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8EYY

Cryo-EM structure of 4 insulins bound full-length mouse IR mutant with physically decoupled alpha CTs (C684S/C685S/C687S, denoted as IR-3CS) Asymmetric conformation 2

Functional Information from GO Data
ChainGOidnamespacecontents
A0001540molecular_functionamyloid-beta binding
A0001933biological_processnegative regulation of protein phosphorylation
A0001934biological_processpositive regulation of protein phosphorylation
A0002092biological_processpositive regulation of receptor internalization
A0003007biological_processheart morphogenesis
A0004672molecular_functionprotein kinase activity
A0004713molecular_functionprotein tyrosine kinase activity
A0004714molecular_functiontransmembrane receptor protein tyrosine kinase activity
A0005009molecular_functioninsulin receptor activity
A0005159molecular_functioninsulin-like growth factor receptor binding
A0005515molecular_functionprotein binding
A0005524molecular_functionATP binding
A0005525molecular_functionGTP binding
A0005635cellular_componentnuclear envelope
A0005764cellular_componentlysosome
A0005768cellular_componentendosome
A0005770cellular_componentlate endosome
A0005886cellular_componentplasma membrane
A0005899cellular_componentinsulin receptor complex
A0005901cellular_componentcaveola
A0006355biological_processregulation of DNA-templated transcription
A0006468biological_processprotein phosphorylation
A0006898biological_processreceptor-mediated endocytosis
A0007169biological_processcell surface receptor protein tyrosine kinase signaling pathway
A0007186biological_processG protein-coupled receptor signaling pathway
A0008284biological_processpositive regulation of cell population proliferation
A0008286biological_processinsulin receptor signaling pathway
A0008544biological_processepidermis development
A0008584biological_processmale gonad development
A0009887biological_processanimal organ morphogenesis
A0009897cellular_componentexternal side of plasma membrane
A0010310biological_processregulation of hydrogen peroxide metabolic process
A0010560biological_processpositive regulation of glycoprotein biosynthetic process
A0010629biological_processnegative regulation of gene expression
A0016020cellular_componentmembrane
A0019901molecular_functionprotein kinase binding
A0019903molecular_functionprotein phosphatase binding
A0019904molecular_functionprotein domain specific binding
A0030238biological_processmale sex determination
A0030325biological_processadrenal gland development
A0030335biological_processpositive regulation of cell migration
A0030424cellular_componentaxon
A0030425cellular_componentdendrite
A0031017biological_processexocrine pancreas development
A0031405molecular_functionlipoic acid binding
A0031623biological_processreceptor internalization
A0031667biological_processresponse to nutrient levels
A0031981cellular_componentnuclear lumen
A0031994molecular_functioninsulin-like growth factor I binding
A0031995molecular_functioninsulin-like growth factor II binding
A0032590cellular_componentdendrite membrane
A0032809cellular_componentneuronal cell body membrane
A0032869biological_processcellular response to insulin stimulus
A0034612biological_processresponse to tumor necrosis factor
A0038024molecular_functioncargo receptor activity
A0042327biological_processpositive regulation of phosphorylation
A0042593biological_processglucose homeostasis
A0043025cellular_componentneuronal cell body
A0043235cellular_componentreceptor complex
A0043243biological_processpositive regulation of protein-containing complex disassembly
A0043410biological_processpositive regulation of MAPK cascade
A0043423molecular_function3-phosphoinositide-dependent protein kinase binding
A0043548molecular_functionphosphatidylinositol 3-kinase binding
A0043559molecular_functioninsulin binding
A0043560molecular_functioninsulin receptor substrate binding
A0044297cellular_componentcell body
A0044877molecular_functionprotein-containing complex binding
A0045429biological_processpositive regulation of nitric oxide biosynthetic process
A0045725biological_processpositive regulation of glycogen biosynthetic process
A0045821biological_processpositive regulation of glycolytic process
A0045840biological_processpositive regulation of mitotic nuclear division
A0045893biological_processpositive regulation of DNA-templated transcription
A0045995biological_processregulation of embryonic development
A0046326biological_processpositive regulation of glucose import
A0046718biological_processsymbiont entry into host cell
A0046777biological_processprotein autophosphorylation
A0048639biological_processpositive regulation of developmental growth
A0051425molecular_functionPTB domain binding
A0051446biological_processpositive regulation of meiotic cell cycle
A0051897biological_processpositive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
A0055038cellular_componentrecycling endosome membrane
A0060267biological_processpositive regulation of respiratory burst
A0060417cellular_componentyolk
A0071363biological_processcellular response to growth factor stimulus
A0097062biological_processdendritic spine maintenance
A0097242biological_processamyloid-beta clearance
A1990535biological_processneuron projection maintenance
A2000194biological_processregulation of female gonad development
A2000252biological_processnegative regulation of feeding behavior
B0001540molecular_functionamyloid-beta binding
B0001933biological_processnegative regulation of protein phosphorylation
