecular_functionmonoatomic ion channel activity
C0005261molecular_functionmonoatomic cation channel activity
C0005262molecular_functioncalcium channel activity
C0005515molecular_functionprotein binding
C0005516molecular_functioncalmodulin binding
C0005524molecular_functionATP binding
C0005783cellular_componentendoplasmic reticulum
C0005881cellular_componentcytoplasmic microtubule
C0005886cellular_componentplasma membrane
C0005912cellular_componentadherens junction
C0005925cellular_componentfocal adhesion
C0005929cellular_componentcilium
C0006811biological_processmonoatomic ion transport
C0006816biological_processcalcium ion transport
C0006874biological_processintracellular calcium ion homeostasis
C0006884biological_processcell volume homeostasis
C0006970biological_processresponse to osmotic stress
C0006971biological_processhypotonic response
C0007015biological_processactin filament organization
C0007043biological_processcell-cell junction assembly
C0007204biological_processpositive regulation of cytosolic calcium ion concentration
C0007231biological_processosmosensory signaling pathway
C0008017molecular_functionmicrotubule binding
C0008289molecular_functionlipid binding
C0009612biological_processresponse to mechanical stimulus
C0009986cellular_componentcell surface
C0010628biological_processpositive regulation of gene expression
C0010759biological_processpositive regulation of macrophage chemotaxis
C0010977biological_processnegative regulation of neuron projection development
C0015275molecular_functionstretch-activated, monoatomic cation-selective, calcium channel activity
C0016020cellular_componentmembrane
C0016324cellular_componentapical plasma membrane
C0019901molecular_functionprotein kinase binding
C0030027cellular_componentlamellipodium
C0030036biological_processactin cytoskeleton organization
C0030103biological_processvasopressin secretion
C0030175cellular_componentfilopodium
C0030426cellular_componentgrowth cone
C0030864cellular_componentcortical actin cytoskeleton
C0031117biological_processpositive regulation of microtubule depolymerization
C0032587cellular_componentruffle membrane
C0032755biological_processpositive regulation of interleukin-6 production
C0032868biological_processresponse to insulin
C0034605biological_processcellular response to heat
C0042169molecular_functionSH2 domain binding
C0042538biological_processhyperosmotic salinity response
C0042593biological_processglucose homeostasis
C0042802molecular_functionidentical protein binding
C0043014molecular_functionalpha-tubulin binding
C0043117biological_processpositive regulation of vascular permeability
C0043622biological_processcortical microtubule organization
C0045989biological_processpositive regulation of striated muscle contraction
C0046330biological_processpositive regulation of JNK cascade
C0046785biological_processmicrotubule polymerization
C0046872molecular_functionmetal ion binding
C0047484biological_processregulation of response to osmotic stress
C0048487molecular_functionbeta-tubulin binding
C0050729biological_processpositive regulation of inflammatory response
C0050891biological_processmulticellular organismal-level water homeostasis
C0051015molecular_functionactin filament binding
C0055085biological_processtransmembrane transport
C0060351biological_processcartilage development involved in endochondral bone morphogenesis
C0070161cellular_componentanchoring junction
C0070374biological_processpositive regulation of ERK1 and ERK2 cascade
C0070509biological_processcalcium ion import
C0070588biological_processcalcium ion transmembrane transport
C0071470biological_processcellular response to osmotic stress
C0071476biological_processcellular hypotonic response
C0071477biological_processcellular hypotonic salinity response
C0071639biological_processpositive regulation of monocyte chemotactic protein-1 production
C0071642biological_processpositive regulation of macrophage inflammatory protein 1 alpha production
C0071651biological_processpositive regulation of chemokine (C-C motif) ligand 5 production
C0097009biological_processenergy homeostasis
C0097497biological_processblood vessel endothelial cell delamination
C1902656biological_processcalcium ion import into cytosol
C1903444biological_processnegative regulation of brown fat cell differentiation
C1903715biological_processregulation of aerobic respiration
C2000340biological_processpositive regulation of chemokine (C-X-C motif) ligand 1 production
D0000122biological_processnegative regulation of transcription by RNA polymerase II
D0001666biological_processresponse to hypoxia
D0002024biological_processdiet induced thermogenesis
D0003779molecular_functionactin binding
D0005034molecular_functionosmosensor activity
D0005080molecular_functionprotein kinase C binding
D0005216molecular_functionmonoatomic ion channel activity