B0001934biological_processpositive regulation of protein phosphorylation
B0002092biological_processpositive regulation of receptor internalization
B0003007biological_processheart morphogenesis
B0004672molecular_functionprotein kinase activity
B0004713molecular_functionprotein tyrosine kinase activity
B0004714molecular_functiontransmembrane receptor protein tyrosine kinase activity
B0005009molecular_functioninsulin receptor activity
B0005159molecular_functioninsulin-like growth factor receptor binding
B0005515molecular_functionprotein binding
B0005524molecular_functionATP binding
B0005525molecular_functionGTP binding
B0005635cellular_componentnuclear envelope
B0005764cellular_componentlysosome
B0005768cellular_componentendosome
B0005770cellular_componentlate endosome
B0005886cellular_componentplasma membrane
B0005899cellular_componentinsulin receptor complex
B0005901cellular_componentcaveola
B0006355biological_processregulation of DNA-templated transcription
B0006468biological_processprotein phosphorylation
B0006898biological_processreceptor-mediated endocytosis
B0007169biological_processcell surface receptor protein tyrosine kinase signaling pathway
B0007186biological_processG protein-coupled receptor signaling pathway
B0008284biological_processpositive regulation of cell population proliferation
B0008286biological_processinsulin receptor signaling pathway
B0008544biological_processepidermis development
B0008584biological_processmale gonad development
B0009887biological_processanimal organ morphogenesis
B0009897cellular_componentexternal side of plasma membrane
B0010310biological_processregulation of hydrogen peroxide metabolic process
B0010560biological_processpositive regulation of glycoprotein biosynthetic process
B0010629biological_processnegative regulation of gene expression
B0016020cellular_componentmembrane
B0019901molecular_functionprotein kinase binding
B0019903molecular_functionprotein phosphatase binding
B0019904molecular_functionprotein domain specific binding
B0030238biological_processmale sex determination
B0030325biological_processadrenal gland development
B0030335biological_processpositive regulation of cell migration
B0030424cellular_componentaxon
B0030425cellular_componentdendrite
B0031017biological_processexocrine pancreas development
B0031405molecular_functionlipoic acid binding
B0031623biological_processreceptor internalization
B0031667biological_processresponse to nutrient levels
B0031981cellular_componentnuclear lumen
B0031994molecular_functioninsulin-like growth factor I binding
B0031995molecular_functioninsulin-like growth factor II binding
B0032590cellular_componentdendrite membrane
B0032809cellular_componentneuronal cell body membrane
B0032869biological_processcellular response to insulin stimulus
B0034612biological_processresponse to tumor necrosis factor
B0038024molecular_functioncargo receptor activity
B0042327biological_processpositive regulation of phosphorylation
B0042593biological_processglucose homeostasis
B0043025cellular_componentneuronal cell body
B0043235cellular_componentreceptor complex
B0043243biological_processpositive regulation of protein-containing complex disassembly
B0043410biological_processpositive regulation of MAPK cascade
B0043423molecular_function3-phosphoinositide-dependent protein kinase binding
B0043548molecular_functionphosphatidylinositol 3-kinase binding
B0043559molecular_functioninsulin binding
B0043560molecular_functioninsulin receptor substrate binding
B0044297cellular_componentcell body
B0044877molecular_functionprotein-containing complex binding
B0045429biological_processpositive regulation of nitric oxide biosynthetic process
B0045725biological_processpositive regulation of glycogen biosynthetic process
B0045821biological_processpositive regulation of glycolytic process
B0045840biological_processpositive regulation of mitotic nuclear division
B0045893biological_processpositive regulation of DNA-templated transcription
B0045995biological_processregulation of embryonic development
B0046326biological_processpositive regulation of glucose import
B0046718biological_processsymbiont entry into host cell
B0046777biological_processprotein autophosphorylation
B0048639biological_processpositive regulation of developmental growth
B0051425molecular_functionPTB domain binding
B0051446biological_processpositive regulation of meiotic cell cycle
B0051897biological_processpositive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
B0055038cellular_componentrecycling endosome membrane
B0060267biological_processpositive regulation of respiratory burst
B0060417cellular_componentyolk
B0071363biological_processcellular response to growth factor stimulus
B0097062biological_processdendritic spine maintenance
B0097242biological_processamyloid-beta clearance
B1990535biological_processneuron projection maintenance
B2000194biological_processregulation of female gonad development
B2000252biological_processnegative regulation of feeding behavior
C0005179molecular_functionhormone activity
C0005576cellular_componentextracellular region
D0005179molecular_functionhormone activity
D0005576cellular_componentextracellular region
E0005179molecular_functionhormone activity
E0005576cellular_componentextracellular region
F0005179molecular_functionhormone activity
F0005576cellular_componentextracellular region
Functional Information from PROSITE/UniProt
site_idPS00107
Number of Residues29
DetailsPROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGQGSFGMVYeGnakdiikgeaetr.....VAVK
ChainResidueDetails
ALEU992-LYS1020

site_idPS00109
Number of Residues13
DetailsPROTEIN_KINASE_TYR Tyrosine protein kinases specific active-site signature. FVHrDLAARNCMV
ChainResidueDetails
APHE1118-VAL1130

site_idPS00239
Number of Residues9
DetailsRECEPTOR_TYR_KIN_II Receptor tyrosine kinase class II signature. DIYetdYYR
ChainResidueDetails
AASP1146-ARG1154

site_idPS00262
Number of Residues15
DetailsINSULIN Insulin family signature. CCTSiCSlyqLenyC
ChainResidueDetails
CCYS61-CYS75

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues1826
DetailsTOPO_DOM: Extracellular => ECO:0000305
ChainResidueDetails
AHIS1-SER721
ASER726-LYS919
BHIS1-SER721
BSER726-LYS919

site_idSWS_FT_FI2
Number of Residues40
DetailsTRANSMEM: Helical => ECO:0000255
ChainResidueDetails
AILE920-LEU940
BILE920-LEU940

site_idSWS_FT_FI3
Number of Residues808
DetailsTOPO_DOM: Cytoplasmic => ECO:0000305
ChainResidueDetails
APHE941-SER1345
BPHE941-SER1345

site_idSWS_FT_FI4
Number of Residues2
DetailsACT_SITE: Proton donor/acceptor => ECO:0000250
ChainResidueDetails
AASP1122
BASP1122

site_idSWS_FT_FI5
Number of Residues8
DetailsBINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159
ChainResidueDetails
ASER996
AGLU1067
AARG1126
AASP1140
BSER996
BGLU1067
BARG1126
BASP1140

site_idSWS_FT_FI6
Number of Residues2
DetailsBINDING:
ChainResidueDetails
ALYS1020
BLYS1020

site_idSWS_FT_FI7
Number of Residues2
DetailsSITE: Insulin-binding => ECO:0000250
ChainResidueDetails
APHE39
BPHE39

site_idSWS_FT_FI8
Number of Residues4
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P06213
ChainResidueDetails
ASER373
ASER380
BSER373
BSER380

site_idSWS_FT_FI9
Number of Residues2
DetailsMOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P06213
ChainResidueDetails
ATYR374
BTYR374

site_idSWS_FT_FI10
Number of Residues8
DetailsMOD_RES: Phosphotyrosine; by autocatalysis => ECO:0000250|UniProtKB:P06213
ChainResidueDetails
ATYR962
ATYR1148
ATYR1318
ATYR1324
BTYR962
BTYR1148
BTYR1318
BTYR1324

site_idSWS_FT_FI11
Number of Residues4
DetailsMOD_RES: Phosphotyrosine; by autocatalysis => ECO:0000269|PubMed:20655470
ChainResidueDetails
ATYR1152
ATYR1153
BTYR1152
BTYR1153

site_idSWS_FT_FI12
Number of Residues34
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255
ChainResidueDetails
AASN16
AASN514
AASN608
AASN626
AASN673
AASN732
AASN745
AASN883
AASN896
BASN16
BASN25
AASN25
BASN78
BASN111
BASN215
BASN255
BASN295
BASN337
BASN397
BASN514
BASN608
BASN626
AASN78
BASN673
BASN732
BASN745
BASN883
BASN896
AASN111
AASN215
AASN255
AASN295
AASN337
AASN397

site_idSWS_FT_FI13
Number of Residues2
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:19349973, ECO:0000269|PubMed:19656770
ChainResidueDetails
AASN418
BASN418

219515

PDB entries from 2024-05-08

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