D0005261molecular_functionmonoatomic cation channel activity
D0005262molecular_functioncalcium channel activity
D0005515molecular_functionprotein binding
D0005516molecular_functioncalmodulin binding
D0005524molecular_functionATP binding
D0005783cellular_componentendoplasmic reticulum
D0005881cellular_componentcytoplasmic microtubule
D0005886cellular_componentplasma membrane
D0005912cellular_componentadherens junction
D0005925cellular_componentfocal adhesion
D0005929cellular_componentcilium
D0006811biological_processmonoatomic ion transport
D0006816biological_processcalcium ion transport
D0006874biological_processintracellular calcium ion homeostasis
D0006884biological_processcell volume homeostasis
D0006970biological_processresponse to osmotic stress
D0006971biological_processhypotonic response
D0007015biological_processactin filament organization
D0007043biological_processcell-cell junction assembly
D0007204biological_processpositive regulation of cytosolic calcium ion concentration
D0007231biological_processosmosensory signaling pathway
D0008017molecular_functionmicrotubule binding
D0008289molecular_functionlipid binding
D0009612biological_processresponse to mechanical stimulus
D0009986cellular_componentcell surface
D0010628biological_processpositive regulation of gene expression
D0010759biological_processpositive regulation of macrophage chemotaxis
D0010977biological_processnegative regulation of neuron projection development
D0015275molecular_functionstretch-activated, monoatomic cation-selective, calcium channel activity
D0016020cellular_componentmembrane
D0016324cellular_componentapical plasma membrane
D0019901molecular_functionprotein kinase binding
D0030027cellular_componentlamellipodium
D0030036biological_processactin cytoskeleton organization
D0030103biological_processvasopressin secretion
D0030175cellular_componentfilopodium
D0030426cellular_componentgrowth cone
D0030864cellular_componentcortical actin cytoskeleton
D0031117biological_processpositive regulation of microtubule depolymerization
D0032587cellular_componentruffle membrane
D0032755biological_processpositive regulation of interleukin-6 production
D0032868biological_processresponse to insulin
D0034605biological_processcellular response to heat
D0042169molecular_functionSH2 domain binding
D0042538biological_processhyperosmotic salinity response
D0042593biological_processglucose homeostasis
D0042802molecular_functionidentical protein binding
D0043014molecular_functionalpha-tubulin binding
D0043117biological_processpositive regulation of vascular permeability
D0043622biological_processcortical microtubule organization
D0045989biological_processpositive regulation of striated muscle contraction
D0046330biological_processpositive regulation of JNK cascade
D0046785biological_processmicrotubule polymerization
D0046872molecular_functionmetal ion binding
D0047484biological_processregulation of response to osmotic stress
D0048487molecular_functionbeta-tubulin binding
D0050729biological_processpositive regulation of inflammatory response
D0050891biological_processmulticellular organismal-level water homeostasis
D0051015molecular_functionactin filament binding
D0055085biological_processtransmembrane transport
D0060351biological_processcartilage development involved in endochondral bone morphogenesis
D0070161cellular_componentanchoring junction
D0070374biological_processpositive regulation of ERK1 and ERK2 cascade
D0070509biological_processcalcium ion import
D0070588biological_processcalcium ion transmembrane transport
D0071470biological_processcellular response to osmotic stress
D0071476biological_processcellular hypotonic response
D0071477biological_processcellular hypotonic salinity response
D0071639biological_processpositive regulation of monocyte chemotactic protein-1 production
D0071642biological_processpositive regulation of macrophage inflammatory protein 1 alpha production
D0071651biological_processpositive regulation of chemokine (C-C motif) ligand 5 production
D0097009biological_processenergy homeostasis
D0097497biological_processblood vessel endothelial cell delamination
D1902656biological_processcalcium ion import into cytosol
D1903444biological_processnegative regulation of brown fat cell differentiation
D1903715biological_processregulation of aerobic respiration
D2000340biological_processpositive regulation of chemokine (C-X-C motif) ligand 1 production
Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues2568
DetailsTOPO_DOM: Cytoplasmic => ECO:0000250|UniProtKB:O35433
ChainResidueDetails
AMET1-VAL469
ALYS535-GLY547
AARG594-LYS608
AGLY723-LEU871
BMET1-VAL469
BLYS535-GLY547
BARG594-LYS608
BGLY723-LEU871
CMET1-VAL469
CLYS535-GLY547
CARG594-LYS608
CGLY723-LEU871
DMET1-VAL469
DLYS535-GLY547
DARG594-LYS608
DGLY723-LEU871

site_idSWS_FT_FI2
Number of Residues564
DetailsTRANSMEM: Helical => ECO:0000250|UniProtKB:O35433
ChainResidueDetails
ASER470-TYR490
ATYR508-MET534
ASER548-LEU568
AALA573-THR593
AILE609-LEU636
AVAL694-VAL722
BSER470-TYR490
BTYR508-MET534
BSER548-LEU568
BALA573-THR593
BILE609-LEU636
BVAL694-VAL722
CSER470-TYR490
CTYR508-MET534
CSER548-LEU568
CALA573-THR593
CILE609-LEU636
CVAL694-VAL722
DSER470-TYR490
DTYR508-MET534
DSER548-LEU568
DALA573-THR593
DILE609-LEU636
DVAL694-VAL722

site_idSWS_FT_FI3
Number of Residues216
DetailsTOPO_DOM: Extracellular => ECO:0000250|UniProtKB:O35433
ChainResidueDetails
ATYR491-ASP507
AALA569-GLU572
AASN637-THR665
ALEU686-VAL693
BTYR491-ASP507
BALA569-GLU572
BASN637-THR665
BLEU686-VAL693
CTYR491-ASP507
CALA569-GLU572
CASN637-THR665
CLEU686-VAL693
DTYR491-ASP507
DALA569-GLU572
DASN637-THR665
DLEU686-VAL693

site_idSWS_FT_FI4
Number of Residues76
DetailsINTRAMEM: Pore-forming => ECO:0000250|UniProtKB:O35433
ChainResidueDetails
APHE666-MET685
BPHE666-MET685
CPHE666-MET685
DPHE666-MET685

site_idSWS_FT_FI5
Number of Residues20
DetailsBINDING: BINDING => ECO:0000269|PubMed:22702953, ECO:0007744|PDB:4DX2
ChainResidueDetails
ALYS192
ALYS197
AASN201
ATYR236
AARG248
BLYS192
BLYS197
BASN201
BTYR236
BARG248
CLYS192
CLYS197
CASN201
CTYR236
CARG248
DLYS192
DLYS197
DASN201
DTYR236
DARG248

site_idSWS_FT_FI6
Number of Residues12
DetailsBINDING: BINDING => ECO:0000250|UniProtKB:A0A1D5PXA5
ChainResidueDetails
AARG249
AASN296
ALYS344
BARG249
BASN296
BLYS344
CARG249
CASN296
CLYS344
DARG249
DASN296
DLYS344

site_idSWS_FT_FI7
Number of Residues4
DetailsBINDING: BINDING => ECO:0000250|UniProtKB:Q9R186
ChainResidueDetails
AASP682
BASP682
CASP682
DASP682

site_idSWS_FT_FI8
Number of Residues12
DetailsMOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:Q9EPK8
ChainResidueDetails
ATYR110
ATYR253
ATYR805
BTYR110
BTYR253
BTYR805
CTYR110
CTYR253
CTYR805
DTYR110
DTYR253
DTYR805

site_idSWS_FT_FI9
Number of Residues4
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q9EPK8
ChainResidueDetails
ASER824
BSER824
CSER824
DSER824

221051

PDB entries from 2024-06-12

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